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Fluorescent probe for copper(II) ion based on a rhodamine spirolactame derivative, and its application to fluorescent imaging in living cells

文献类型：期刊论文

作者Yu, Chunwei1,2,3,7; Zhang, Jun4,5; Li, Jinhua1,2,3; Liu, Ping1,2,3; Wei, Peihai6; Chen, Lingxin1,2,3
刊名MICROCHIMICA ACTA
出版日期2011-09-01
卷号174期号:3-4页码:247-255
关键词Copper(II) ions Fluorescent probe Fluorescence enhancement Rhodamine derivatives Fluorescence imaging Living cells
ISSN号0026-3672
产权排序[Yu, CW; Li, JH; Liu, P; Chen, LX] CAS, Key Lab Coastal Zone Environm Proc, Yantai 264003, Peoples R China; [Yu, CW; Li, JH; Liu, P; Chen, LX] Shandong Prov Key Lab Coastal Zone Environm Proc, Yantai 264003, Peoples R China; [Yu, CW; Li, JH; Liu, P; Chen, LX] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China; [Zhang, J] Hainan Med Univ, Sch Trop, Haikou 571101, Peoples R China; [Zhang, J] Hainan Med Univ, Lab Med, Haikou 571101, Peoples R China; [Wei, PH] Qilu Normal Univ, Dept Chem, Jinan 250013, Peoples R China; [Yu, CW] Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China
通讯作者Chen, LX, CAS, Key Lab Coastal Zone Environm Proc, Yantai 264003, Peoples R China. lxchen@yic.ac.cn
中文摘要A fluorescent probe for Cu(II) ion is presented. It is based on the rhodamine fluorophore and exhibits high selectivity and sensitivity for Cu(II) ion in aqueous methanol (2:8, v/v) at pH 7.0. The response is based on a ring opening reaction and formation of a strongly fluorescent 1:1 complex. The response is reversible and linear in the range between 50 nM and 900 nM, with a detection limit of 7.0 nM. The probe was successfully applied to fluorescent imaging of Cu(II) ions in HeLa cells.
英文摘要A fluorescent probe for Cu(II) ion is presented. It is based on the rhodamine fluorophore and exhibits high selectivity and sensitivity for Cu(II) ion in aqueous methanol (2:8, v/v) at pH 7.0. The response is based on a ring opening reaction and formation of a strongly fluorescent 1:1 complex. The response is reversible and linear in the range between 50 nM and 900 nM, with a detection limit of 7.0 nM. The probe was successfully applied to fluorescent imaging of Cu(II) ions in HeLa cells.
学科主题Chemistry
研究领域[WOS]Chemistry
关键词[WOS]AQUEOUS-SOLUTION ; ON FLUORESCENT ; CU2+ IONS ; OFF-ON ; SENSITIVITY ; CHEMOSENSOR ; SELECTIVITY ; CHEMODOSIMETER ; COMPLEXES ; HYDRAZIDE
收录类别SCI
资助信息National Natural Science Foundation of China[20975089]; Department of Science and Technology of Shandong Province of China[2008GG20005005]; Chinese Academy of Sciences[KZCX2-EW-206]; Shandong Province Doctor Fund[2008BS04031]
语种英语
WOS记录号WOS:000293964400007
公开日期2011-11-11
源URL[http://ir.yic.ac.cn/handle/133337/5104]  
专题烟台海岸带研究所_山东省海岸带环境工程技术研究中心
烟台海岸带研究所_中科院海岸带环境过程与生态修复重点实验室
作者单位1.CAS, Key Lab Coastal Zone Environm Proc, Yantai 264003, Peoples R China
2.Shandong Prov Key Lab Coastal Zone Environm Proc, Yantai 264003, Peoples R China
3.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China
4.Hainan Med Univ, Sch Trop, Haikou 571101, Peoples R China
5.Hainan Med Univ, Lab Med, Haikou 571101, Peoples R China
6.Qilu Normal Univ, Dept Chem, Jinan 250013, Peoples R China
7.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China
推荐引用方式
GB/T 7714		 Yu, Chunwei,Zhang, Jun,Li, Jinhua,et al. Fluorescent probe for copper(II) ion based on a rhodamine spirolactame derivative, and its application to fluorescent imaging in living cells[J]. MICROCHIMICA ACTA,2011,174(3-4):247-255.
APA Yu, Chunwei,Zhang, Jun,Li, Jinhua,Liu, Ping,Wei, Peihai,&Chen, Lingxin.(2011).Fluorescent probe for copper(II) ion based on a rhodamine spirolactame derivative, and its application to fluorescent imaging in living cells.MICROCHIMICA ACTA,174(3-4),247-255.
MLA Yu, Chunwei,et al."Fluorescent probe for copper(II) ion based on a rhodamine spirolactame derivative, and its application to fluorescent imaging in living cells".MICROCHIMICA ACTA 174.3-4(2011):247-255.

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来源：烟台海岸带研究所

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