Mechanical Point Loading Induces Cortex Stiffening and Actin Reorganization
文献类型：期刊论文
| 作者 | Long M(龙勉)2,3,4 ; Lv SQ(吕守芹)2,3,4; Hu WH(胡文慧)1,3,4; Chen SB(陈深宝)2,3,4; Hu JR(胡锦荣)2,3,4
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| 刊名 | BIOPHYSICAL JOURNAL
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| 出版日期 | 2019-10-15 |
| 卷号 | 117期号:8页码:1405-1418 |
| ISSN号 | 0006-3495 |
| DOI | 10.1016/j.bpj.2019.09.012 |
| 通讯作者 | Lu, Shouqin(lsq@imech.ac.cn) ; Long, Mian(mlong@imech.ac.cn) |
| 英文摘要 | Global cytoskeleton reorganization is well-recognized when cells are exposed to distinct mechanical stimuli, but the localized responses at a specified region of a cell are still unclear. In this work, we mapped the cell-surface mechanical property of single cells in situ before and after static point loading these cells using atomic force microscopy in PeakForce-Quantitative Nano Mechanics mode. Cell-surface stiffness was elevated at a maximum of 1.35-fold at the vicinity of loading site, indicating an enhanced structural protection of the cortex to the cell. Mechanical modeling also elucidated the structural protection from the stiffened cell cortex, in which 9-15% and 10-19% decrease of maximum stress and strain of the nucleus were obtained. Furthermore, the flat-ended atomic force microscopy probes were used to capture cytoskeleton reorganization after point loading quantitatively, revealing that the larger the applied force and the longer the loading time are, the more pronounced cytoskeleton reorganization is. Also, point loading using a microneedle combined with real-time confocal microscopy uncovered the fast dynamics of actin cytoskeleton reorganization for actin-stained live cells after point loading (<10 s). These results furthered the understandings in the transmission of localized mechanical forces into an adherent cell. |
| 分类号 | 一类/力学重要期刊 |
| WOS关键词 | ATOMIC-FORCE MICROSCOPY ; INDUCED CYTOSKELETAL REORGANIZATION ; CELL STIFFNESS ; CYTOCHALASIN-D ; ENDOTHELIAL-CELLS ; PLASMA-MEMBRANE ; CANCER-CELLS ; STRESS ; DECONDENSATION ; ORGANIZATION |
| 资助项目 | National Key Research and Development Program of China[2016YFA0501601] ; National Natural Science Foundation of China[31627804] ; National Natural Science Foundation of China[91642203] ; National Natural Science Foundation of China[31661143044] ; National Natural Science Foundation of China[31570942] ; Frontier Science Key Project of Chinese Science Academy[QYZDJ-SSW-JSC018] ; Strategic Priority Research Program of Chinese Academy of Sciences[XDB22040101] |
| WOS研究方向 | Biophysics |
| 语种 | 英语 |
| WOS记录号 | WOS:000490144100004 |
| 资助机构 | National Key Research and Development Program of China ; National Natural Science Foundation of China ; Frontier Science Key Project of Chinese Science Academy ; Strategic Priority Research Program of Chinese Academy of Sciences |
| 其他责任者 | Lu, Shouqin ; Long, Mian |
| 源URL | [http://dspace.imech.ac.cn/handle/311007/80670]  |
| 专题 | 力学研究所_国家微重力实验室 |
| 作者单位 | 1.Guizhou Med Univ, Key Lab Biol & Med Engn, Immune Cells & Antibody Engn Res Ctr Guizhou Prov, Guiyang, Guizhou, Peoples R China 2.Univ Chinese Acad Sci, Sch Engn Sci, Beijing, Peoples R China; 3.Chinese Acad Sci, CAS Ctr Excellence Complex Syst Mech, Inst Mech, Beijing, Peoples R China; 4.Chinese Acad Sci, Inst Mech, Ctr Biomech & Bioengn, Key Lab Micrograv,Natl Micrograv Lab,Beijing Key, Beijing, Peoples R China; |
| 推荐引用方式 GB/T 7714 | Long M,Lv SQ,Hu WH,et al. Mechanical Point Loading Induces Cortex Stiffening and Actin Reorganization[J]. BIOPHYSICAL JOURNAL,2019,117(8):1405-1418. |
| APA | 龙勉,吕守芹,胡文慧,陈深宝,&胡锦荣.(2019).Mechanical Point Loading Induces Cortex Stiffening and Actin Reorganization.BIOPHYSICAL JOURNAL,117(8),1405-1418. |
| MLA | 龙勉,et al."Mechanical Point Loading Induces Cortex Stiffening and Actin Reorganization".BIOPHYSICAL JOURNAL 117.8(2019):1405-1418. |
入库方式： OAI收割
来源：力学研究所
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