Enhancement of superantigen activity and antitumor response of staphylococcal enterotoxin C2 by site-directed mutagenesis
文献类型：期刊论文
| 作者 | Wang, XG ; Xu, MK ; Zhang, HW ; Liu, J ; Li, X ; Zhang, CG |
| 刊名 | CANCER IMMUNOLOGY IMMUNOTHERAPY
 |
| 出版日期 | 2009 |
| 卷号 | 58期号:5页码:677-686 |
| 关键词 | Superantigen Staphylococcal enterotoxin Antitumor Immunosuppression Endotoxic activity Site-directed mutagenesis |
| ISSN号 | 0340-7004 |
| 通讯作者 | Zhang, HW, Chinese Acad Sci, Inst Appl Ecol, Shenyang 110016, Peoples R China |
| 中文摘要 | Bacterial superantigen staphylococcal enterotoxins (SEs) tremendously stimulate polyclonal T cells bearing particular TCR V beta domains when binding to MHC II molecules, suggesting that they could be a candidate of new antitumor agent. SEC2, an important member of superantigen family, has been used in clinical trial as an immuntherapy agent for cancer treatment in China, and obtained some encouraging effects. However, the presence of immunosuppression and endotoxic activity limits the therapeutic dosage of SEC2, and influences its antitumor effect in clinic. Therefore, the enhancement of superantigen activity and antitumor effect of SEC2 could effectively make compensation for the disadvantages mentioned above. In this study, a superantigen SEC2(T20L/G22E) mutant was generated by site-directed mutagenesis, and efficiently expressed in E. coli BL21(DE3). The results showed that SEC2(T20L/G22E) mutant exhibited a significantly enhanced superantigen activity and antitumor response, compared with native SEC2 in vitro. Further toxicity assay in vivo indicated that SEC2(T20L/G22E) mutant had no significant increase in emetic and pyrogenic activity compared with SEC2, which suggested that the mutant SEC2(T20L/G22E) could be used as a potentially powerful candidate for cancer immunotherapy, and could make compensation for the deficiency of native SEC2 in clinic. |
| 学科主题 | Oncology; Immunology |
| 收录类别 | SCI |
| 资助信息 | Institute of Applied Ecology of the CAS [06LYQYC001, 08SBS111S3] |
| 语种 | 英语 |
| WOS记录号 | WOS:000263671400004 |
| 公开日期 | 2011-09-23 |
| 源URL | [http://210.72.129.5/handle/321005/54221]  |
| 专题 | 沈阳应用生态研究所_沈阳应用生态研究所_期刊论文 |
| 推荐引用方式 GB/T 7714 | Wang, XG,Xu, MK,Zhang, HW,et al. Enhancement of superantigen activity and antitumor response of staphylococcal enterotoxin C2 by site-directed mutagenesis[J]. CANCER IMMUNOLOGY IMMUNOTHERAPY,2009,58(5):677-686. |
| APA | Wang, XG,Xu, MK,Zhang, HW,Liu, J,Li, X,&Zhang, CG.(2009).Enhancement of superantigen activity and antitumor response of staphylococcal enterotoxin C2 by site-directed mutagenesis.CANCER IMMUNOLOGY IMMUNOTHERAPY,58(5),677-686. |
| MLA | Wang, XG,et al."Enhancement of superantigen activity and antitumor response of staphylococcal enterotoxin C2 by site-directed mutagenesis".CANCER IMMUNOLOGY IMMUNOTHERAPY 58.5(2009):677-686. |
入库方式： OAI收割
来源：沈阳应用生态研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。