Active and silent members in the mlr gene cluster of a microcystin-degrading bacterium isolated from Lake Taihu, China
文献类型：期刊论文
| 作者 | Jiang, Yongguang1,2; Shao, Jihai3; Wu, Xingqiang4; Xu, Yao5; Li, Renhui1 |
| 刊名 | FEMS MICROBIOLOGY LETTERS
 |
| 出版日期 | 2011-09-01 |
| 卷号 | 322期号:2页码:108-114 |
| 关键词 | Novosphingobium degradation efficiency gene expression frame-shift mutation |
| ISSN号 | 0378-1097 |
| 通讯作者 | Li, RH (reprint author), Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Hubei Province, Peoples R China ; reli@ihb.ac.cn |
| 中文摘要 | The microcystin-degrading genes, mlr, are important participants in the degradation process of hepatotoxic microcystins for several bacterial species. However, their expression status during degrading microcystins is still unknown. In order to study this expression process, we isolated a novel microcystin-degrading bacterial strain, sequenced its mlr gene cluster and examined the expression of the mlrA gene at different concentrations of microcystin LR. The expression of mlrA increased slightly at 0.4 mg L(-1), and was significantly upregulated at 2.0 mg L(-1). Frameshift mutations were found in the mlrB* gene, and the mRNA of mlrB* could not be detected in the total RNA extracts of Novosphingobium sp. THN1. We conclude that mlrA is actively involved in the microcystin-degrading process, but mlrB* has lost its activity in this bacterial strain. |
| 英文摘要 | The microcystin-degrading genes, mlr, are important participants in the degradation process of hepatotoxic microcystins for several bacterial species. However, their expression status during degrading microcystins is still unknown. In order to study this expression process, we isolated a novel microcystin-degrading bacterial strain, sequenced its mlr gene cluster and examined the expression of the mlrA gene at different concentrations of microcystin LR. The expression of mlrA increased slightly at 0.4 mg L(-1), and was significantly upregulated at 2.0 mg L(-1). Frameshift mutations were found in the mlrB* gene, and the mRNA of mlrB* could not be detected in the total RNA extracts of Novosphingobium sp. THN1. We conclude that mlrA is actively involved in the microcystin-degrading process, but mlrB* has lost its activity in this bacterial strain. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 学科主题 | Microbiology |
| 类目[WOS] | Microbiology |
| 研究领域[WOS] | Microbiology |
| 关键词[WOS] | SAND FILTER ; WATER ; LR ; DEGRADATION ; CYANOBACTERIA ; BIODEGRADATION |
| 收录类别 | SCI |
| 资助信息 | State Key Basic Research and Development Plan of China[2008CB418002]; National Water Science and Technology Projects[2009ZX07101-013-02]; Chinese Academy of Sciences[082303-1-501] |
| 语种 | 英语 |
| WOS记录号 | WOS:000293793200002 |
| 公开日期 | 2011-11-09 |
| 源URL | [http://ir.ihb.ac.cn/handle/342005/16367]  |
| 专题 | 水生生物研究所_藻类生物学及应用研究中心_期刊论文 |
| 作者单位 | 1.Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Hubei Province, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing, Peoples R China 3.Hunan Agr Univ, Resources & Environm Coll, Changsha, Hunan, Peoples R China 4.Chinese Acad Sci, NE Inst Geog & Agr Ecol, Changchun, Peoples R China 5.Nanjing Normal Univ, Coll Geog Sci, Nanjing, Peoples R China |
| 推荐引用方式 GB/T 7714 | Jiang, Yongguang,Shao, Jihai,Wu, Xingqiang,et al. Active and silent members in the mlr gene cluster of a microcystin-degrading bacterium isolated from Lake Taihu, China[J]. FEMS MICROBIOLOGY LETTERS,2011,322(2):108-114. |
| APA | Jiang, Yongguang,Shao, Jihai,Wu, Xingqiang,Xu, Yao,&Li, Renhui.(2011).Active and silent members in the mlr gene cluster of a microcystin-degrading bacterium isolated from Lake Taihu, China.FEMS MICROBIOLOGY LETTERS,322(2),108-114. |
| MLA | Jiang, Yongguang,et al."Active and silent members in the mlr gene cluster of a microcystin-degrading bacterium isolated from Lake Taihu, China".FEMS MICROBIOLOGY LETTERS 322.2(2011):108-114. |
入库方式： OAI收割
来源：水生生物研究所
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