Molecular Sex Identification in Dioecious Hippophae rhamnoides L. via RAPD and SCAR Markers
文献类型：期刊论文
| 作者 | Zhou, Wu; Wang, Yuwei; Zhang, Gong; Luan, Guangxiang; Chen, Shasha; Meng, Jing; Wang, Honglun ; Hu, Na; Suo, Yourui
|
| 刊名 | MOLECULES
 |
| 出版日期 | 2018 |
| 卷号 | 23期号:5 |
| 关键词 | Hippophae rhamnoides L dioecious sex determination RAPD SCAR |
| 英文摘要 | The dioecious property of the sea buckthorn (Hippophae rhamnoides L.) prevents sex recognition via traditional observation at the juvenile stage, thus impeding breeding and economic cropping; A random amplified polymorphic DNA (RAPD) and a sequence characterized amplified region (SCAR) markers were used to identify the sexes. A total of 45 random decamer primers were used to screen genomic DNA pools of staminate and pistillate genotypes for genetic polymorphisms. One female sex-linked marker was identified. D15 (5-CATCCGTGCT-3) amplified a particular band of 885 bp, which showed polymorphism among staminate and pistillate genotype plants. The SCAR marker Hrcx-15 was obtained by sequencing the fragment. The alleles of 140 pistillate genotypes were examined but not of the 140 staminate genotypes discerned via taxonomy. Staminate and pistillate genotypes of sea buckthorn plants can be distinguished, using Hrcx-15 as a genetic marker for sex identification and for expediting cultivation for commercial applications. |
| 源URL | [http://210.75.249.4/handle/363003/60017]  |
| 专题 | 西北高原生物研究所_中国科学院西北高原生物研究所 |
| 推荐引用方式 GB/T 7714 | Zhou, Wu,Wang, Yuwei,Zhang, Gong,et al. Molecular Sex Identification in Dioecious Hippophae rhamnoides L. via RAPD and SCAR Markers[J]. MOLECULES,2018,23(5). |
| APA | Zhou, Wu.,Wang, Yuwei.,Zhang, Gong.,Luan, Guangxiang.,Chen, Shasha.,...&Suo, Yourui.(2018).Molecular Sex Identification in Dioecious Hippophae rhamnoides L. via RAPD and SCAR Markers.MOLECULES,23(5). |
| MLA | Zhou, Wu,et al."Molecular Sex Identification in Dioecious Hippophae rhamnoides L. via RAPD and SCAR Markers".MOLECULES 23.5(2018). |
入库方式： OAI收割
来源：西北高原生物研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。