Ratiometric Surface Enhanced Raman Scattering Immunosorbent Assay of Allergenic Proteins via Covalent Organic Framework Composite Material Based Nanozyme Tag Triggered Raman Signal "Turn-on" and Amplification
文献类型：期刊论文
| 作者 | Su, Yiyun; Wu, Di; Chen, Jian; Chen, Guang; Hu, Na ; Wang, Honglun; Wang, Panxue; Han, Haoyu; Li, Guoliang; Wut, Yongning
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| 刊名 | ANALYTICAL CHEMISTRY
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| 出版日期 | 2019 |
| 卷号 | 91期号:18 |
| 英文摘要 | The exploration of nanomaterials with mimic enzyme activity (named nanozyme) has gained extensive attention in the fields of advanced analytical chemistry and materials science. Herein, the gold nanoparticles doped covalent organic frameworks (COFs) were prepared, which exhibited not only excellent mimic nitroreductase activity but also robust stability. By replacing the traditional natural enzyme tag in an enzyme-linked immunosorbent assay (ELISA), we employed the proposed nanozyme to label the detecting antibody. According to the catalytic properties of the nanozyme, 4-nitrothiophenol (4-NTP) was introduced as the substrate, which can be transformed to 4-aminothiophenol (4-ATP) in the presence of NaBH4. In a surface enhanced Raman scattering (SERS) assay, 4-ATP was capable of functioning as a powerful bridge to connect the gold nanostars (with excellent SERS performance) by both the Au-S bond and electrostatic force to further produce a Raman "hot spot". Meanwhile, the Raman signal of 4-nitrothiophenol at 1573 cm(-1) was weakened, and a new signal at 1591 cm(-1) generated by 4-ATP was turned on, leading to the generation of a ratiometric SERS signal. Based on this performance, a ratiometric nanozyme-linked immunosorbent assay (NELISA) strategy was developed delicately, which was applied to detect beta-lactoglobulin (allergenic protein) by monitoring the ratiometric signal of I-1591/I-1573 with a limit of detection (LOD) of 0.01 ng/mL. The linear range is 25.65-6.2 x 10(4) ng/mL, covering more than 3 orders of magnitude. The developed method showed many advantages such as low-cost, higher recovery, and lower cross-reactivity, providing new insight into the application of SERS technology for trace target analysis. |
| 源URL | [http://210.75.249.4/handle/363003/59699]  |
| 专题 | 西北高原生物研究所_中国科学院西北高原生物研究所 |
| 推荐引用方式 GB/T 7714 | Su, Yiyun,Wu, Di,Chen, Jian,et al. Ratiometric Surface Enhanced Raman Scattering Immunosorbent Assay of Allergenic Proteins via Covalent Organic Framework Composite Material Based Nanozyme Tag Triggered Raman Signal "Turn-on" and Amplification[J]. ANALYTICAL CHEMISTRY,2019,91(18). |
| APA | Su, Yiyun.,Wu, Di.,Chen, Jian.,Chen, Guang.,Hu, Na.,...&Wut, Yongning.(2019).Ratiometric Surface Enhanced Raman Scattering Immunosorbent Assay of Allergenic Proteins via Covalent Organic Framework Composite Material Based Nanozyme Tag Triggered Raman Signal "Turn-on" and Amplification.ANALYTICAL CHEMISTRY,91(18). |
| MLA | Su, Yiyun,et al."Ratiometric Surface Enhanced Raman Scattering Immunosorbent Assay of Allergenic Proteins via Covalent Organic Framework Composite Material Based Nanozyme Tag Triggered Raman Signal "Turn-on" and Amplification".ANALYTICAL CHEMISTRY 91.18(2019). |
入库方式： OAI收割
来源：西北高原生物研究所
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