Centrifuge microextraction coupled with sweeping-MEKC to analyze trace steroid hormones in urine samples
文献类型：期刊论文
| 作者 | Fang, Huaifang1; Yang, Fangxing2; Sun, Junling1; Tian, Yun1; Zeng, Zhaorui1; Xu, Ying2 |
| 刊名 | TALANTA
 |
| 出版日期 | 2011-09-30 |
| 卷号 | 85期号:4页码:2148-2153 |
| 关键词 | Centrifuge microextraction Sweeping Micellar electrokinetic chromatography Steroid hormone |
| ISSN号 | 0039-9140 |
| 通讯作者 | Zeng, ZR (reprint author), Wuhan Univ, Dept Chem, Wuhan 430072, Peoples R China ; zrzeng@whu.edu.cn |
| 中文摘要 | A novel method that combines two concentration techniques, off-line centrifuge microextraction (CME) and on-line sweeping, is used to determine trace steroids in urine by micellar electrokinetic chromatography (MEKC). The CME-sweeping-MEKC is a promising technique, which has a variety of merits such as high sensitivity, rapid operation, minimal cost, and high sample throughput capability. Using CME-sweeping, over a 500-fold increase in sensitivity could be obtained as compared with the normal hydrodynamic injection without sample stacking. The linear range was 0.05-1 mu g mL(-1) with the square of the correlation coefficients ranging from 0.9998 to 0.9999. Detection limits (S/N = 3) were 5-15 ng mL(-1) using a photodiode array UV detection at a wavelength of 240 nm. The relative standard deviations were between 2.8% and 3.5% (n = 5, progesterone as the internal standard). The diode array UV spectrum used can distinguish the analytes from the interference in the complex sample matrix, which is useful in biological and clinical sample analysis. (C) 2011 Elsevier B.V. All rights reserved. |
| 英文摘要 | A novel method that combines two concentration techniques, off-line centrifuge microextraction (CME) and on-line sweeping, is used to determine trace steroids in urine by micellar electrokinetic chromatography (MEKC). The CME-sweeping-MEKC is a promising technique, which has a variety of merits such as high sensitivity, rapid operation, minimal cost, and high sample throughput capability. Using CME-sweeping, over a 500-fold increase in sensitivity could be obtained as compared with the normal hydrodynamic injection without sample stacking. The linear range was 0.05-1 mu g mL(-1) with the square of the correlation coefficients ranging from 0.9998 to 0.9999. Detection limits (S/N = 3) were 5-15 ng mL(-1) using a photodiode array UV detection at a wavelength of 240 nm. The relative standard deviations were between 2.8% and 3.5% (n = 5, progesterone as the internal standard). The diode array UV spectrum used can distinguish the analytes from the interference in the complex sample matrix, which is useful in biological and clinical sample analysis. (C) 2011 Elsevier B.V. All rights reserved. |
| WOS标题词 | Science & Technology ; Physical Sciences |
| 学科主题 | Chemistry |
| 类目[WOS] | Chemistry, Analytical |
| 研究领域[WOS] | Chemistry |
| 关键词[WOS] | MICELLAR ELECTROKINETIC CHROMATOGRAPHY ; DENSITY-GRADIENT ULTRACENTRIFUGATION ; PERFORMANCE LIQUID-CHROMATOGRAPHY ; REVERSE MIGRATING MICELLES ; IONIZATION MASS-SPECTROMETRY ; WALLED CARBON NANOTUBES ; METAL-ION COMPLEXES ; CAPILLARY-ELECTROPHORESIS ; ONLINE CONCENTRATION ; NEUTRAL ANALYTES |
| 收录类别 | SCI |
| 资助信息 | National Natural Science Foundation of China[20375028]; National Basic Research Program, China[2003CB415005]; Chinese Academy of Sciences[KSCX2-SW-128] |
| 语种 | 英语 |
| WOS记录号 | WOS:000295393000066 |
| 公开日期 | 2011-12-22 |
| 源URL | [http://ir.ihb.ac.cn/handle/342005/16457]  |
| 专题 | 水生生物研究所_水环境工程研究中心_期刊论文 |
| 作者单位 | 1.Wuhan Univ, Dept Chem, Wuhan 430072, Peoples R China 2.Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Peoples R China |
| 推荐引用方式 GB/T 7714 | Fang, Huaifang,Yang, Fangxing,Sun, Junling,et al. Centrifuge microextraction coupled with sweeping-MEKC to analyze trace steroid hormones in urine samples[J]. TALANTA,2011,85(4):2148-2153. |
| APA | Fang, Huaifang,Yang, Fangxing,Sun, Junling,Tian, Yun,Zeng, Zhaorui,&Xu, Ying.(2011).Centrifuge microextraction coupled with sweeping-MEKC to analyze trace steroid hormones in urine samples.TALANTA,85(4),2148-2153. |
| MLA | Fang, Huaifang,et al."Centrifuge microextraction coupled with sweeping-MEKC to analyze trace steroid hormones in urine samples".TALANTA 85.4(2011):2148-2153. |
入库方式： OAI收割
来源：水生生物研究所
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