Studies on the toxic effects of microcystin-LR on the zebrafish (Danio rerio) under different temperatures
文献类型：期刊论文
| 作者 | Zhang, Xuezhen2,3; Ji, Wei1; Zhang, Huan2,3; Zhang, Wei1,2,3; Xie, Ping1,2,3 |
| 刊名 | JOURNAL OF APPLIED TOXICOLOGY
 |
| 出版日期 | 2011-08-01 |
| 卷号 | 31期号:6页码:561-567 |
| 关键词 | microcystin-LR temperature LD50 glutathione S-transferases real-time PCR zebrafish |
| ISSN号 | 0260-437X |
| 通讯作者 | Xie, P (reprint author), Chinese Acad Sci, Inst Hydrobiol, Donghu Expt Stn Lake Ecosyst, State Key Lab Fresh Water Ecol & Biotechnol China, Donghu S Rd 7, Wuhan 430072, Peoples R China ; xieping@ihb.ac.cn |
| 中文摘要 | It is well known that fish have stronger tolerance than mammals to microcystin (MC) exposure, and such a difference is attributed to their different core body temperatures. However, no in vivo study has been conducted to investigate the effects of temperature on MC-induced toxicity in fish, a typical poikilotherm. Tolerance and detoxification response of zebrafish treated with MC-LR were investigated under three temperatures. The LD(50) values evidently increased with a decline of the temperature (547, 260 and 176 mu g kg(-1) at 12,22 and 32 degrees C, respectively), indicating stronger tolerance of the fish at lower temperatures. Changes in the transcription of glutathione S-transferase (GST) isoforms in the fish were observed, and their sensitivity of response in the transcription of GST mRNA was on the order of 12 > 32 > 22 degrees C. We screened out several GST genes which were more delicate to solve the MC-LR exposure at different temperatures, i.e. GST rho1, al, p1 and theta1 in the 12 degrees C group, and GST zeta1 and p2 in the 22 and 32 degrees C groups. Our findings partly validate the hypothesis that high temperature enhances toxic effects of MCs on poikilotherms. Our studies also indicate that temperature-dependent toxic effects should be taken into account for field toxic assessment of microcystins in fish. Copyright (C) 2010 John Wiley & Sons, Ltd. |
| 英文摘要 | It is well known that fish have stronger tolerance than mammals to microcystin (MC) exposure, and such a difference is attributed to their different core body temperatures. However, no in vivo study has been conducted to investigate the effects of temperature on MC-induced toxicity in fish, a typical poikilotherm. Tolerance and detoxification response of zebrafish treated with MC-LR were investigated under three temperatures. The LD50 values evidently increased with a decline of the temperature (547, 260 and 176 mu g kg(-1) at 12,22 and 32 degrees C, respectively), indicating stronger tolerance of the fish at lower temperatures. Changes in the transcription of glutathione S-transferase (GST) isoforms in the fish were observed, and their sensitivity of response in the transcription of GST mRNA was on the order of 12 > 32 > 22 degrees C. We screened out several GST genes which were more delicate to solve the MC-LR exposure at different temperatures, i.e. GST rho1, al, p1 and theta1 in the 12 degrees C group, and GST zeta1 and p2 in the 22 and 32 degrees C groups. Our findings partly validate the hypothesis that high temperature enhances toxic effects of MCs on poikilotherms. Our studies also indicate that temperature-dependent toxic effects should be taken into account for field toxic assessment of microcystins in fish. Copyright (C) 2010 John Wiley & Sons, Ltd. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 学科主题 | Toxicology |
| 类目[WOS] | Toxicology |
| 研究领域[WOS] | Toxicology |
| 关键词[WOS] | GLUTATHIONE-S-TRANSFERASE ; CARP CYPRINUS-CARPIO ; CYANOBACTERIAL HEPATOTOXIN MICROCYSTIN ; GOLDFISH CARASSIUS-AURATUS ; TROUT ONCORHYNCHUS-MYKISS ; MOLECULAR-CLONING ; OXIDATIVE STRESS ; ALPHA-CLASS ; INTRAPERITONEAL INJECTION ; EXTRACTED MICROCYSTINS |
| 收录类别 | SCI |
| 资助信息 | Huazhong Agricultural University; Ministry of Science and Technology of the People's Republic of China[2007BAC26B02] |
| 语种 | 英语 |
| WOS记录号 | WOS:000294744900008 |
| 公开日期 | 2011-11-09 |
| 源URL | [http://ir.ihb.ac.cn/handle/342005/16277]  |
| 专题 | 水生生物研究所_淡水生态学研究中心_期刊论文 |
| 作者单位 | 1.Chinese Acad Sci, Inst Hydrobiol, Donghu Expt Stn Lake Ecosyst, State Key Lab Fresh Water Ecol & Biotechnol China, Wuhan 430072, Peoples R China 2.Huazhong Agr Univ, Coll Fisheries, Wuhan 430070, Peoples R China 3.Minist Educ, Key Lab Agr Anim Genet Breeding & Reprod, Wuhan 430070, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, Xuezhen,Ji, Wei,Zhang, Huan,et al. Studies on the toxic effects of microcystin-LR on the zebrafish (Danio rerio) under different temperatures[J]. JOURNAL OF APPLIED TOXICOLOGY,2011,31(6):561-567. |
| APA | Zhang, Xuezhen,Ji, Wei,Zhang, Huan,Zhang, Wei,&Xie, Ping.(2011).Studies on the toxic effects of microcystin-LR on the zebrafish (Danio rerio) under different temperatures.JOURNAL OF APPLIED TOXICOLOGY,31(6),561-567. |
| MLA | Zhang, Xuezhen,et al."Studies on the toxic effects of microcystin-LR on the zebrafish (Danio rerio) under different temperatures".JOURNAL OF APPLIED TOXICOLOGY 31.6(2011):561-567. |
入库方式： OAI收割
来源：水生生物研究所
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