A Microplate Fluorimetric Assay for Sacchariase Activity Measurement
文献类型：期刊论文
| 作者 | Zhang, LL ; Wu, ZJ ; Chen, LJ ; Li, DP ; Ma, XZ ; Shi, YF |
| 刊名 | SPECTROSCOPY AND SPECTRAL ANALYSIS
 |
| 出版日期 | 2009 |
| 卷号 | 29期号:5页码:1341-1344 |
| 关键词 | Fluorescence detection Microplate FACE(free-air carbon dioxide enrichment) Sacchariase activity |
| ISSN号 | 1000-0593 |
| 通讯作者 | Wu, ZJ, Chinese Acad Sci, Inst Appl Ecol, Shenyang 110016, Peoples R China |
| 中文摘要 | With the fluorescent compound conjugates substrates, soil xylanase and cellulose in a free-air carbon dioxide enrichment (FACE) experiment were measured on the base of 96 microplate and fluorescence detection, aiming at testing its feasibility in sacchariase activity measurement. The results show that sacchariase activity can be tested and the data exhibit better repeatability(coefficient of variability <= 4.879%). Compared with spectrophotometric assay, this method allows a large number of soil samples and/or enzymes to be analyzed in a short time accurately and conveniently. Soil xylanase activity tends to be greater at elevated CO2 which significantly increases in jointing, heading and ripening stages of wheat and in heading and ripening stages of rice (P<0.05), and the crop metabolizes rapidly under FACE condition and soil microorganisms are affected, which causes elevation of xylanase acitivy. Compared with ambient CO2, soil cellulose activity decreased slightly under elevated CO2 but there was no significant difference between treatments, indicating the cellulose activity was not influenced intensively in a short time. |
| 学科主题 | Spectroscopy |
| 收录类别 | SCI |
| 语种 | 中文 |
| WOS记录号 | WOS:000265756100044 |
| 公开日期 | 2011-09-23 |
| 源URL | [http://210.72.129.5/handle/321005/54161]  |
| 专题 | 沈阳应用生态研究所_沈阳应用生态研究所_期刊论文 |
| 推荐引用方式 GB/T 7714 | Zhang, LL,Wu, ZJ,Chen, LJ,et al. A Microplate Fluorimetric Assay for Sacchariase Activity Measurement[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS,2009,29(5):1341-1344. |
| APA | Zhang, LL,Wu, ZJ,Chen, LJ,Li, DP,Ma, XZ,&Shi, YF.(2009).A Microplate Fluorimetric Assay for Sacchariase Activity Measurement.SPECTROSCOPY AND SPECTRAL ANALYSIS,29(5),1341-1344. |
| MLA | Zhang, LL,et al."A Microplate Fluorimetric Assay for Sacchariase Activity Measurement".SPECTROSCOPY AND SPECTRAL ANALYSIS 29.5(2009):1341-1344. |
入库方式： OAI收割
来源：沈阳应用生态研究所
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