PfHMGB2 protects yellow catfish (Pelteobagrus fulvidraco) from bacterial infection by promoting phagocytosis and proliferation of PBL
文献类型：期刊论文
| 作者 | Wang, Yun1,2; Yang, Yanyan2; Chen, Qianying2; Zhai, Hanfei2; Xie, Zhaohui2; Ke, Fei3 |
| 刊名 | FISH & SHELLFISH IMMUNOLOGY
 |
| 出版日期 | 2019-10-01 |
| 卷号 | 93页码:567-574 |
| 关键词 | HMGB2 DNA affinity Antibacterial activity Phagocytosis Proliferation TNF alpha Yellow catfish |
| ISSN号 | 1050-4648 |
| DOI | 10.1016/j.fsi.2019.08.007 |
| 通讯作者 | Wang, Yun(y-wang401@163.com) |
| 英文摘要 | HMGB2, a member of the high mobility group box family, plays an important role in host immune responses. However, the mechanism of action of HMGB2 is not well understood. Herein, a homologue from yellow catfish (Pelteobagrus fulvidraco) was cloned and named PfHMGB2. The deduced amino acid sequence of PfHMGB2 possessed a typical tripartite structure (two DNA binding boxes and an acid tail) and shared 90% identity with the predicted HMGB2 from I. punctatus. The mRNA of PfHMGB2 was widely distributed in all 11 tested tissues in healthy fish bodies and was significantly induced in the liver and head kidney when yellow catfish were injected with inactivated Aeromonas hydrophila. Consistently, PfHMGB2 mRNA could also be induced in yellow catfish peripheral blood leucocytes (PBL) by lipopolysaccharide. The recombinant PfHMGB2 protein was purified from E. coli BL21 (DE3):pET-28a/PfHMGB2 and showed DNA-binding affinity. Moreover, rPfHMGB2 improved the phagocytosis and proliferation activity and upregulated the mRNA expression of the pro-inflammatory cytokine TNFa in yellow catfish PBL. These results indicated that PfHMGB2 could protect yellow catfish from pathogen infection by activating PBL. |
| WOS关键词 | GROUP BOX 1 ; MOBILITY GROUP B2 ; HMGB PROTEINS ; DNA-BINDING ; CYSTEINE RESIDUES ; GENE ; CLONING ; MICE ; IDENTIFICATION ; MOLECULE |
| 资助项目 | National Natural Science Foundation of China[31702388] |
| WOS研究方向 | Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences |
| 语种 | 英语 |
| WOS记录号 | WOS:000488998300063 |
| 出版者 | ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD |
| 资助机构 | National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China |
| 源URL | [http://ir.ihb.ac.cn/handle/342005/34376]  |
| 专题 | 水生生物研究所_其他_期刊论文 |
| 通讯作者 | Wang, Yun |
| 作者单位 | 1.Jianghan Univ, Inst Environm & Hlth, Hubei Key Lab Environm & Hlth Effects Persistent, Wuhan 430056, Hubei, Peoples R China 2.Henan Univ Urban Construct, Henan Prov Key Lab Water Pollut Control & Rehabil, Pingdingshan 467036, Henan, Peoples R China 3.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Yun,Yang, Yanyan,Chen, Qianying,et al. PfHMGB2 protects yellow catfish (Pelteobagrus fulvidraco) from bacterial infection by promoting phagocytosis and proliferation of PBL[J]. FISH & SHELLFISH IMMUNOLOGY,2019,93:567-574. |
| APA | Wang, Yun,Yang, Yanyan,Chen, Qianying,Zhai, Hanfei,Xie, Zhaohui,&Ke, Fei.(2019).PfHMGB2 protects yellow catfish (Pelteobagrus fulvidraco) from bacterial infection by promoting phagocytosis and proliferation of PBL.FISH & SHELLFISH IMMUNOLOGY,93,567-574. |
| MLA | Wang, Yun,et al."PfHMGB2 protects yellow catfish (Pelteobagrus fulvidraco) from bacterial infection by promoting phagocytosis and proliferation of PBL".FISH & SHELLFISH IMMUNOLOGY 93(2019):567-574. |
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来源：水生生物研究所
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