Acquisition of Humoral Transplantation Tolerance upon De Novo Emergence of B Lymphocytes
文献类型：期刊论文
| 作者 | Parsons, Ronald F.1; Vivek, Kumar1; Rostami, Susan Y.1; Zekavat, Ghazal1; Ziaie, Seyed M.1; Luo, Yanping1,3; Koeberlein, Brigitte1; Redfield, Robert R.1; Cancro, Michael P.2; Naji, Ali1 |
| 刊名 | JOURNAL OF IMMUNOLOGY
 |
| 出版日期 | 2011 |
| 卷号 | 186期号:1页码:614-620 |
| ISSN号 | 0022-1767 |
| 关键词 | DONOR-SPECIFIC TRANSFUSIONS HEAT-STABLE ANTIGEN(HI) MAINTENANCE IMMUNOSUPPRESSION ALLOGRAFT RECIPIENTS PERIPHERAL DELETION CHRONIC REJECTION CELL MATURATION MIXED CHIMERISM CLONAL DELETION T-CELL |
| 通讯作者 | Noorchashm, H (reprint author), Univ Penn, Sch Med, Harrison Dept Surg Res, 329 Stemmler Hall, Philadelphia, PA 19104 USA ; hooman.noorchashm@uphs.upenn.edu |
| 中文摘要 | A major obstacle to transplantation tolerance is humoral immunity. In this paper, we demonstrate that the intrinsic developmental propensity of the B lymphocyte compartment for acquisition of self-tolerance can be harnessed to induce humoral unresponsiveness to transplanted alloantigens. In the current study, when transitional B cells developed in the presence of donor lymphoid cells, the mature B lymphocyte compartment failed to mount a donor-specific alloantibody response to an organ transplant-despite unrestrained acute T cell-mediated allograft rejection. Specifically, we generated an experimental system wherein a B6 strain B cell compartment developed de novo in the presence of F1 (B6xBALB/c) lymphoid cells and in a T cell-deficient setting. Following establishment of a steady-state B cell compartment, these B6 mice were transplanted with heterotopic cardiac allografts from allogeneic BALB/c donors. The mice were then inoculated with purified syngeneic B6 T cells. As expected, all cardiac allografts were acutely rejected. However, the B lymphocyte compartment of these mice was completely inert in its capacity to form a BALB/c-specific alloantibody response. Using an alloantigen-specific Ig transgenic system, we demonstrated that this profound degree of humoral tolerance was caused by clonal deletion of alloreactive specificities from the primary B cell repertoire. Thus, de novo B cell compartment development at the time of transplantation is of critical importance in recipient repertoire "remodeling" to a humoral tolerant state. The Journal of Immunology, 2011, 186: 614-620. |
| 英文摘要 | A major obstacle to transplantation tolerance is humoral immunity. In this paper, we demonstrate that the intrinsic developmental propensity of the B lymphocyte compartment for acquisition of self-tolerance can be harnessed to induce humoral unresponsiveness to transplanted alloantigens. In the current study, when transitional B cells developed in the presence of donor lymphoid cells, the mature B lymphocyte compartment failed to mount a donor-specific alloantibody response to an organ transplant-despite unrestrained acute T cell-mediated allograft rejection. Specifically, we generated an experimental system wherein a B6 strain B cell compartment developed de novo in the presence of F1 (B6xBALB/c) lymphoid cells and in a T cell-deficient setting. Following establishment of a steady-state B cell compartment, these B6 mice were transplanted with heterotopic cardiac allografts from allogeneic BALB/c donors. The mice were then inoculated with purified syngeneic B6 T cells. As expected, all cardiac allografts were acutely rejected. However, the B lymphocyte compartment of these mice was completely inert in its capacity to form a BALB/c-specific alloantibody response. Using an alloantigen-specific Ig transgenic system, we demonstrated that this profound degree of humoral tolerance was caused by clonal deletion of alloreactive specificities from the primary B cell repertoire. Thus, de novo B cell compartment development at the time of transplantation is of critical importance in recipient repertoire "remodeling" to a humoral tolerant state. The Journal of Immunology, 2011, 186: 614-620. |
| 学科主题 | Immunology |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Immunology |
| 研究领域[WOS] | Immunology |
| 关键词[WOS] | DONOR-SPECIFIC TRANSFUSIONS ; HEAT-STABLE ANTIGEN(HI) ; MAINTENANCE IMMUNOSUPPRESSION ; ALLOGRAFT RECIPIENTS ; PERIPHERAL DELETION ; CHRONIC REJECTION ; CELL MATURATION ; MIXED CHIMERISM ; CLONAL DELETION ; T-CELL |
| 资助信息 | National Institutes of Health[KO8-DK064603, RO3-DK080286, T32DK007314-29]; Juvenile Diabetes Research Foundation[4-2008-351] |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000285688700069 |
| 公开日期 | 2011-12-22 |
| 源URL | [http://ir.ihb.ac.cn/handle/342005/16443]  |
| 专题 | 水生生物研究所_鱼类生物学及渔业生物技术研究中心_期刊论文 |
| 作者单位 | 1.Univ Penn, Sch Med, Harrison Dept Surg Res, Philadelphia, PA 19104 USA 2.Univ Penn, Sch Med, Dept Pathol & Lab Med, Philadelphia, PA 19104 USA 3.Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan, Peoples R China |
| 推荐引用方式 GB/T 7714 | Parsons, Ronald F.,Vivek, Kumar,Rostami, Susan Y.,et al. Acquisition of Humoral Transplantation Tolerance upon De Novo Emergence of B Lymphocytes[J]. JOURNAL OF IMMUNOLOGY,2011,186(1):614-620. |
| APA | Parsons, Ronald F..,Vivek, Kumar.,Rostami, Susan Y..,Zekavat, Ghazal.,Ziaie, Seyed M..,...&Noorchashm, Hooman.(2011).Acquisition of Humoral Transplantation Tolerance upon De Novo Emergence of B Lymphocytes.JOURNAL OF IMMUNOLOGY,186(1),614-620. |
| MLA | Parsons, Ronald F.,et al."Acquisition of Humoral Transplantation Tolerance upon De Novo Emergence of B Lymphocytes".JOURNAL OF IMMUNOLOGY 186.1(2011):614-620. |
入库方式： OAI收割
来源：水生生物研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。