Molecular characterization and expression pattern of three zona pellucida 3 genes in the Chinese sturgeon, Acipenser sinensis
文献类型：期刊论文
| 作者 | Chuang-Ju, Li1,3; Qi-Wei, Wei1,3; Xi-Hua, Chen1,3; Li, Zhou2; Hong, Cao4; Fang, Gan1; Jian-Fang, Gui2 |
| 刊名 | FISH PHYSIOLOGY AND BIOCHEMISTRY
 |
| 出版日期 | 2011-09-01 |
| 卷号 | 37期号:3页码:471-484 |
| 关键词 | Chinese sturgeon Zona pellucida protein 3 Gene expression RT-PCR |
| ISSN号 | 0920-1742 |
| 通讯作者 | Xi-Hua, C (reprint author), Chinese Acad Fisheries Sci, Yangtze River Fisheries Res Inst, Key Lab Freshwater Biodivers Conservat & Utilizat, Minist Agr China, Jinzhou 434000, Peoples R China ; chenxh@yfi.ac.cn |
| 中文摘要 | Chinese sturgeon (Acipenser sinensis) is a rare and endangered species and also an important resource for the sturgeon aquaculture industry. SMART cDNAs were synthesized from the ovary of A. sinensis, and the full-length cDNAs of three zona pellucida glycoprotein 3 genes (the new gene named AsZP3) were cloned and sequenced. AsZP3.1, AsZP3.2, and AsZP3.3 were 1,388 base pairs (bp), 1,288, and 1,290 bp in length, respectively, and they could be translated into proteins with 440, 394, and 398 amino acids, respectively. High level of amino acids sequence identity was seen between AsZP3.2 and AsZP3.3 (about 82%), but they share low identity with AsZP3.1 (26 and 23%, respectively). The AsZP3.1 has 42-50% amino acids sequence identity values with other fish and lower values with higher vertebrates (38%); AsZP3.2 and AsZP3.3 shared about 30-44% sequence identity with higher vertebrates and other fish. RT-PCR analysis indicated that AsZP3.1 displayed a wide tissue distribution at the mRNA levels including liver, kidney, spleen, heart, and ovary, but AsZP3.2 and AsZP3.3 mRNAs were expressed exclusively in the gonad. All three AsZP3 mRNAs were not detected during embryogenesis and early larval development; furthermore, they were not detected in the gonads of 1- and 2-year-old Chinese sturgeons. All three AsZP3 mRNAs were detected in the testes of 3-year-old males and in the ovaries of 4- and 5-year-old female Chinese sturgeons. |
| 英文摘要 | Chinese sturgeon (Acipenser sinensis) is a rare and endangered species and also an important resource for the sturgeon aquaculture industry. SMART cDNAs were synthesized from the ovary of A. sinensis, and the full-length cDNAs of three zona pellucida glycoprotein 3 genes (the new gene named AsZP3) were cloned and sequenced. AsZP3.1, AsZP3.2, and AsZP3.3 were 1,388 base pairs (bp), 1,288, and 1,290 bp in length, respectively, and they could be translated into proteins with 440, 394, and 398 amino acids, respectively. High level of amino acids sequence identity was seen between AsZP3.2 and AsZP3.3 (about 82%), but they share low identity with AsZP3.1 (26 and 23%, respectively). The AsZP3.1 has 42-50% amino acids sequence identity values with other fish and lower values with higher vertebrates (38%); AsZP3.2 and AsZP3.3 shared about 30-44% sequence identity with higher vertebrates and other fish. RT-PCR analysis indicated that AsZP3.1 displayed a wide tissue distribution at the mRNA levels including liver, kidney, spleen, heart, and ovary, but AsZP3.2 and AsZP3.3 mRNAs were expressed exclusively in the gonad. All three AsZP3 mRNAs were not detected during embryogenesis and early larval development; furthermore, they were not detected in the gonads of 1- and 2-year-old Chinese sturgeons. All three AsZP3 mRNAs were detected in the testes of 3-year-old males and in the ovaries of 4- and 5-year-old female Chinese sturgeons. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 学科主题 | Biochemistry & Molecular Biology; Fisheries; Physiology |
| 类目[WOS] | Biochemistry & Molecular Biology ; Fisheries ; Physiology |
| 研究领域[WOS] | Biochemistry & Molecular Biology ; Fisheries ; Physiology |
| 关键词[WOS] | EGG ENVELOPE PROTEINS ; STRUCTURAL-ANALYSIS ; ORYZIAS-LATIPES ; SPATIAL-DISTRIBUTION ; ESTROGEN INDUCTION ; SPARUS-AURATA ; INNER LAYER ; FISH ; CLONING ; ZP2 |
| 收录类别 | SCI |
| 资助信息 | National Nonprofit Institute Research Grant of Freshwater Fisheries Research Center; Chinese Academy of Fisheries (CAFS); Yangtze River Fisheries Research Institute, CAFS; foundation of Key Laboratory of Freshwater Biodiversity Conservation; Utilization, Ministry of Agriculture of China[LFBCU0704]; National Natural Science Foundation of China[30571411]; State Key Laboratory of Freshwater Ecology and Biotechnology[2008FB007] |
| 语种 | 英语 |
| WOS记录号 | WOS:000293295800013 |
| 公开日期 | 2011-11-09 |
| 源URL | [http://ir.ihb.ac.cn/handle/342005/16319]  |
| 专题 | 水生生物研究所_鱼类生物学及渔业生物技术研究中心_期刊论文 |
| 作者单位 | 1.Chinese Acad Fisheries Sci, Yangtze River Fisheries Res Inst, Key Lab Freshwater Biodivers Conservat & Utilizat, Minist Agr China, Jinzhou 434000, Peoples R China 2.Chinese Acad Sci, Wuhan Ctr Dev Biol, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China 3.Chinese Acad Fisheries Sci, Freshwater Fisheries Res Ctr, Wuxi 214081, Peoples R China 4.Wuhan Univ, Coll Life Sci, Wuhan 430072, Peoples R China |
| 推荐引用方式 GB/T 7714 | Chuang-Ju, Li,Qi-Wei, Wei,Xi-Hua, Chen,et al. Molecular characterization and expression pattern of three zona pellucida 3 genes in the Chinese sturgeon, Acipenser sinensis[J]. FISH PHYSIOLOGY AND BIOCHEMISTRY,2011,37(3):471-484. |
| APA | Chuang-Ju, Li.,Qi-Wei, Wei.,Xi-Hua, Chen.,Li, Zhou.,Hong, Cao.,...&Jian-Fang, Gui.(2011).Molecular characterization and expression pattern of three zona pellucida 3 genes in the Chinese sturgeon, Acipenser sinensis.FISH PHYSIOLOGY AND BIOCHEMISTRY,37(3),471-484. |
| MLA | Chuang-Ju, Li,et al."Molecular characterization and expression pattern of three zona pellucida 3 genes in the Chinese sturgeon, Acipenser sinensis".FISH PHYSIOLOGY AND BIOCHEMISTRY 37.3(2011):471-484. |
入库方式： OAI收割
来源：水生生物研究所
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