A practicable strategy for enrichment and separation of four minor flavonoids including two isomers from barley seedlings by macroporous resin column chromatography, medium-pressure LC, and high-speed countercurrent chromatography
文献类型：期刊论文
| 作者 | Wang, Nana; Chen, Tao; Yang, Xue; Shen, Cheng; Li, Hongmei; Wang, Shuo; Zhao, Jingyang; Chen, Jilin; Chen, Zhi; Li, Yulin
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| 刊名 | JOURNAL OF SEPARATION SCIENCE
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| 出版日期 | 2019 |
| 卷号 | 42期号:9 |
| 关键词 | barley seedlings high-speed counter-current chromatography isomers minor flavonoids |
| 英文摘要 | Separation of minor compounds especially with similar polarities and structures from complex samples is a challenging work. In the present study, an efficient method was successfully established by macroporous resin column chromatography, medium-pressure liquid chromatography, and high-speed countercurrent chromatography for separation of four minor flavonoids from barley seedlings. Macroporous resin column chromatography and medium-pressure liquid chromatography were used for enrichment of these four flavonoids. High-pressure liquid chromatography analysis showed the total content of these four flavonoids increased from 2.2% in the crude extract to 95.3% in the medium-pressure liquid chromatography fraction. It was indicated that the combination of macroporous resin column chromatography and medium-pressure liquid chromatography could be a practicable strategy for enrichment of minor compounds from complex sample. Then, high-speed countercurrent chromatography was employed for separation of these four flavonoids using ethyl acetate/n-butanol/water (0.1% glacial acetic acid) (4: 1: 5, v/v/v) as solvent system. As a result, four flavonoids including two isomers with purities higher than 98% were obtained. Interestingly, two flavonoids existing in one high-pressure liquid chromatography peak were also successfully separated. All these indicated high-speed countercurrent chromatography had great potential for separation of compounds with similar structures and polarities. This study provides a reference for efficient enrichment and separation of minor compounds from complex sample. |
| 源URL | [http://210.75.249.4/handle/363003/59796]  |
| 专题 | 西北高原生物研究所_中国科学院西北高原生物研究所 |
| 推荐引用方式 GB/T 7714 | Wang, Nana,Chen, Tao,Yang, Xue,et al. A practicable strategy for enrichment and separation of four minor flavonoids including two isomers from barley seedlings by macroporous resin column chromatography, medium-pressure LC, and high-speed countercurrent chromatography[J]. JOURNAL OF SEPARATION SCIENCE,2019,42(9). |
| APA | Wang, Nana.,Chen, Tao.,Yang, Xue.,Shen, Cheng.,Li, Hongmei.,...&Li, Yulin.(2019).A practicable strategy for enrichment and separation of four minor flavonoids including two isomers from barley seedlings by macroporous resin column chromatography, medium-pressure LC, and high-speed countercurrent chromatography.JOURNAL OF SEPARATION SCIENCE,42(9). |
| MLA | Wang, Nana,et al."A practicable strategy for enrichment and separation of four minor flavonoids including two isomers from barley seedlings by macroporous resin column chromatography, medium-pressure LC, and high-speed countercurrent chromatography".JOURNAL OF SEPARATION SCIENCE 42.9(2019). |
入库方式： OAI收割
来源：西北高原生物研究所
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