Effects of xenoestrogens on the expression of vitellogenin (vtg) and cytochrome P450 aromatase (cyp19a and b) genes in zebrafish (Danio rerio) larvae
文献类型：期刊论文
| 作者 | Wang, Jingxian; Shi, Xiongjie; Du, Yongbin; Zhou, Bingsheng |
| 刊名 | JOURNAL OF ENVIRONMENTAL SCIENCE AND HEALTH PART A-TOXIC/HAZARDOUS SUBSTANCES & ENVIRONMENTAL ENGINEERING
 |
| 出版日期 | 2011 |
| 卷号 | 46期号:9页码:960-967 |
| 关键词 | Endocrine disrupter chemicals brominated flame retardant vitellogenin aromatase genes cyp19a/b zebrafish larvae |
| ISSN号 | 1093-4529 |
| 通讯作者 | Wang, JX (reprint author), Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan, Peoples R China ; wangjx@ihb.ac.cn |
| 中文摘要 | In the present study, expression levels of vitellogenin (vtg) and cytochrome P450 aromatase genes (cyp19a and cyp19b) in zebrafish larvae during the early stages of development were investigated by quantitative real time-PCR assay. The results indicated that vtg gene transcription was induced seven days after zebrafish larvae fertilization, whereas the expression of cyp19a and cyp19b genes was detected as early as 3 and 4 days post-fertilization (dpf). Investigations into the effects of 17 beta-estradiol (E2) exposure on the expression of these genes showed that both vtg and cyp19b were upregulated by E2 in zebrafish larvae as early as four dpf, whereas no variation was observed in cyp19a gene expression. The estrogenic potential of pharmaceutical estrogen (DES), phenol estrogen (BPA) and the brominated flame retardants, TBBPA, DE-71 and 4-BP, were evaluated by analyzing the expression of these three genes in zebrafish larvae. The results demonstrated that natural estrogen, endocrine disrupting compounds and brominated flame retardants act as endocrine disrupters through different mechanisms. We have demonstrated for the first time that the polybrominated diphenyl ether mixture, DE-71, acts as an endocrine disrupter by upregulation of cyp19b gene expression at a relatively low concentration. These results indicate that analysis of vtg and cyp19b gene expression in zebrafish during early embryogenesis and organogenesis represents the basis of a sensitive and fast bioassay for the routine assessment of xenoestrogen effects. |
| 英文摘要 | In the present study, expression levels of vitellogenin (vtg) and cytochrome P450 aromatase genes (cyp19a and cyp19b) in zebrafish larvae during the early stages of development were investigated by quantitative real time-PCR assay. The results indicated that vtg gene transcription was induced seven days after zebrafish larvae fertilization, whereas the expression of cyp19a and cyp19b genes was detected as early as 3 and 4 days post-fertilization (dpf). Investigations into the effects of 17 beta-estradiol (E2) exposure on the expression of these genes showed that both vtg and cyp19b were upregulated by E2 in zebrafish larvae as early as four dpf, whereas no variation was observed in cyp19a gene expression. The estrogenic potential of pharmaceutical estrogen (DES), phenol estrogen (BPA) and the brominated flame retardants, TBBPA, DE-71 and 4-BP, were evaluated by analyzing the expression of these three genes in zebrafish larvae. The results demonstrated that natural estrogen, endocrine disrupting compounds and brominated flame retardants act as endocrine disrupters through different mechanisms. We have demonstrated for the first time that the polybrominated diphenyl ether mixture, DE-71, acts as an endocrine disrupter by upregulation of cyp19b gene expression at a relatively low concentration. These results indicate that analysis of vtg and cyp19b gene expression in zebrafish during early embryogenesis and organogenesis represents the basis of a sensitive and fast bioassay for the routine assessment of xenoestrogen effects. |
| WOS标题词 | Science & Technology ; Technology ; Life Sciences & Biomedicine |
| 学科主题 | Engineering; Environmental Sciences & Ecology |
| 类目[WOS] | Engineering, Environmental ; Environmental Sciences |
| 研究领域[WOS] | Engineering ; Environmental Sciences & Ecology |
| 关键词[WOS] | BROMINATED FLAME RETARDANTS ; ENDOCRINE DISRUPTING CHEMICALS ; IN-VITRO ESTROGENICITY ; BISPHENOL-A ; DIPHENYL ETHERS ; BLOOD-PLASMA ; BRAIN ; EXPOSURE ; 17-BETA-ESTRADIOL ; IDENTIFICATION |
| 收录类别 | SCI |
| 资助信息 | National Natural Science Foundation of China[20890113]; State Key Laboratory Foundation; Chinese Academy of Sciences[2008FB013]; Natural Science Foundation of Hubei Province, China[2007ABA215] |
| 语种 | 英语 |
| WOS记录号 | WOS:000293834700006 |
| 公开日期 | 2011-12-22 |
| 源URL | [http://ir.ihb.ac.cn/handle/342005/16461]  |
| 专题 | 水生生物研究所_水环境工程研究中心_期刊论文 |
| 作者单位 | Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Jingxian,Shi, Xiongjie,Du, Yongbin,et al. Effects of xenoestrogens on the expression of vitellogenin (vtg) and cytochrome P450 aromatase (cyp19a and b) genes in zebrafish (Danio rerio) larvae[J]. JOURNAL OF ENVIRONMENTAL SCIENCE AND HEALTH PART A-TOXIC/HAZARDOUS SUBSTANCES & ENVIRONMENTAL ENGINEERING,2011,46(9):960-967. |
| APA | Wang, Jingxian,Shi, Xiongjie,Du, Yongbin,&Zhou, Bingsheng.(2011).Effects of xenoestrogens on the expression of vitellogenin (vtg) and cytochrome P450 aromatase (cyp19a and b) genes in zebrafish (Danio rerio) larvae.JOURNAL OF ENVIRONMENTAL SCIENCE AND HEALTH PART A-TOXIC/HAZARDOUS SUBSTANCES & ENVIRONMENTAL ENGINEERING,46(9),960-967. |
| MLA | Wang, Jingxian,et al."Effects of xenoestrogens on the expression of vitellogenin (vtg) and cytochrome P450 aromatase (cyp19a and b) genes in zebrafish (Danio rerio) larvae".JOURNAL OF ENVIRONMENTAL SCIENCE AND HEALTH PART A-TOXIC/HAZARDOUS SUBSTANCES & ENVIRONMENTAL ENGINEERING 46.9(2011):960-967. |
入库方式： OAI收割
来源：水生生物研究所
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