Zebrafish foxo3b Negatively Regulates Canonical Wnt Signaling to Affect Early Embryogenesis
文献类型：期刊论文
| 作者 | Xie, Xun-wei; Liu, Jing-Xia; Hu, Bo; Xiao, Wuhan |
| 刊名 | PLOS ONE
 |
| 出版日期 | 2011-09-07 |
| 卷号 | 6期号:9页码:e24469 |
| 关键词 | FORKHEAD TRANSCRIPTION FACTOR POSTERIOR MESODERM FORMATION BETA-CATENIN HOMEOBOX GENE ORGANIZER FORMATION XENOPUS MESODERM DORSAL ORGANIZER EXPRESSION GASTRULATION PROTEINS |
| ISSN号 | 1932-6203 |
| 通讯作者 | Xie, XW (reprint author), Chinese Acad Sci, Inst Hydrobiol, Key Lab Aquat Biodivers & Conservat, Wuhan, Peoples R China ; w-xiao@ihb.ac.cn |
| 中文摘要 | FOXO genes are involved in many aspects of development and vascular homeostasis by regulating cell apoptosis, proliferation, and the control of oxidative stress. In addition, FOXO genes have been showed to inhibit Wnt/beta-catenin signaling by competing with T cell factor to bind to beta-catenin. However, how important of this inhibition in vivo, particularly in embryogenesis is still unknown. To demonstrate the roles of FOXO genes in embryogenesis will help us to further understand their relevant physiological functions. Zebrafish foxo3b gene, an orthologue of mammalian FOXO3, was expressed maternally and distributed ubiquitously during early embryogenesis and later restricted to brain. After morpholino-mediated knockdown of foxo3b, the zebrafish embryos exhibited defects in axis and neuroectoderm formation, suggesting its critical role in early embryogenesis. The embryo-developmental marker gene staining at different stages, phenotype analysis and rescue assays revealed that foxo3b acted its role through negatively regulating both maternal and zygotic Wnt/beta-catenin signaling. Moreover, we found that foxo3b could interact with zebrafish beta-catenin1 and beta-catenin2 to suppress their transactivation in vitro and in vivo, further confirming its role relevant to the inhibition of Wnt/beta-catenin signaling. Taken together, we revealed that foxo3b played a very important role in embryogenesis and negatively regulated maternal and zygotic Wnt/beta-catenin signaling by directly interacting with both beta-catenin1 and beta-catenin2. Our studies provide an in vivo model for illustrating function of FOXO transcription factors in embryogenesis. |
| 英文摘要 | FOXO genes are involved in many aspects of development and vascular homeostasis by regulating cell apoptosis, proliferation, and the control of oxidative stress. In addition, FOXO genes have been showed to inhibit Wnt/beta-catenin signaling by competing with T cell factor to bind to beta-catenin. However, how important of this inhibition in vivo, particularly in embryogenesis is still unknown. To demonstrate the roles of FOXO genes in embryogenesis will help us to further understand their relevant physiological functions. Zebrafish foxo3b gene, an orthologue of mammalian FOXO3, was expressed maternally and distributed ubiquitously during early embryogenesis and later restricted to brain. After morpholino-mediated knockdown of foxo3b, the zebrafish embryos exhibited defects in axis and neuroectoderm formation, suggesting its critical role in early embryogenesis. The embryo-developmental marker gene staining at different stages, phenotype analysis and rescue assays revealed that foxo3b acted its role through negatively regulating both maternal and zygotic Wnt/beta-catenin signaling. Moreover, we found that foxo3b could interact with zebrafish beta-catenin1 and beta-catenin2 to suppress their transactivation in vitro and in vivo, further confirming its role relevant to the inhibition of Wnt/beta-catenin signaling. Taken together, we revealed that foxo3b played a very important role in embryogenesis and negatively regulated maternal and zygotic Wnt/beta-catenin signaling by directly interacting with both beta-catenin1 and beta-catenin2. Our studies provide an in vivo model for illustrating function of FOXO transcription factors in embryogenesis. |
| WOS标题词 | Science & Technology |
| 学科主题 | Life Sciences & Biomedicine - Other Topics |
| 类目[WOS] | Multidisciplinary Sciences |
| 研究领域[WOS] | Science & Technology - Other Topics |
| 关键词[WOS] | FORKHEAD TRANSCRIPTION FACTOR ; POSTERIOR MESODERM FORMATION ; BETA-CATENIN ; HOMEOBOX GENE ; ORGANIZER FORMATION ; XENOPUS MESODERM ; DORSAL ORGANIZER ; EXPRESSION ; PROTEINS ; PATHWAYS |
| 收录类别 | SCI |
| 资助信息 | 973 grant[2010CB126306, 2007CB815705]; NSFC[91019008, 31071212, 30971667, 20890113]; National Transgene Project[2009ZX08010-021B]; Chinese Academy of Science[KSCX2-Y-W-N-020, KSCX2-EW-Q-12] |
| 语种 | 英语 |
| WOS记录号 | WOS:000294802500050 |
| 公开日期 | 2011-12-22 |
| 源URL | [http://ir.ihb.ac.cn/handle/342005/16471]  |
| 专题 | 水生生物研究所_水生生物分子与细胞生物学研究中心_期刊论文 |
| 作者单位 | Chinese Acad Sci, Inst Hydrobiol, Key Lab Aquat Biodivers & Conservat, Wuhan, Peoples R China |
| 推荐引用方式 GB/T 7714 | Xie, Xun-wei,Liu, Jing-Xia,Hu, Bo,et al. Zebrafish foxo3b Negatively Regulates Canonical Wnt Signaling to Affect Early Embryogenesis[J]. PLOS ONE,2011,6(9):e24469. |
| APA | Xie, Xun-wei,Liu, Jing-Xia,Hu, Bo,&Xiao, Wuhan.(2011).Zebrafish foxo3b Negatively Regulates Canonical Wnt Signaling to Affect Early Embryogenesis.PLOS ONE,6(9),e24469. |
| MLA | Xie, Xun-wei,et al."Zebrafish foxo3b Negatively Regulates Canonical Wnt Signaling to Affect Early Embryogenesis".PLOS ONE 6.9(2011):e24469. |
入库方式： OAI收割
来源：水生生物研究所
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