A New Paradigm for Producing Astaxanthin From the Unicellular Green Alga Haematococcus pluvialis
文献类型:期刊论文
作者 | Zhang, Zhen1,2; Wang, Baobei2,4; Hu, Qiang3; Sommerfeld, Milton2; Li, Yuanguang1; Han, Danxiang3 |
刊名 | BIOTECHNOLOGY AND BIOENGINEERING |
出版日期 | 2016-10-01 |
卷号 | 113期号:10页码:2088-2099 |
ISSN号 | 0006-3592 |
关键词 | astaxanthin Haematococcus pluvialis heterotrophy acclimation |
DOI | 10.1002/bit.25976 |
英文摘要 | The unicellular green alga Haematococcus pluvialis has been exploited as a cell factory to produce the high-value antioxidant astaxanthin for over two decades, due to its superior ability to synthesize astaxanthin under adverse culture conditions. However, slow vegetative growth under favorable culture conditions and cell deterioration or death under stress conditions (e.g., high light, nitrogen starvation) has limited the astaxanthin production. In this study, a new paradigm that integrated heterotrophic cultivation, acclimation of heterotrophically grown cells to specific light/nutrient regimes, followed by induction of astaxanthin accumulation under photoautotrophic conditions was developed. First, the environmental conditions such as pH, carbon source, nitrogen regime, and light intensity, were optimized to induce astaxanthin accumulation in the dark-grown cells. Althoughmoderate astaxanthin content (e.g., 1% of dry weight) and astaxanthin productivity (2.5mg L-1 day(-1)) were obtained under the optimized conditions, a considerable number of cells died off when subjected to stress for astaxanthin induction. To minimize the susceptibility of dark-grown cells to light stress, the algal cells were acclimated, prior to light induction of astaxanthin biosynthesis, under moderate illumination in the presence of nitrogen. Introduction of this strategy significantly reduced the cell mortality rate under high-light and resulted in increased cellular astaxanthin content and astaxanthin productivity. The productivity of astaxanthin was further improved to 10.5mg L-1 day(-1) by implementation of such a strategy in a bubbling column photobioreactor. Biochemical and physiological analyses suggested that rebuilding of photosynthetic apparatus including D1 protein and PsbO, and recovery of PSII activities, are essential for acclimation of dark-grown cells under photo-induction conditions. (C) 2016 The Authors. Biotechnology and Bioengineering Published by Wiley Periodicals, Inc. |
WOS关键词 | DILUTION-PHOTOINDUCTION CULTIVATION ; DEPENDENT CHLOROPHYLL BIOSYNTHESIS ; CHLAMYDOMONAS-REINHARDTII ; HETEROTROPHIC GROWTH ; FLUORESCENCE ; LACUSTRIS ; CULTURES ; IDENTIFICATION ; CHLOROPLASTS ; MICROALGAE |
资助项目 | State Development & Investment Corporation (SDIC) of China ; National Science Foundation of Hubei Province[2015CFA022] |
WOS研究方向 | Biotechnology & Applied Microbiology |
语种 | 英语 |
出版者 | WILEY-BLACKWELL |
WOS记录号 | WOS:000386753600002 |
资助机构 | State Development & Investment Corporation (SDIC) of China ; State Development & Investment Corporation (SDIC) of China ; State Development & Investment Corporation (SDIC) of China ; State Development & Investment Corporation (SDIC) of China ; State Development & Investment Corporation (SDIC) of China ; State Development & Investment Corporation (SDIC) of China ; State Development & Investment Corporation (SDIC) of China ; State Development & Investment Corporation (SDIC) of China ; National Science Foundation of Hubei Province ; National Science Foundation of Hubei Province ; National Science Foundation of Hubei Province ; National Science Foundation of Hubei Province ; National Science Foundation of Hubei Province ; National Science Foundation of Hubei Province ; National Science Foundation of Hubei Province ; National Science Foundation of Hubei Province ; State Development & Investment Corporation (SDIC) of China ; State Development & Investment Corporation (SDIC) of China ; State Development & Investment Corporation (SDIC) of China ; State Development & Investment Corporation (SDIC) of China ; State Development & Investment Corporation (SDIC) of China ; State Development & Investment Corporation (SDIC) of China ; State Development & Investment Corporation (SDIC) of China ; State Development & Investment Corporation (SDIC) of China ; National Science Foundation of Hubei Province ; National Science Foundation of Hubei Province ; National Science Foundation of Hubei Province ; National Science Foundation of Hubei Province ; National Science Foundation of Hubei Province ; National Science Foundation of Hubei Province ; National Science Foundation of Hubei Province ; National Science Foundation of Hubei Province ; State Development & Investment Corporation (SDIC) of China ; State Development & Investment Corporation (SDIC) of China ; State Development & Investment Corporation (SDIC) of China ; State Development & Investment Corporation (SDIC) of China ; State Development & Investment Corporation (SDIC) of China ; State Development & Investment Corporation (SDIC) of China ; State Development & Investment Corporation (SDIC) of China ; State Development & Investment Corporation (SDIC) of China ; National Science Foundation of Hubei Province ; National Science Foundation of Hubei Province ; National Science Foundation of Hubei Province ; National Science Foundation of Hubei Province ; National Science Foundation of Hubei Province ; National Science Foundation of Hubei Province ; National Science Foundation of Hubei Province ; National Science Foundation of Hubei Province ; State Development & Investment Corporation (SDIC) of China ; State Development & Investment Corporation (SDIC) of China ; State Development & Investment Corporation (SDIC) of China ; State Development & Investment Corporation (SDIC) of China ; State Development & Investment Corporation (SDIC) of China ; State Development & Investment Corporation (SDIC) of China ; State Development & Investment Corporation (SDIC) of China ; State Development & Investment Corporation (SDIC) of China ; National Science Foundation of Hubei Province ; National Science Foundation of Hubei Province ; National Science Foundation of Hubei Province ; National Science Foundation of Hubei Province ; National Science Foundation of Hubei Province ; National Science Foundation of Hubei Province ; National Science Foundation of Hubei Province ; National Science Foundation of Hubei Province ; State Development & Investment Corporation (SDIC) of China ; State Development & Investment Corporation (SDIC) of China ; State Development & Investment Corporation (SDIC) of China ; State Development & Investment Corporation (SDIC) of China ; State Development & Investment Corporation (SDIC) of China ; State Development & Investment Corporation (SDIC) of China ; State Development & Investment Corporation (SDIC) of China ; State Development & Investment Corporation (SDIC) of China ; National Science Foundation of Hubei Province ; National Science Foundation of Hubei Province ; National Science Foundation of Hubei Province ; National Science Foundation of Hubei Province ; National Science Foundation of Hubei Province ; National Science Foundation of Hubei Province ; National Science Foundation of Hubei Province ; National Science Foundation of Hubei Province ; State Development & Investment Corporation (SDIC) of China ; State Development & Investment Corporation (SDIC) of China ; State Development & Investment Corporation (SDIC) of China ; State Development & Investment Corporation (SDIC) of China ; State Development & Investment Corporation (SDIC) of China ; State Development & Investment Corporation (SDIC) of China ; State Development & Investment Corporation (SDIC) of China ; State Development & Investment Corporation (SDIC) of China ; National Science Foundation of Hubei Province ; National Science Foundation of Hubei Province ; National Science Foundation of Hubei Province ; National Science Foundation of Hubei Province ; National Science Foundation of Hubei Province ; National Science Foundation of Hubei Province ; National Science Foundation of Hubei Province ; National Science Foundation of Hubei Province ; State Development & Investment Corporation (SDIC) of China ; State Development & Investment Corporation (SDIC) of China ; State Development & Investment Corporation (SDIC) of China ; State Development & Investment Corporation (SDIC) of China ; State Development & Investment Corporation (SDIC) of China ; State Development & Investment Corporation (SDIC) of China ; State Development & Investment Corporation (SDIC) of China ; State Development & Investment Corporation (SDIC) of China ; National Science Foundation of Hubei Province ; National Science Foundation of Hubei Province ; National Science Foundation of Hubei Province ; National Science Foundation of Hubei Province ; National Science Foundation of Hubei Province ; National Science Foundation of Hubei Province ; National Science Foundation of Hubei Province ; National Science Foundation of Hubei Province ; State Development & Investment Corporation (SDIC) of China ; State Development & Investment Corporation (SDIC) of China ; State Development & Investment Corporation (SDIC) of China ; State Development & Investment Corporation (SDIC) of China ; State Development & Investment Corporation (SDIC) of China ; State Development & Investment Corporation (SDIC) of China ; State Development & Investment Corporation (SDIC) of China ; State Development & Investment Corporation (SDIC) of China ; National Science Foundation of Hubei Province ; National Science Foundation of Hubei Province ; National Science Foundation of Hubei Province ; National Science Foundation of Hubei Province ; National Science Foundation of Hubei Province ; National Science Foundation of Hubei Province ; National Science Foundation of Hubei Province ; National Science Foundation of Hubei Province |
源URL | [http://ir.ihb.ac.cn/handle/342005/34169] |
专题 | 水生生物研究所_藻类生物技术和生物能源研发中心_期刊论文 |
通讯作者 | Li, Yuanguang; Han, Danxiang |
作者单位 | 1.East China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai, Peoples R China 2.Arizona State Univ, Dept Human Syst & Environm, Mesa, AZ USA 3.Chinese Acad Sci, Inst Hydrobiol, Ctr Microalgal Biotechnol & Biofuels, Wuhan, Hubei, Peoples R China 4.Xiamen Univ, Dept Chem & Biochem Engn, Xiamen, Fujian, Peoples R China |
推荐引用方式 GB/T 7714 | Zhang, Zhen,Wang, Baobei,Hu, Qiang,et al. A New Paradigm for Producing Astaxanthin From the Unicellular Green Alga Haematococcus pluvialis[J]. BIOTECHNOLOGY AND BIOENGINEERING,2016,113(10):2088-2099. |
APA | Zhang, Zhen,Wang, Baobei,Hu, Qiang,Sommerfeld, Milton,Li, Yuanguang,&Han, Danxiang.(2016).A New Paradigm for Producing Astaxanthin From the Unicellular Green Alga Haematococcus pluvialis.BIOTECHNOLOGY AND BIOENGINEERING,113(10),2088-2099. |
MLA | Zhang, Zhen,et al."A New Paradigm for Producing Astaxanthin From the Unicellular Green Alga Haematococcus pluvialis".BIOTECHNOLOGY AND BIOENGINEERING 113.10(2016):2088-2099. |
入库方式: OAI收割
来源:水生生物研究所
其他版本
除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。