Tumor-Specific Expression Detected with the Use of an Expression Vector Driven by Human Telomerase Reverse Transcriptase Gene Promoter
文献类型:期刊论文
作者 | Yin JJ; Fu PF; Wang XB![]() ![]() |
刊名 | Journal of Laboratory and Clinical Medicine
![]() |
出版日期 | 2004 |
卷号 | 144期号:6页码:302-306 |
通讯作者邮箱 | xiurj@yahoo.com.cn |
ISSN号 | 0022-2143 |
通讯作者 | Xiu, RJ (reprint author), Peking Union Med Coll, Chinese Acad Med Sci, Inst Microcirculat, Beijing 100005, Peoples R China. |
中文摘要 | To construct the tumor-specific expression vector driven by human telomerase reserve transcriptase gene promoter, we amplified a fragment of the enhanced green fluorescent protein (EGFP) gene from the pEGFP-N 1 plasmid and cloned it into the multiple cloning site of the pLNCX vector, then named the recombinant as pLNCX-EGFP. The fragment of human telomerase reverse transcriptase gene promoter (hTERT) was amplified from the human genome with the use of human telomerase reserve transcriptase gene-specific primers and cloned into the pLNCX-EGFP vector, from which the cytomegalovirus promoter had previously been removed through the use of restriction enzymes, in sense orientation relative to the green fluorescent protein coding sequence. Then the expression vector pLNT-EGFP-under the control of the human telomerase reserve transcriptase gene promoter, which contains green fluorescent protein reporter gene-was successfully constructed. To detect the transcriptional activity of the human telomerase reserve transcriptase gene promoter, we conducted transient transfection of this specific expression vector into human lung fibroblast (HLF) cell lines with high telomerase activity and normal human fetal lung fibroblast (W138) cell lines without telomerase activity. The results of transient transfection showed that the pLNT-EGFP vector strongly expressed the green fluorescent protein reporter gene in telomerase-positive cells but not in telomerase-negative cells. |
学科主题 | 力学 |
类目[WOS] | Medical Laboratory Technology ; Medicine, General & Internal ; Medicine, Research & Experimental |
研究领域[WOS] | Medical Laboratory Technology ; General & Internal Medicine ; Research & Experimental Medicine |
关键词[WOS] | CANCER-CELLS ; CLONING ; HTERT |
收录类别 | SCI |
语种 | 英语 |
WOS记录号 | WOS:000225891100004 |
公开日期 | 2007-06-15 ; 2007-12-05 ; 2009-06-23 |
源URL | [http://dspace.imech.ac.cn/handle/311007/15334] ![]() |
专题 | 力学研究所_力学所知识产出(1956-2008) |
推荐引用方式 GB/T 7714 | Yin JJ,Fu PF,Wang XB,et al. Tumor-Specific Expression Detected with the Use of an Expression Vector Driven by Human Telomerase Reverse Transcriptase Gene Promoter[J]. Journal of Laboratory and Clinical Medicine,2004,144(6):302-306. |
APA | Yin JJ,Fu PF,Wang XB,靳刚,&Xiu RJ.(2004).Tumor-Specific Expression Detected with the Use of an Expression Vector Driven by Human Telomerase Reverse Transcriptase Gene Promoter.Journal of Laboratory and Clinical Medicine,144(6),302-306. |
MLA | Yin JJ,et al."Tumor-Specific Expression Detected with the Use of an Expression Vector Driven by Human Telomerase Reverse Transcriptase Gene Promoter".Journal of Laboratory and Clinical Medicine 144.6(2004):302-306. |
入库方式: OAI收割
来源:力学研究所
浏览0
下载0
收藏0
其他版本
除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。