Effects of sexual steroids on the expression of foxl2 in Gobiocypris rarus
文献类型：期刊论文
| 作者 | Jiang, Wenbo1; Yang, Yuhui1; Zhao, Dongmei1; Liu, Xiaoyan1; Duan, Jundan1; Xie, Songguang2; Zhao, Haobin1 |
| 刊名 | COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY
 |
| 出版日期 | 2011-12-01 |
| 卷号 | 160期号:4页码:187-193 |
| 关键词 | Gobiocypris rarus Sex determination and sex differentiation foxl2 foxl2b Expression pattern |
| ISSN号 | 1096-4959 |
| 通讯作者 | Zhao, HB (reprint author), Huazhong Normal Univ, Sch Life Sci, Hubei Key Lab Genet Regulat & Integrated Biol, Wuhan 430079, Hubei, Peoples R China ; zhaohb@mail.ccnu.edu.cn |
| 中文摘要 | Gobiocypris rams is an emerging fish model for aquatic toxicology in China as it is sensitive to environmental hormone disruptors. Exogenous sex steroids can affect sex differentiation and the expression of sex-related genes. Foxl2, a member of forkhead-box transcription factor family, is the key gene for ovary development and its mutation causes the blepharophimosis ptosis epicanthus inversus syndrome in human. We find that two foxl2 genes exist in fish genome, one is foxl2, and the other is foxl2b. Here, we reported the isolation and expression of foxl2 in G. rams. G. rarus foxl2 cDNA is 1700 bp in length with a 921 bp of open reading frame encoding 306 amino acids containing the typical FM-domain. Semi-quantitative RT-PCR revealed its predominant expression in the eye, brain, gill and gonads. Moreover, the expression level in the ovary was significantly higher than that in the testis. Quantitative RT-PCR showed that foxl2 was up regulated after treatment with estradiol and was down regulated with 2-methyl-testosterone. These results suggested that Foxl2 plays an important role in female development of G. rams, foxl2 mRNA expression is regulated by downstream sex hormones, and foxl2 can be used as a molecular indicator monitoring the environmental endocrine disruptors. (C) 2011 Elsevier Inc. All rights reserved. |
| 英文摘要 | Gobiocypris rams is an emerging fish model for aquatic toxicology in China as it is sensitive to environmental hormone disruptors. Exogenous sex steroids can affect sex differentiation and the expression of sex-related genes. Foxl2, a member of forkhead-box transcription factor family, is the key gene for ovary development and its mutation causes the blepharophimosis ptosis epicanthus inversus syndrome in human. We find that two foxl2 genes exist in fish genome, one is foxl2, and the other is foxl2b. Here, we reported the isolation and expression of foxl2 in G. rams. G. rarus foxl2 cDNA is 1700 bp in length with a 921 bp of open reading frame encoding 306 amino acids containing the typical FM-domain. Semi-quantitative RT-PCR revealed its predominant expression in the eye, brain, gill and gonads. Moreover, the expression level in the ovary was significantly higher than that in the testis. Quantitative RT-PCR showed that foxl2 was up regulated after treatment with estradiol and was down regulated with 2-methyl-testosterone. These results suggested that Foxl2 plays an important role in female development of G. rams, foxl2 mRNA expression is regulated by downstream sex hormones, and foxl2 can be used as a molecular indicator monitoring the environmental endocrine disruptors. (C) 2011 Elsevier Inc. All rights reserved. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 学科主题 | Biochemistry & Molecular Biology; Zoology |
| 类目[WOS] | Biochemistry & Molecular Biology ; Zoology |
| 研究领域[WOS] | Biochemistry & Molecular Biology ; Zoology |
| 关键词[WOS] | TRANSCRIPTION FACTOR FOXL2 ; MOLECULAR-CLONING ; GENE-EXPRESSION ; OVARIAN DEVELOPMENT ; OREOCHROMIS-NILOTICUS ; INVERSUS SYNDROME ; ORYZIAS-LATIPES ; NILE TILAPIA ; DIFFERENTIATION ; AROMATASE |
| 收录类别 | SCI |
| 资助信息 | National Key Laboratory of Aquatic Diversity and Resource Conservation; Hubei Key Laboratory of Genetic Regulation and Integrative Biology |
| 语种 | 英语 |
| WOS记录号 | WOS:000296679800007 |
| 公开日期 | 2011-12-22 |
| 源URL | [http://ir.ihb.ac.cn/handle/342005/16411]  |
| 专题 | 水生生物研究所_淡水生态学研究中心_期刊论文 |
| 作者单位 | 1.Huazhong Normal Univ, Sch Life Sci, Hubei Key Lab Genet Regulat & Integrated Biol, Wuhan 430079, Hubei, Peoples R China 2.Chinese Acad Sci, Inst Hydrobiol, Natl Key Lab Aquat Biodivers & Resource Conservat, Wuhan 430072, Hubei, Peoples R China |
| 推荐引用方式 GB/T 7714 | Jiang, Wenbo,Yang, Yuhui,Zhao, Dongmei,et al. Effects of sexual steroids on the expression of foxl2 in Gobiocypris rarus[J]. COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY,2011,160(4):187-193. |
| APA | Jiang, Wenbo.,Yang, Yuhui.,Zhao, Dongmei.,Liu, Xiaoyan.,Duan, Jundan.,...&Zhao, Haobin.(2011).Effects of sexual steroids on the expression of foxl2 in Gobiocypris rarus.COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY,160(4),187-193. |
| MLA | Jiang, Wenbo,et al."Effects of sexual steroids on the expression of foxl2 in Gobiocypris rarus".COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY 160.4(2011):187-193. |
入库方式： OAI收割
来源：水生生物研究所
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