Clioquinol induces G(2)/M cell cycle arrest through the up-regulation of TDH3 in Saccharomyces cerevisiae
文献类型：期刊论文
| 作者 | Yan, Chongjia; Wang, Song; Wang, Jian; Li, Hui; Huang, Zhiwei; Sun, Jing ; Peng, Min; Liu, Wenbin; Shi, Ping
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| 刊名 | MICROBIOLOGICAL RESEARCH
 |
| 出版日期 | 2018 |
| 卷号 | 214 |
| 关键词 | Saccharomyces cerevisiae Clioquinol Cell cycle arrest Proteomics TDH3 |
| 英文摘要 | Clioquinol (CQ) has been used as a classical antimicrobial agent for many years. However, its mode of action is still unclear. In our study, the growth of Candida albicans and Saccharomyces cerevisiae was inhibited by CQ. It did not kill yeast cells, but shortened G1 phase and arrested cell cycle at G(2)/M phase. By using two-dimensional electrophoresis based proteomic approach, six proteins were found to be significantly affected by CQ. Among them, four (PDC1, ADH1, TDH3, IPP1) were up-regulated and the other two (TDH1 and PGK1) were downregulated. According to the Saccharomyces Genome Database (SGD), these proteins were involved in various biological processes including glycolytic fermentation, gluconeogenesis, glycolytic process, amino acid catabolism, redox reaction and reactive oxygen species metabolic process. It was noted that there was a link between TDH3 and cell cycle. The overexpression of TDH3 phenocopied CQ treatment and arrested the cell cycle at G(2)/M phase. RT-PCR analysis showed that the mRNA levels of CLN3 and CDC28, critical genes for passage through G1 phase, were up-regulated after the treatment of CQ as well as the overexpression of TDH3. It demonstrates that CQ inhibits the growth of yeast by up-regulating the expression of TDH3 to influence the cell cycle. It is expected to provide new insights for the antimicrobial mechanism of CQ. |
| 源URL | [http://210.75.249.4/handle/363003/60031]  |
| 专题 | 西北高原生物研究所_中国科学院西北高原生物研究所 |
| 推荐引用方式 GB/T 7714 | Yan, Chongjia,Wang, Song,Wang, Jian,et al. Clioquinol induces G(2)/M cell cycle arrest through the up-regulation of TDH3 in Saccharomyces cerevisiae[J]. MICROBIOLOGICAL RESEARCH,2018,214. |
| APA | Yan, Chongjia.,Wang, Song.,Wang, Jian.,Li, Hui.,Huang, Zhiwei.,...&Shi, Ping.(2018).Clioquinol induces G(2)/M cell cycle arrest through the up-regulation of TDH3 in Saccharomyces cerevisiae.MICROBIOLOGICAL RESEARCH,214. |
| MLA | Yan, Chongjia,et al."Clioquinol induces G(2)/M cell cycle arrest through the up-regulation of TDH3 in Saccharomyces cerevisiae".MICROBIOLOGICAL RESEARCH 214(2018). |
入库方式： OAI收割
来源：西北高原生物研究所
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