Agar extraction from Pyropia haitanensis residue after the removal and purification of phycobiliproteins
文献类型：期刊论文
| 作者 | Zhao, Ping1,2,3,4; Niu, Jianfeng1,2,4; Huan, Li1,2,4 ; Gu, Wenhui1,2,4; Wu, Mingjiang5; Wang, Guangce1,2,4
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| 刊名 | JOURNAL OF APPLIED PHYCOLOGY
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| 出版日期 | 2019-08-01 |
| 卷号 | 31期号:4页码:2497-2505 |
| 关键词 | Pyropia haitanensis Expanded bed adsorption R-phycoerythrin Agar |
| ISSN号 | 0921-8971 |
| DOI | 10.1007/s10811-019-1735-z |
| 通讯作者 | Wang, Guangce(gcwang@qdio.ac.cn) |
| 英文摘要 | Pyropia haitanensis is one of the most important and commercial species of edible seaweed in China. However, at its late growth stages it is unsuitable for food processing and is usually treated as waste. The present study investigated the full use of aging Py. haitanensis and attempted to improve its economic value through the purification of R-phycoerythrin combining the extraction of agar from the dry residue after phycobiliprotein extraction. The recovery of R-phycoerythrin from crude extract was 12.12% with a purification ratio of > 4. The agar yield was 5.81% with a gel strength of 796.00 +/- 28.10 g cm(-2), a gelling temperature of 38.10 +/- 0.22 degrees C, a melting temperature of 89.50 +/- 0.55 degrees C, 3,6-anhydrogalactose content of 34% +/- 3.16%, and sulfate content of 3.01% +/- 0.24%. There were no significant differences between these parameters and those from the direct extraction of Py. haitanensis. The agar extracted exhibited some physicochemical properties that have industrial applications. It could also be used as a material for DNA electrophoresis and a bacterial culture medium. This new method of comprehensive extraction reduced use of water and alkali, and waste liquid output, which were 36.52 L, 0.40 L, and 39.15 L, respectively, less than that from direct Py. haitanensis extraction. Total nitrogen, suspended solids, phosphorus, ammonia-nitrogen, chemical oxygen demand, and biochemical oxygen demand of the extract after 5 days were 38.37 mg, 4.72 x 10(3) mg, 62.82 mg, 18.32 mg, 8.46 x 10(3) mg, and 2.21 x 10(3) mg, respectively, less than that directly from Py. haitanensis. |
| 资助项目 | National Key Research and Development Program of China[2016YFC1400606] ; Natural Science Foundation of China[31470430] ; Natural Science Foundation of China[41876197] |
| WOS研究方向 | Biotechnology & Applied Microbiology ; Marine & Freshwater Biology |
| 语种 | 英语 |
| WOS记录号 | WOS:000479057200030 |
| 出版者 | SPRINGER |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/162363]  |
| 专题 | 海洋研究所_实验海洋生物学重点实验室 |
| 通讯作者 | Wang, Guangce |
| 作者单位 | 1.Chinese Acad Sci, Key Lab Expt Marine Biol, Inst Oceanol, Qingdao 266071, Shandong, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266237, Shandong, Peoples R China 3.Univ Chinese Acad Sci, Coll Earth Sci, Beijing 100049, Peoples R China 4.Chinese Acad Sci, Ctr Ocean Mega Sci, 7 Nanhai Rd, Qingdao 266071, Shandong, Peoples R China 5.Wenzhou Univ, Coll Life & Environm Sci, Wenzhou, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhao, Ping,Niu, Jianfeng,Huan, Li,et al. Agar extraction from Pyropia haitanensis residue after the removal and purification of phycobiliproteins[J]. JOURNAL OF APPLIED PHYCOLOGY,2019,31(4):2497-2505. |
| APA | Zhao, Ping,Niu, Jianfeng,Huan, Li,Gu, Wenhui,Wu, Mingjiang,&Wang, Guangce.(2019).Agar extraction from Pyropia haitanensis residue after the removal and purification of phycobiliproteins.JOURNAL OF APPLIED PHYCOLOGY,31(4),2497-2505. |
| MLA | Zhao, Ping,et al."Agar extraction from Pyropia haitanensis residue after the removal and purification of phycobiliproteins".JOURNAL OF APPLIED PHYCOLOGY 31.4(2019):2497-2505. |
入库方式： OAI收割
来源：海洋研究所
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