Identification and characterization of genes encoding two novel LEA proteins in Antarctic and temperate strains of Chlorella vulgaris
文献类型：期刊论文
| 作者 | Liu, Xiaoxiang; Wang, Yali; Gao, Hong; Xu, Xudong |
| 刊名 | GENE
 |
| 出版日期 | 2011-08-15 |
| 卷号 | 482期号:1-2页码:51-58 |
| 关键词 | Chlorella vulgaris Antarctic strain Freeze tolerance Late embryogenesis abundant proteins Cryoprotective activity |
| ISSN号 | 0378-1119 |
| 通讯作者 | Xu, XD (reprint author), Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei, Peoples R China ; xux@ihb.ac.cn |
| 中文摘要 | An Antarctic strain (NJ-7) of Chlorella vulgaris possesses the same 18S rRNA sequence as that of a temperate strain (UTEX259), but shows significantly higher freezing tolerance than the latter. Suppression subtractive hybridization (SSH) was performed to identify genes of intensified expression in NJ-7 relative to UTEX259. Among the genes identified, Ccor1 and Ccor2, co-organized in the same gene cluster Ccor1-Ccor2-Ccor1-Ccor2, showed much higher expression levels in NJ-7 than in UTEX259 at both 20 degrees C and 4 degrees C. As detected by Northern blot and Western blot analyses, the two genes were cold-inducible in NJ-7 but almost not expressed in UTEX259. Their encoded products are predicted to share 55.7% identity to each other and possess physicochemical characteristics similar to that of late embryogenesis abundant (LEA) proteins in plants. The purified recombinant Ccor1 and Ccor2 showed high heat-stability and could act as cryoprotectants to lactate dehydrogenase in vitro. Based on their expression patterns and protein characteristics, we propose that Ccor1 and Ccor2 are two novel LEA proteins and are related to the greatly enhanced freezing tolerance in the Antarctic strain. (C) 2011 Elsevier B.V. All rights reserved. |
| 英文摘要 | An Antarctic strain (NJ-7) of Chlorella vulgaris possesses the same 18S rRNA sequence as that of a temperate strain (UTEX259), but shows significantly higher freezing tolerance than the latter. Suppression subtractive hybridization (SSH) was performed to identify genes of intensified expression in NJ-7 relative to UTEX259. Among the genes identified, Ccor1 and Ccor2, co-organized in the same gene cluster Ccor1-Ccor2-Ccor1-Ccor2, showed much higher expression levels in NJ-7 than in UTEX259 at both 20 degrees C and 4 degrees C. As detected by Northern blot and Western blot analyses, the two genes were cold-inducible in NJ-7 but almost not expressed in UTEX259. Their encoded products are predicted to share 55.7% identity to each other and possess physicochemical characteristics similar to that of late embryogenesis abundant (LEA) proteins in plants. The purified recombinant Ccor1 and Ccor2 showed high heat-stability and could act as cryoprotectants to lactate dehydrogenase in vitro. Based on their expression patterns and protein characteristics, we propose that Ccor1 and Ccor2 are two novel LEA proteins and are related to the greatly enhanced freezing tolerance in the Antarctic strain. (C) 2011 Elsevier B.V. All rights reserved. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 学科主题 | Genetics & Heredity |
| 类目[WOS] | Genetics & Heredity |
| 研究领域[WOS] | Genetics & Heredity |
| 关键词[WOS] | EMBRYOGENESIS ABUNDANT PROTEINS ; ENHANCES FREEZING TOLERANCE ; EFFECTS IN-VITRO ; COLD-ACCLIMATION ; ABSCISIC-ACID ; CRYOPROTECTIVE ACTIVITY ; ARABIDOPSIS-THALIANA ; RESPONSIVE DEHYDRIN ; HIC6 GENE ; DEHYDRATION |
| 收录类别 | SCI |
| 资助信息 | Chinese Academy of Sciences[KSCX2-YW-G-060, KSCX2-SW-332] |
| 语种 | 英语 |
| WOS记录号 | WOS:000293496300007 |
| 公开日期 | 2011-11-09 |
| 源URL | [http://ir.ihb.ac.cn/handle/342005/16369]  |
| 专题 | 水生生物研究所_藻类生物学及应用研究中心_期刊论文 |
| 作者单位 | Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei, Peoples R China |
| 推荐引用方式 GB/T 7714 | Liu, Xiaoxiang,Wang, Yali,Gao, Hong,et al. Identification and characterization of genes encoding two novel LEA proteins in Antarctic and temperate strains of Chlorella vulgaris[J]. GENE,2011,482(1-2):51-58. |
| APA | Liu, Xiaoxiang,Wang, Yali,Gao, Hong,&Xu, Xudong.(2011).Identification and characterization of genes encoding two novel LEA proteins in Antarctic and temperate strains of Chlorella vulgaris.GENE,482(1-2),51-58. |
| MLA | Liu, Xiaoxiang,et al."Identification and characterization of genes encoding two novel LEA proteins in Antarctic and temperate strains of Chlorella vulgaris".GENE 482.1-2(2011):51-58. |
入库方式： OAI收割
来源：水生生物研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。