Triterpenic Acids from Potentilla parvifolia and Their Protective Effects against Okadaic Acid Induced Neurotoxicity in Differentiated SH-SY5Y Cells
文献类型：期刊论文
| 作者 | Yuan, Zhen-zhen; Suo, You-rui ; Hao, Xue-yan; Wang, Shu-lin; Li, Gang; Wang, Hong-lun
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| 刊名 | BIOLOGICAL & PHARMACEUTICAL BULLETIN
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| 出版日期 | 2018 |
| 卷号 | 41期号:6 |
| 关键词 | Potentilla parvifolia triterpenic acid SH-SY5Y cell tau phosphorylation reactive oxygen species |
| 英文摘要 | Six triterpenic acids were separated and purified from the ethyl acetate extractive fraction of ethanol extracts of Potentilla parvifolia Fiscx. using a variety of chromatographic methods. The neuroprotective effects of these triterpenoids were investigated in the present study, in which the okadaic acid induced neurotoxicity in human neuroblastoma SH-SY5Y cells were used as an Alzheimer's disease cell model in vitro. The cell model was established with all trans-retinoic acid (5pmol/L, 4d) and okadaic acid (40 nmol/L, 6h) treatments to induce tau phosphorylation and synaptic atrophy. Subsequently, the neuroprotective effects of these triterpenic acids were evaluated in vitro by this cell model. Results from the Western blot and morphology analysis suggested that compounds 3-6 had the better neuroprotective effects. Furthermore, we tested the level of mitochondrial reactive oxygen species and mitochondrial membrane potential of these compounds in SH-SY5Y cells by flow cytometry technology to investigate the potential neuroprotective mechanism of these compounds. All of the results indicated that maybe the mechanism of compounds 5 and 6 is to protect the cell from mitochondrial oxidative stress injuries. |
| 源URL | [http://210.75.249.4/handle/363003/60015]  |
| 专题 | 西北高原生物研究所_中国科学院西北高原生物研究所 |
| 推荐引用方式 GB/T 7714 | Yuan, Zhen-zhen,Suo, You-rui,Hao, Xue-yan,et al. Triterpenic Acids from Potentilla parvifolia and Their Protective Effects against Okadaic Acid Induced Neurotoxicity in Differentiated SH-SY5Y Cells[J]. BIOLOGICAL & PHARMACEUTICAL BULLETIN,2018,41(6). |
| APA | Yuan, Zhen-zhen,Suo, You-rui,Hao, Xue-yan,Wang, Shu-lin,Li, Gang,&Wang, Hong-lun.(2018).Triterpenic Acids from Potentilla parvifolia and Their Protective Effects against Okadaic Acid Induced Neurotoxicity in Differentiated SH-SY5Y Cells.BIOLOGICAL & PHARMACEUTICAL BULLETIN,41(6). |
| MLA | Yuan, Zhen-zhen,et al."Triterpenic Acids from Potentilla parvifolia and Their Protective Effects against Okadaic Acid Induced Neurotoxicity in Differentiated SH-SY5Y Cells".BIOLOGICAL & PHARMACEUTICAL BULLETIN 41.6(2018). |
入库方式： OAI收割
来源：西北高原生物研究所
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