Preparation and evaluation of hydrophobically associating polyacrylamide coated silica composite as high performance liquid chromatographic stationary phase
文献类型:期刊论文
作者 | XiaojingLiang2; ShuaiWang2; YongGuo2; LichengWang2; XiaoSong1; TiantianSi2,3; Licheng Wang; Xiaojing Liang; Shuai Wang; Shuai Wang |
刊名 | Microchemistry Journal |
出版日期 | 2020 |
卷号 | 152期号:1页码:104330 |
关键词 | Hydrophilic interaction liquid chromatography Hydrophilic polymer Hydrophobically associating polyacrylamide stationary phase |
DOI | 10.1016/j.microc.2019.104330 |
英文摘要 | A hydrophobically associating polyacrylamide (HAPM)-coated silica packing (HAPM@silica) as a novel stationary phase was synthesized by a simple thermal immobilization to separate multiple polar compounds for the first time. The successful immobilization of HAPM on the silica support was confirmed by elemental analysis, scanning electron microscopy (SEM) and the Brunauer–Emmett–Teller (BET). The thickness of HAPM coating is crucial and the one-layer HAPM@silica stationary phase was demonstrated the highest separation efficiency. Compared with the bare silica and amino modified silica, the new stationary phase showed better separation performance for multiple polar compounds such as bases, alkaloids, carbohydrates and amino acid compounds under the experimental conditions. The chromatographic behaviors were evaluated by investigating the effects of acetonitrile content, water content, mobile phase pH on separation. A typical hydrophilic interaction liquid chromatography (HILIC) retention feature of HAPM based column was observed at the low percentage of water content. The packing material also showed excellent chromatographic repeatability with intraday RSDs of the retention time of five bases less than 0.46 % (n = 10) and interday RSDs less than 1.45% (n = 5). In summary, the new chromatographic material can be used for a feasible option for analysis of polar compounds. |
语种 | 英语 |
源URL | [http://210.77.64.217/handle/362003/25739] |
专题 | 兰州化学物理研究所_中科院西北特色植物资源化学重点实验室/甘肃省天然药物重点实验室 |
通讯作者 | XiaojingLiang; ShuaiWang; Xiaojing Liang; Shuai Wang; Shuai Wang |
作者单位 | 1.3Engineering Technology Research Institute of Xinjiang Oilfied Company Petrochina, Karamay 834000, China 2.1CAS Key Laboratory of Chemistry of Northwestern Plant Resources and Key Laboratory for Natural Medicine of Gansu Province, Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences, Lanzhou 730000, China 3.2University of Chinese Academy of Sciences, Beijing 100049, China |
推荐引用方式 GB/T 7714 | XiaojingLiang,ShuaiWang,YongGuo,et al. Preparation and evaluation of hydrophobically associating polyacrylamide coated silica composite as high performance liquid chromatographic stationary phase[J]. Microchemistry Journal,2020,152(1):104330. |
APA | XiaojingLiang.,ShuaiWang.,YongGuo.,LichengWang.,XiaoSong.,...&Yong Guo.(2020).Preparation and evaluation of hydrophobically associating polyacrylamide coated silica composite as high performance liquid chromatographic stationary phase.Microchemistry Journal,152(1),104330. |
MLA | XiaojingLiang,et al."Preparation and evaluation of hydrophobically associating polyacrylamide coated silica composite as high performance liquid chromatographic stationary phase".Microchemistry Journal 152.1(2020):104330. |
入库方式: OAI收割
来源:兰州化学物理研究所
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