A novel and sensitive fluorescent assay for artemisinin with graphene quantum dots based on inner filter effect
文献类型：期刊论文
| 作者 | Zhu, Shuyun; Yan, Xiaolu; Sun, Jing ; Zhao, Xian-En; Wang, Xiao
|
| 刊名 | TALANTA
 |
| 出版日期 | 2019 |
| 卷号 | 200 |
| 关键词 | Artemisinin Graphene quantum dots Fluorescent assay Inner filter effect Pharmaceutical analysis |
| 英文摘要 | The authors describe a novel method for the determination of artemisinin (ART) by using graphene quantum dots (GQDs) as the fluorescent probes. This method is based on the fact that ART can react with p-aminophenylboronic acid (p-ABA) to produce p-aminophenol (p-AP). While in the presence of tyrosinase (TYR), p-AP can be oxidized into 4-amino-1,2-benzoquinone, which effectively quenched the fluorescence of GQDs due to the inner filter effect (IFE). By making use of these reactions, a novel and sensitive fluorescent assay for ART has been developed. The calibration curve for the determination of ART is linear in the range of 0.1-5 mu M and 5-55 mu M with the detection limit of 33 nM, which is more sensitive than most of other methods. Some common coexisting substances including Ca2+, Na+, Mg2+, K+, PO43-, starch, lactose, dextrin, and magnesium stearat have negligible effects on the fluorescence intensity of GQDs-TYR-p-ABA system. Finally, the sensing system was successfully applied to the detection of the compound naphthoquine phosphate tablet samples with satisfactory recoveries. This IFE-based GQDs fluorescence sensing strategy is facile and sensitive for the determination of ART because neither the surface modification nor the linking between the receptor and the fluorophore is required. |
| 源URL | [http://210.75.249.4/handle/363003/59739]  |
| 专题 | 西北高原生物研究所_中国科学院西北高原生物研究所 |
| 推荐引用方式 GB/T 7714 | Zhu, Shuyun,Yan, Xiaolu,Sun, Jing,et al. A novel and sensitive fluorescent assay for artemisinin with graphene quantum dots based on inner filter effect[J]. TALANTA,2019,200. |
| APA | Zhu, Shuyun,Yan, Xiaolu,Sun, Jing,Zhao, Xian-En,&Wang, Xiao.(2019).A novel and sensitive fluorescent assay for artemisinin with graphene quantum dots based on inner filter effect.TALANTA,200. |
| MLA | Zhu, Shuyun,et al."A novel and sensitive fluorescent assay for artemisinin with graphene quantum dots based on inner filter effect".TALANTA 200(2019). |
入库方式： OAI收割
来源：西北高原生物研究所
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