中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
Chemical proteomic analysis of the potential toxicological mechanisms of microcystin-RR in zebrafish (Danio rerio) liver

文献类型:期刊论文

作者Tuo, Xun1,2,3; Chen, Jun1; Zhao, Sujuan1,3; Xie, Ping1
刊名ENVIRONMENTAL TOXICOLOGY
出版日期2016-10-01
卷号31期号:10页码:1206-1216
关键词microcystin-RR chemical proteomics toxicological mechanisms zebrafish liver
ISSN号1520-4081
DOI10.1002/tox.22128
通讯作者Xie, Ping(xieping@ihb.ac.cn)
英文摘要Microcystins (MCs) are common toxins produced by freshwater cyanobacteria, and they represent a potential health risk to aquatic organisms and animals, including humans. Specific inhibition of protein phosphatases 1 and 2A is considered the typical mechanism of MCs toxicity, but the exact mechanism has not been fully elucidated. To further our understanding of the toxicological mechanisms induced by MCs, this study is the first to use a chemical proteomic approach to screen proteins that exhibit special interactions with MC-arginine-arginine (MC-RR) from zebrafish (Danio rerio) liver. Seventeen proteins were identified via affinity blocking test. Integration of the results of previous studies and this study revealed that these proteins play a crucial role in various toxic phenomena of liver induced by MCs, such as the disruption of cytoskeleton assembly, oxidative stress, and metabolic disorder. Moreover, in addition to inhibition of protein phosphate activity, the overall toxicity of MCs was simultaneously modulated by the distribution of MCs in cells and their interactions with other target proteins. These results provide new insight into the mechanisms of hepatotoxicity induced by MCs. (c) 2015 Wiley Periodicals, Inc. Environ Toxicol 31: 1206-1216, 2016.
WOS关键词TANDEM MASS-SPECTROMETRY ; AFFINITY-CHROMATOGRAPHY ; RAT HEPATOCYTES ; INTERMEDIATE-FILAMENTS ; PROTEIN PHOSPHATASES ; PROTEOLYTIC-ENZYMES ; CELLULAR-RESPONSES ; SERINE PROTEASES ; MOUSE-LIVER ; FISH LIVER
资助项目National Natural Science Foundation of China[31322013] ; National Natural Science Foundation of China[31070457]
WOS研究方向Environmental Sciences & Ecology ; Toxicology ; Water Resources
语种英语
WOS记录号WOS:000383611900005
出版者WILEY-BLACKWELL
资助机构National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China
源URL[http://ir.ihb.ac.cn/handle/342005/34137]  
专题水生生物研究所_水环境工程研究中心_期刊论文
通讯作者Xie, Ping
作者单位1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Donghu Expt Stn Lake Ecosyst, Donghu South Rd 7, Wuhan 430072, Peoples R China
2.Nanchang Univ, Basic Chem Expt Ctr, Nanchang 330031, Jiangxi, Peoples R China
3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式
GB/T 7714
Tuo, Xun,Chen, Jun,Zhao, Sujuan,et al. Chemical proteomic analysis of the potential toxicological mechanisms of microcystin-RR in zebrafish (Danio rerio) liver[J]. ENVIRONMENTAL TOXICOLOGY,2016,31(10):1206-1216.
APA Tuo, Xun,Chen, Jun,Zhao, Sujuan,&Xie, Ping.(2016).Chemical proteomic analysis of the potential toxicological mechanisms of microcystin-RR in zebrafish (Danio rerio) liver.ENVIRONMENTAL TOXICOLOGY,31(10),1206-1216.
MLA Tuo, Xun,et al."Chemical proteomic analysis of the potential toxicological mechanisms of microcystin-RR in zebrafish (Danio rerio) liver".ENVIRONMENTAL TOXICOLOGY 31.10(2016):1206-1216.

入库方式: OAI收割

来源:水生生物研究所

浏览0
下载0
收藏0
其他版本

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。