Pr³⁺:CaTiO₃, a robust persistent luminescent material, was coupled with chiral molecules and Ag nanoparticles (NPs) to construct a Pr³⁺:CaTiO₃@Ag@L-cysteine ternary material that can realize rapid enantiomer selective sensing of L- and D-arginine by making use of the chiral induced spin selectivity (CISS) effect. In addition, long-lifetime photoelectrons excited in the Pr³⁺:CaTiO₃ matrix were effectively transported through a Ag NP “bridge” into the L-cysteine chiral monolayer, due to cooperation between each component in the ternary material.