Cloning and expression analysis of Rsk in Brassica napus induced by Sclerotinia sclerotiorum
文献类型：期刊论文
| 作者 | Zhang, Yu2,3; Hu, Chun-xiang2; Zhang, Cheng-gui4; Gan, Li1 |
| 刊名 | ACTA PHYSIOLOGIAE PLANTARUM
 |
| 出版日期 | 2011-07-01 |
| 卷号 | 33期号:4页码:1277-1283 |
| 关键词 | Brassica napus cDNA-AFLP LRR-RI Northern blotting RT-RACE Sclerotinia sclerotiorum Semiquantitative RT-PCR |
| ISSN号 | 0137-5881 |
| 通讯作者 | Gan, L (reprint author), Huazhong Agr Univ, Coll Plant Sci & Technol, Wuhan 430070, Peoples R China ; ganli@mail.hzau.edu.cn |
| 中文摘要 | Based on the significant differences in the resistance to Sclerotinia sclerotiorum between two Brassica napus cultivars, ZhongR888 and Zhongyou821, near isogenic lines (NILs) were established. The resistance differences between Zhongyou821 and BC(5) progeny (the fifth generation from a cross between Zhongyou821 and ZhongR888 with Zhongyou821 as successive backcross parent) was displayed by cDNA-AFLP and differential transcripts-derived fragments (TDFs). The full-length cDNA of 1,707 bp with 1,323 bp open reading frame (ORF, open box), named Rsk was identified by RT-RACE from the resistance related fragment 40-2 in Zhongyou821. It encoded a protein of 440 amino acid residues, which contained a putative extracellular domain, a transmembrane domain, and an intracellular domain. Analysis of the deduced amino acid sequence of Rsk revealed that it had high homology to Arabidopsis thaliana kinase involved in protein binding, and had a conserved region of LRR-RI, indicating it might be a member of leucine-rich repeats, ribonuclease inhibitor-like subfamily. DNA sequences of 1,685 bp and 1,703 bp without an intron were also identified from Zhongyou821 and BC(5,) respectively. Expression analysis showed that Rsk might play a role in disease resistance pathways. |
| 英文摘要 | Based on the significant differences in the resistance to Sclerotinia sclerotiorum between two Brassica napus cultivars, ZhongR888 and Zhongyou821, near isogenic lines (NILs) were established. The resistance differences between Zhongyou821 and BC(5) progeny (the fifth generation from a cross between Zhongyou821 and ZhongR888 with Zhongyou821 as successive backcross parent) was displayed by cDNA-AFLP and differential transcripts-derived fragments (TDFs). The full-length cDNA of 1,707 bp with 1,323 bp open reading frame (ORF, open box), named Rsk was identified by RT-RACE from the resistance related fragment 40-2 in Zhongyou821. It encoded a protein of 440 amino acid residues, which contained a putative extracellular domain, a transmembrane domain, and an intracellular domain. Analysis of the deduced amino acid sequence of Rsk revealed that it had high homology to Arabidopsis thaliana kinase involved in protein binding, and had a conserved region of LRR-RI, indicating it might be a member of leucine-rich repeats, ribonuclease inhibitor-like subfamily. DNA sequences of 1,685 bp and 1,703 bp without an intron were also identified from Zhongyou821 and BC(5,) respectively. Expression analysis showed that Rsk might play a role in disease resistance pathways. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 学科主题 | Plant Sciences |
| 类目[WOS] | Plant Sciences |
| 研究领域[WOS] | Plant Sciences |
| 关键词[WOS] | NEAR-ISOGENIC LINES ; DIFFERENTIAL GENE-EXPRESSION ; DISEASE RESISTANCE GENE ; CDNA-AFLP ; PROTEIN-KINASES ; RICE ; PATHOGEN ; PLANTS ; IDENTIFICATION ; INHERITANCE |
| 收录类别 | SCI |
| 资助信息 | National Nature Science Foundation of China[30170600]; Hubei Nature Science Foundation[2001ABD113] |
| 语种 | 英语 |
| WOS记录号 | WOS:000291600200023 |
| 公开日期 | 2011-11-09 |
| 源URL | [http://ir.ihb.ac.cn/handle/342005/16379]  |
| 专题 | 水生生物研究所_藻类生物学及应用研究中心_期刊论文 |
| 作者单位 | 1.Huazhong Agr Univ, Coll Plant Sci & Technol, Wuhan 430070, Peoples R China 2.Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China 3.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China 4.Dali Univ, Coll Pharm, Dali 671003, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, Yu,Hu, Chun-xiang,Zhang, Cheng-gui,et al. Cloning and expression analysis of Rsk in Brassica napus induced by Sclerotinia sclerotiorum[J]. ACTA PHYSIOLOGIAE PLANTARUM,2011,33(4):1277-1283. |
| APA | Zhang, Yu,Hu, Chun-xiang,Zhang, Cheng-gui,&Gan, Li.(2011).Cloning and expression analysis of Rsk in Brassica napus induced by Sclerotinia sclerotiorum.ACTA PHYSIOLOGIAE PLANTARUM,33(4),1277-1283. |
| MLA | Zhang, Yu,et al."Cloning and expression analysis of Rsk in Brassica napus induced by Sclerotinia sclerotiorum".ACTA PHYSIOLOGIAE PLANTARUM 33.4(2011):1277-1283. |
入库方式： OAI收割
来源：水生生物研究所
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