Detection of Helicobacter in the fecal material of the endangered Yangtze finless porpoise Neophocaena phocaenoides asiaeorientalis
文献类型：期刊论文
| 作者 | McLaughlin, R. W.2; Zheng, J. S.1; Chen, M. M.1,3; Zhao, Q. Z.1; Wang, D.1 |
| 刊名 | DISEASES OF AQUATIC ORGANISMS
 |
| 出版日期 | 2011-07-12 |
| 卷号 | 95期号:3页码:241-245 |
| 关键词 | Helicobacter cetorum Helicobacter spp. Neophocaena phocaenoides asiaeorientalis Yangtze finless porpoise Cetacean |
| ISSN号 | 0177-5103 |
| 通讯作者 | Zheng, JS (reprint author), Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China ; zhengjinsong@ihb.ac.cn |
| 中文摘要 | In animals, infection by the Epsilonproteobacteria Helicobacter spp. and H. cetorum is widespread. It has been suggested that H. cetorum may cause gastritis in cetaceans. The aim of our study was to investigate the presence of Helicobacter spp. in the fecal material of the endangered Yangtze finless porpoise Neophocaena phocaenoides asiaeorientalis. The fecal material of 12 porpoises living in the wild in Poyang Lake and 1 porpoise living in captivity at the Wuhan Baiji Dolphinarium were examined by PCR for the presence of Helicobacter spp. The fecal material of 8 of 12 wild porpoises and the captive porpoise were positive for Helicobacter spp. as determined by PCR using Helicobacter-specific primers, which target the 16S rRNA gene. A 16S rRNA clone library was then prepared from 1 sample isolated from a female porpoise living in the wild. DNA sequence analysis from 3 of the clones showed 98 to 99% identity to the H. cetorum 16S rRNA gene. These results demonstrate the prevalence of Helicobacter spp. and H. cetorum in endangered freshwater finless porpoises. |
| 英文摘要 | In animals, infection by the Epsilonproteobacteria Helicobacter spp. and H. cetorum is widespread. It has been suggested that H. cetorum may cause gastritis in cetaceans. The aim of our study was to investigate the presence of Helicobacter spp. in the fecal material of the endangered Yangtze finless porpoise Neophocaena phocaenoides asiaeorientalis. The fecal material of 12 porpoises living in the wild in Poyang Lake and 1 porpoise living in captivity at the Wuhan Baiji Dolphinarium were examined by PCR for the presence of Helicobacter spp. The fecal material of 8 of 12 wild porpoises and the captive porpoise were positive for Helicobacter spp. as determined by PCR using Helicobacter-specific primers, which target the 16S rRNA gene. A 16S rRNA clone library was then prepared from 1 sample isolated from a female porpoise living in the wild. DNA sequence analysis from 3 of the clones showed 98 to 99% identity to the H. cetorum 16S rRNA gene. These results demonstrate the prevalence of Helicobacter spp. and H. cetorum in endangered freshwater finless porpoises. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 学科主题 | Fisheries; Veterinary Sciences |
| 类目[WOS] | Fisheries ; Veterinary Sciences |
| 研究领域[WOS] | Fisheries ; Veterinary Sciences |
| 关键词[WOS] | INTESTINAL-TRACT ; PYLORI ; CONSERVATION ; DOLPHINS ; SPP. |
| 收录类别 | SCI |
| 资助信息 | National Natural Science Foundation of China[30730018]; National Basic Research Program of China[2007CB411600] |
| 语种 | 英语 |
| WOS记录号 | WOS:000292651700008 |
| 公开日期 | 2011-11-09 |
| 源URL | [http://ir.ihb.ac.cn/handle/342005/16251]  |
| 专题 | 水生生物研究所_水生生物多样性与资源保护研究中心_期刊论文 |
| 作者单位 | 1.Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China 2.St Marys Univ Minnesota, Dept Biochem, Winona, MN 55987 USA 3.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China |
| 推荐引用方式 GB/T 7714 | McLaughlin, R. W.,Zheng, J. S.,Chen, M. M.,et al. Detection of Helicobacter in the fecal material of the endangered Yangtze finless porpoise Neophocaena phocaenoides asiaeorientalis[J]. DISEASES OF AQUATIC ORGANISMS,2011,95(3):241-245. |
| APA | McLaughlin, R. W.,Zheng, J. S.,Chen, M. M.,Zhao, Q. Z.,&Wang, D..(2011).Detection of Helicobacter in the fecal material of the endangered Yangtze finless porpoise Neophocaena phocaenoides asiaeorientalis.DISEASES OF AQUATIC ORGANISMS,95(3),241-245. |
| MLA | McLaughlin, R. W.,et al."Detection of Helicobacter in the fecal material of the endangered Yangtze finless porpoise Neophocaena phocaenoides asiaeorientalis".DISEASES OF AQUATIC ORGANISMS 95.3(2011):241-245. |
入库方式： OAI收割
来源：水生生物研究所
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