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Tumor-Specific Expression Detected with the Use of an Expression Vector Driven by Human Telomerase Reverse Transcriptase Gene Promoter

文献类型：期刊论文

作者Yin JJ; Fu PF; Wang XB; Jin G(靳刚); Xiu RJ
刊名Journal of Laboratory and Clinical Medicine
出版日期2004
卷号144期号:6页码:302-306
通讯作者邮箱xiurj@yahoo.com.cn
ISSN号0022-2143
通讯作者Xiu, RJ (reprint author), Peking Union Med Coll, Chinese Acad Med Sci, Inst Microcirculat, Beijing 100005, Peoples R China.
中文摘要To construct the tumor-specific expression vector driven by human telomerase reserve transcriptase gene promoter, we amplified a fragment of the enhanced green fluorescent protein (EGFP) gene from the pEGFP-N 1 plasmid and cloned it into the multiple cloning site of the pLNCX vector, then named the recombinant as pLNCX-EGFP. The fragment of human telomerase reverse transcriptase gene promoter (hTERT) was amplified from the human genome with the use of human telomerase reserve transcriptase gene-specific primers and cloned into the pLNCX-EGFP vector, from which the cytomegalovirus promoter had previously been removed through the use of restriction enzymes, in sense orientation relative to the green fluorescent protein coding sequence. Then the expression vector pLNT-EGFP-under the control of the human telomerase reserve transcriptase gene promoter, which contains green fluorescent protein reporter gene-was successfully constructed. To detect the transcriptional activity of the human telomerase reserve transcriptase gene promoter, we conducted transient transfection of this specific expression vector into human lung fibroblast (HLF) cell lines with high telomerase activity and normal human fetal lung fibroblast (W138) cell lines without telomerase activity. The results of transient transfection showed that the pLNT-EGFP vector strongly expressed the green fluorescent protein reporter gene in telomerase-positive cells but not in telomerase-negative cells.
学科主题力学
类目[WOS]Medical Laboratory Technology ; Medicine, General & Internal ; Medicine, Research & Experimental
研究领域[WOS]Medical Laboratory Technology ; General & Internal Medicine ; Research & Experimental Medicine
关键词[WOS]CANCER-CELLS ; CLONING ; HTERT
收录类别SCI
语种英语
WOS记录号WOS:000225891100004
公开日期2007-06-15 ; 2007-12-05 ; 2009-06-23
源URL[http://dspace.imech.ac.cn/handle/311007/15334]  
专题力学研究所_力学所知识产出(1956-2008)
推荐引用方式
GB/T 7714		 Yin JJ,Fu PF,Wang XB,et al. Tumor-Specific Expression Detected with the Use of an Expression Vector Driven by Human Telomerase Reverse Transcriptase Gene Promoter[J]. Journal of Laboratory and Clinical Medicine,2004,144(6):302-306.
APA Yin JJ,Fu PF,Wang XB,靳刚,&Xiu RJ.(2004).Tumor-Specific Expression Detected with the Use of an Expression Vector Driven by Human Telomerase Reverse Transcriptase Gene Promoter.Journal of Laboratory and Clinical Medicine,144(6),302-306.
MLA Yin JJ,et al."Tumor-Specific Expression Detected with the Use of an Expression Vector Driven by Human Telomerase Reverse Transcriptase Gene Promoter".Journal of Laboratory and Clinical Medicine 144.6(2004):302-306.

入库方式： OAI收割

来源：力学研究所

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