Karyotype analysis of Oreochromis mossambicus, O. urolepis hornorum and their hybrid based on Cot-1 DNA bands by fluorescence in situ hybridization
文献类型：期刊论文
| 作者 | Zhu, Hua Ping1; Lu, Mai Xin1; Huang, Zhang Han1; Gao, Feng Ying1; Ma, Dong Mei1; Zhou, Li2; Gui, Jian Fang2 |
| 刊名 | AQUACULTURE RESEARCH
 |
| 出版日期 | 2011-07-01 |
| 卷号 | 42期号:8页码:1178-1185 |
| 关键词 | tilapia karyotype Cot-1 DNA fluorescence in situ hybridization (FISH) |
| ISSN号 | 1355-557X |
| 通讯作者 | Lu, MX (reprint author), Chinese Acad Fishery Sci, Pearl River Fisheries Res Inst, Guangzhou 510380, Guangdong, Peoples R China ; mx-lu@163.com |
| 中文摘要 | Chromosomal karyotypes of Oreochromis mossambicus and O. urolepis hornorum and their hybrid were analysed by means of Cot-1 DNA bandings through fluorescence in situ hybridization (FISH). To identify all chromosomes, Cot-1DNA - which contains highly and moderately repetitive DNA - was extracted from genomic DNA, labelled as a probe with Dig-11-dUTP, and in situ hybridized to spreads of mitotic chromosomes of the three samples. The hybridized signals were detected by means of Cy3-conjugated antidigoxigenin. The FISH results indicated that the three samples had the same diploid number (2n = 44) of chromosomes. Specific fluorescence signal bands were detected on all individual chromosome pairs. On the basis of Cot-1 DNA FISH banding patterns and chromosome morphology, the karyotypes of the three samples have been constructed; no remarkable differences were detected between the karyotypes of these species using this method. These results - which are similar to those reported previously, with respect to chromosome number, morphology and Cot-1DNA FISH patterns - suggest chromosomal stasis during speciation and hybridization of tilapia (Oreochromis, Cichlidae). Such a molecular cytogenetic procedure, if used in conjunction with other genomic research methods, could facilitate the study of genomic structure and be adapted for chromosome studies of other animal species. |
| 英文摘要 | Chromosomal karyotypes of Oreochromis mossambicus and O. urolepis hornorum and their hybrid were analysed by means of Cot-1 DNA bandings through fluorescence in situ hybridization (FISH). To identify all chromosomes, Cot-1DNA - which contains highly and moderately repetitive DNA - was extracted from genomic DNA, labelled as a probe with Dig-11-dUTP, and in situ hybridized to spreads of mitotic chromosomes of the three samples. The hybridized signals were detected by means of Cy3-conjugated antidigoxigenin. The FISH results indicated that the three samples had the same diploid number (2n = 44) of chromosomes. Specific fluorescence signal bands were detected on all individual chromosome pairs. On the basis of Cot-1 DNA FISH banding patterns and chromosome morphology, the karyotypes of the three samples have been constructed; no remarkable differences were detected between the karyotypes of these species using this method. These results - which are similar to those reported previously, with respect to chromosome number, morphology and Cot-1DNA FISH patterns - suggest chromosomal stasis during speciation and hybridization of tilapia (Oreochromis, Cichlidae). Such a molecular cytogenetic procedure, if used in conjunction with other genomic research methods, could facilitate the study of genomic structure and be adapted for chromosome studies of other animal species. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 学科主题 | Fisheries |
| 类目[WOS] | Fisheries |
| 研究领域[WOS] | Fisheries |
| 关键词[WOS] | NILE TILAPIA ; REPETITIVE SEQUENCES ; SEX-CHROMOSOMES ; C(0)T-1 DNA ; NILOTICUS ; EVOLUTION ; CICHLIDAE ; ELEMENTS ; GENOME ; FISH |
| 收录类别 | SCI |
| 资助信息 | Special Scientific Research Fund for Commonwealth Agricultural Industry of China[3-49]; Key Program for Agriculture Research of Guangdong Province[2009B020201003]; Natural Science Foundation of Guangdong Province[9451038001003595]; Earmarked Fund for Modern Agro-industry Technology Research System[nycytx-48-4]; Marine Fisheries Science and Technology Promotion Project of Guangdong Province[A200901C01]; Open Foundation of the State Key Laboratory of Freshwater Ecology and Biotechnology of China[2009FB03] |
| 语种 | 英语 |
| WOS记录号 | WOS:000292650100014 |
| 公开日期 | 2011-12-22 |
| 源URL | [http://ir.ihb.ac.cn/handle/342005/16445]  |
| 专题 | 水生生物研究所_鱼类生物学及渔业生物技术研究中心_期刊论文 |
| 作者单位 | 1.Chinese Acad Fishery Sci, Pearl River Fisheries Res Inst, Guangzhou 510380, Guangdong, Peoples R China 2.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan, Hubei, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhu, Hua Ping,Lu, Mai Xin,Huang, Zhang Han,et al. Karyotype analysis of Oreochromis mossambicus, O. urolepis hornorum and their hybrid based on Cot-1 DNA bands by fluorescence in situ hybridization[J]. AQUACULTURE RESEARCH,2011,42(8):1178-1185. |
| APA | Zhu, Hua Ping.,Lu, Mai Xin.,Huang, Zhang Han.,Gao, Feng Ying.,Ma, Dong Mei.,...&Gui, Jian Fang.(2011).Karyotype analysis of Oreochromis mossambicus, O. urolepis hornorum and their hybrid based on Cot-1 DNA bands by fluorescence in situ hybridization.AQUACULTURE RESEARCH,42(8),1178-1185. |
| MLA | Zhu, Hua Ping,et al."Karyotype analysis of Oreochromis mossambicus, O. urolepis hornorum and their hybrid based on Cot-1 DNA bands by fluorescence in situ hybridization".AQUACULTURE RESEARCH 42.8(2011):1178-1185. |
入库方式： OAI收割
来源：水生生物研究所
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