Analysis of MicroRNA Expression in Embryonic Developmental Toxicity Induced by MC-RR
文献类型：期刊论文
| 作者 | Zhao, Yanyan1,2; Xiong, Qian1,2; Xie, Ping1 |
| 刊名 | PLOS ONE
 |
| 出版日期 | 2011-07-29 |
| 卷号 | 6期号:7页码:e22676 |
| 关键词 | CYPRINUS-CARPIO L. MICROCYSTIN-LR GENE-EXPRESSION ZEBRAFISH DEVELOPMENT NEGATIVE REGULATOR RNA-INTERFERENCE DANIO-RERIO CYANOBACTERIA DICER EXTRACT |
| ISSN号 | 1932-6203 |
| 通讯作者 | Zhao, YY (reprint author), Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol China, Donghu Expt Stn Lake Ecosys, Wuhan, Peoples R China ; xieping@ihb.ac.cn |
| 中文摘要 | As cynobacterial blooms frequently occur in fresh waters throughout the world, microcystins (MCs) have caused serious damage to both wildlife and human health. MCs are known to have developmental toxicity, however, the possible molecular mechanism is largely unknown. This is the first toxicological study to integrate post-transcriptomic, proteomic and bioinformatics analysis to explore molecular mechanisms for developmental toxicity of MCs in zebrafish. After being microinjected directly into embryos, MC-RR dose-dependently decreased survival rates and increased malformation rates of embryos, causing various embryo abnormalities including loss of vascular integrity and hemorrhage. Expressions of 31 microRNAs (miRNAs) and 78 proteins were significantly affected at 72 hours post-fertilisation (hpf). Expressions of miR-430 and miR-125 families were also significantly changed. The altered expressions of miR-31 and miR-126 were likely responsible for the loss of vascular integrity. MC-RR significantly reduced the expressions of a number of proteins involved in energy metabolism, cell division, protein synthesis, cytoskeleton maintenance, response to stress and DNA replication. Bioinformatics analysis shows that several aberrantly expressed miRNAs and proteins (involved in various molecular pathways) were predicted to be potential MC-responsive miRNA-target pairs, and that their aberrant expressions should be the possible molecular mechanisms for the various developmental defects caused by MC-RR. |
| 英文摘要 | As cynobacterial blooms frequently occur in fresh waters throughout the world, microcystins (MCs) have caused serious damage to both wildlife and human health. MCs are known to have developmental toxicity, however, the possible molecular mechanism is largely unknown. This is the first toxicological study to integrate post-transcriptomic, proteomic and bioinformatics analysis to explore molecular mechanisms for developmental toxicity of MCs in zebrafish. After being microinjected directly into embryos, MC-RR dose-dependently decreased survival rates and increased malformation rates of embryos, causing various embryo abnormalities including loss of vascular integrity and hemorrhage. Expressions of 31 microRNAs (miRNAs) and 78 proteins were significantly affected at 72 hours post-fertilisation (hpf). Expressions of miR-430 and miR-125 families were also significantly changed. The altered expressions of miR-31 and miR-126 were likely responsible for the loss of vascular integrity. MC-RR significantly reduced the expressions of a number of proteins involved in energy metabolism, cell division, protein synthesis, cytoskeleton maintenance, response to stress and DNA replication. Bioinformatics analysis shows that several aberrantly expressed miRNAs and proteins (involved in various molecular pathways) were predicted to be potential MC-responsive miRNA-target pairs, and that their aberrant expressions should be the possible molecular mechanisms for the various developmental defects caused by MC-RR. |
| WOS标题词 | Science & Technology |
| 学科主题 | Life Sciences & Biomedicine - Other Topics |
| 类目[WOS] | Multidisciplinary Sciences |
| 研究领域[WOS] | Science & Technology - Other Topics |
| 关键词[WOS] | CYPRINUS-CARPIO L. ; MICROCYSTIN-LR ; GENE-EXPRESSION ; ZEBRAFISH DEVELOPMENT ; NEGATIVE REGULATOR ; RNA-INTERFERENCE ; DANIO-RERIO ; CYANOBACTERIA ; DICER ; EXTRACT |
| 收录类别 | SCI |
| 资助信息 | National Basic Research Program of China (973 Program)[2008CB418101]; National Natural Science Foundation of China[31070457] |
| 语种 | 英语 |
| WOS记录号 | WOS:000293286500017 |
| 公开日期 | 2011-12-22 |
| 源URL | [http://ir.ihb.ac.cn/handle/342005/16431]  |
| 专题 | 水生生物研究所_淡水生态学研究中心_期刊论文 |
| 作者单位 | 1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol China, Donghu Expt Stn Lake Ecosys, Wuhan, Peoples R China 2.Chinese Acad Sci, Grad Sch, Wuhan, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhao, Yanyan,Xiong, Qian,Xie, Ping. Analysis of MicroRNA Expression in Embryonic Developmental Toxicity Induced by MC-RR[J]. PLOS ONE,2011,6(7):e22676. |
| APA | Zhao, Yanyan,Xiong, Qian,&Xie, Ping.(2011).Analysis of MicroRNA Expression in Embryonic Developmental Toxicity Induced by MC-RR.PLOS ONE,6(7),e22676. |
| MLA | Zhao, Yanyan,et al."Analysis of MicroRNA Expression in Embryonic Developmental Toxicity Induced by MC-RR".PLOS ONE 6.7(2011):e22676. |
入库方式： OAI收割
来源：水生生物研究所
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