Bioinformatic and Expression Analyses of Genes Mediating Zinc Homeostasis in Nostoc punctiforme
文献类型：期刊论文
| 作者 | Hudek, L ; Rai, LC ; Freestone, D ; Michalczyk, A ; Gibson, M ; Song, YF ; Ackland, ML |
| 刊名 | APPLIED AND ENVIRONMENTAL MICROBIOLOGY
 |
| 出版日期 | 2009 |
| 卷号 | 75期号:3页码:784-791 |
| ISSN号 | 0099-2240 |
| 通讯作者 | Ackland, ML, Deakin Univ, Ctr Cellular & Mol Biol, Sch Life & Environm Sci, Burwood Campus,221 Burwood Highway, Burwood, Vic 3125, Australia |
| 中文摘要 | Zinc homeostasis was investigated in Nostoc punctiforme. Cell tolerance to Zn2+ over 14 days showed that ZnCl2 levels above 22 mu M significantly reduced cell viability. After 3 days in 22 mu M ZnCl2, ca. 12% of the Zn2+ was in an EDTA-resistant component, suggesting an intracellular localization. Zinquin fluorescence was detected within cells exposed to concentrations up to 37 mu M relative to 0 mu M treatment. Radiolabeled Zn-65 showed Zn2+ uptake increased over a 3-day period, while efflux occurred more rapidly within a 3-h time period. Four putative genes involved in Zn2+ uptake and efflux in N. punctiforme were identified: (i) the predicted Co/Zn/Cd cation transporter, putative CDF; (ii) the predicted divalent heavy-metal cation transporter, putative Zip; (iii) the ATPase component and Fe/Zn uptake regulation protein, putative Fur; and (iv) an ABC-type Mn/Zn transport system, putative zinc ZnuC, ZnuABC system component. Quantitative real-time PCR indicated the responsiveness of all four genes to 22 mu M ZnCl2 within 3 h, followed by a reduction to below basal levels after 24 h by putative ZIP, ZnuC, and Fur and a reduction to below basal level after 72 h by putative CDF efflux gene. These results demonstrate differential regulation of zinc transporters over time, indicating a role for them in zinc homeostasis in N. punctiforme. |
| 学科主题 | Biotechnology & Applied Microbiology; Microbiology |
| 收录类别 | SCI |
| 资助信息 | International Science Linkage Programme ; Australia-China Special Fund for Scientific and Technological Cooperation. |
| 语种 | 英语 |
| WOS记录号 | WOS:000262690100029 |
| 公开日期 | 2011-09-23 |
| 源URL | [http://210.72.129.5/handle/321005/54261]  |
| 专题 | 沈阳应用生态研究所_沈阳应用生态研究所_期刊论文 |
| 推荐引用方式 GB/T 7714 | Hudek, L,Rai, LC,Freestone, D,et al. Bioinformatic and Expression Analyses of Genes Mediating Zinc Homeostasis in Nostoc punctiforme[J]. APPLIED AND ENVIRONMENTAL MICROBIOLOGY,2009,75(3):784-791. |
| APA | Hudek, L.,Rai, LC.,Freestone, D.,Michalczyk, A.,Gibson, M.,...&Ackland, ML.(2009).Bioinformatic and Expression Analyses of Genes Mediating Zinc Homeostasis in Nostoc punctiforme.APPLIED AND ENVIRONMENTAL MICROBIOLOGY,75(3),784-791. |
| MLA | Hudek, L,et al."Bioinformatic and Expression Analyses of Genes Mediating Zinc Homeostasis in Nostoc punctiforme".APPLIED AND ENVIRONMENTAL MICROBIOLOGY 75.3(2009):784-791. |
入库方式： OAI收割
来源：沈阳应用生态研究所
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