The Accuracy of Survival Time Prediction for Patients with Glioma Is Improved by Measuring Mitotic Spindle Checkpoint Gene Expression
文献类型：期刊论文
| 作者 | Bie, Li1,2; Zhao, Gang1; Cheng, Pui3; Rondeau, Gaelle3; Porwollik, Steffen3; Ju, Yan1; Xia, Xiao-Qin2,3,4; McClelland, Michael2,3 |
| 刊名 | PLOS ONE
 |
| 出版日期 | 2011-10-12 |
| 卷号 | 6期号:10页码:e25631 |
| 关键词 | CENTRAL-NERVOUS-SYSTEM CHROMOSOMAL INSTABILITY BREAST-CANCER CELL-PROLIFERATION BLADDER-CANCER HUMAN ASTROCYTOMAS GASTRIC-CANCER BRAIN-TUMORS RT-PCR GLIOBLASTOMA |
| ISSN号 | 1932-6203 |
| 通讯作者 | Bie, L (reprint author), Jilin Univ, Clin Hosp 1, Dept Neurosurg, Changchun 130023, Peoples R China ; xqxia@ihb.ac.cn ; mmcclell@uci.edu |
| 中文摘要 | Identification of gene expression changes that improve prediction of survival time across all glioma grades would be clinically useful. Four Affymetrix GeneChip datasets from the literature, containing data from 771 glioma samples representing all WHO grades and eight normal brain samples, were used in an ANOVA model to screen for transcript changes that correlated with grade. Observations were confirmed and extended using qPCR assays on RNA derived from 38 additional glioma samples and eight normal samples for which survival data were available. RNA levels of eight major mitotic spindle assembly checkpoint (SAC) genes (BUB1, BUB1B, BUB3, CENPE, MAD1L1, MAD2L1, CDC20, TTK) significantly correlated with glioma grade and six also significantly correlated with survival time. In particular, the level of BUB1B expression was highly correlated with survival time (p<0.0001), and significantly outperformed all other measured parameters, including two standards; WHO grade and MIB-1 (Ki-67) labeling index. Measurement of the expression levels of a small set of SAC genes may complement histological grade and other clinical parameters for predicting survival time. |
| 英文摘要 | Identification of gene expression changes that improve prediction of survival time across all glioma grades would be clinically useful. Four Affymetrix GeneChip datasets from the literature, containing data from 771 glioma samples representing all WHO grades and eight normal brain samples, were used in an ANOVA model to screen for transcript changes that correlated with grade. Observations were confirmed and extended using qPCR assays on RNA derived from 38 additional glioma samples and eight normal samples for which survival data were available. RNA levels of eight major mitotic spindle assembly checkpoint (SAC) genes (BUB1, BUB1B, BUB3, CENPE, MAD1L1, MAD2L1, CDC20, TTK) significantly correlated with glioma grade and six also significantly correlated with survival time. In particular, the level of BUB1B expression was highly correlated with survival time (p<0.0001), and significantly outperformed all other measured parameters, including two standards; WHO grade and MIB-1 (Ki-67) labeling index. Measurement of the expression levels of a small set of SAC genes may complement histological grade and other clinical parameters for predicting survival time. |
| WOS标题词 | Science & Technology |
| 学科主题 | Life Sciences & Biomedicine - Other Topics |
| 类目[WOS] | Multidisciplinary Sciences |
| 研究领域[WOS] | Science & Technology - Other Topics |
| 关键词[WOS] | CENTRAL-NERVOUS-SYSTEM ; CHROMOSOMAL INSTABILITY ; BREAST-CANCER ; CELL-PROLIFERATION ; BLADDER-CANCER ; HUMAN ASTROCYTOMAS ; GASTRIC-CANCER ; BRAIN-TUMORS ; RT-PCR ; GLIOBLASTOMA |
| 收录类别 | SCI |
| 资助信息 | National Cancer Institute Early Detection Research Network[U01CA152738]; CDMRP BCRP[W81XWH-08-1-0720]; State Scholarship Fund from China[2009104187]; Jilin University |
| 语种 | 英语 |
| WOS记录号 | WOS:000295976000026 |
| 公开日期 | 2011-12-22 |
| 源URL | [http://ir.ihb.ac.cn/handle/342005/16469]  |
| 专题 | 水生生物研究所_水生生物分子与细胞生物学研究中心_期刊论文 |
| 作者单位 | 1.Jilin Univ, Clin Hosp 1, Dept Neurosurg, Changchun 130023, Peoples R China 2.Univ Calif Irvine, Dept Pathol & Lab Med, Irvine, CA USA 3.Vaccine Res Inst San Diego, San Diego, CA USA 4.Chinese Acad Sci, Inst Hydrobiol, Wuhan, Peoples R China |
| 推荐引用方式 GB/T 7714 | Bie, Li,Zhao, Gang,Cheng, Pui,et al. The Accuracy of Survival Time Prediction for Patients with Glioma Is Improved by Measuring Mitotic Spindle Checkpoint Gene Expression[J]. PLOS ONE,2011,6(10):e25631. |
| APA | Bie, Li.,Zhao, Gang.,Cheng, Pui.,Rondeau, Gaelle.,Porwollik, Steffen.,...&McClelland, Michael.(2011).The Accuracy of Survival Time Prediction for Patients with Glioma Is Improved by Measuring Mitotic Spindle Checkpoint Gene Expression.PLOS ONE,6(10),e25631. |
| MLA | Bie, Li,et al."The Accuracy of Survival Time Prediction for Patients with Glioma Is Improved by Measuring Mitotic Spindle Checkpoint Gene Expression".PLOS ONE 6.10(2011):e25631. |
入库方式： OAI收割
来源：水生生物研究所
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