Cadmium stress alters gene expression of DNA mismatch repair related genes in Arabidopsis seedlings
文献类型：期刊论文
| 作者 | Liu, W ; Yang, YS ; Francis, D ; Rogers, HJ ; Li, P ; Zhang, Q |
| 刊名 | CHEMOSPHERE
 |
| 出版日期 | 2008 |
| 卷号 | 73期号:7页码:1138-1144 |
| 关键词 | Cadmium Gene expression DNA mismatch repair genes PCNA genes Biomarker |
| ISSN号 | 0045-6535 |
| 通讯作者 | Yang, YS, Cardiff Univ, Sch Earth Sci, Cardiff CF10 3YE, S Glam, Wales |
| 中文摘要 | Cadmium (Cd) is a non essential element, and is a widespread environmental pollutant. Exposure to Cd can result in a variety of adverse health effects in plant and humans. In the current study, Arabidopsis seedlings were used as a bio-indicator of Cd pollution. Seedlings were grown on MS media containing 0-6.0 mg L-1 Cd for 18 days, and the gene expression patterns were used to link increased Cd exposure with progressive biological effects. Reduction of total soluble protein content in shoots of the Arabidopsis seedlings occurred with increase in Cd concentrations. For the gene expression patterns, seven genes known to be involved in cell division and DNA mismatch repair (MMR) system were investigated by semi-quantitative RT-PCR, and normalized using 18 S rRNA gene expression. Expression of the proliferating cell nuclear antigen 2 (atPCNA 2), MutS 3 homolog (atMSH 3) and MutL1 homolog (atMLH1) genes in shoots of Arabidopsis was strongly induced by exposure to 0.75 mg L-1 Cd, but were repressed by other Cd concentrations whereas exposure to 0.75-6 mg L-1 of Cd resulted in a decreased expression of atPCNA1, atMSH 2, 6 and 7 genes independently of any observable biological effects, including survival, fresh weight and chlorophyll level of shoots. This work demonstrated that specific gene expression changes could serve as useful molecular biomarkers indicative of Cd exposure and related biological effects. (C) 2008 Elsevier Ltd. All rights reserved. |
| 学科主题 | Environmental Sciences |
| 收录类别 | SCI |
| 资助信息 | Royal Society BP ; Knowledge Innovation Key Program [kzcx1-yw-06-03]; NSFC [20377043]; 973 Project [2004CB418506]; China MOE |
| 语种 | 英语 |
| WOS记录号 | WOS:000260861600018 |
| 公开日期 | 2011-09-23 |
| 源URL | [http://210.72.129.5/handle/321005/54319]  |
| 专题 | 沈阳应用生态研究所_沈阳应用生态研究所_期刊论文 |
| 推荐引用方式 GB/T 7714 | Liu, W,Yang, YS,Francis, D,et al. Cadmium stress alters gene expression of DNA mismatch repair related genes in Arabidopsis seedlings[J]. CHEMOSPHERE,2008,73(7):1138-1144. |
| APA | Liu, W,Yang, YS,Francis, D,Rogers, HJ,Li, P,&Zhang, Q.(2008).Cadmium stress alters gene expression of DNA mismatch repair related genes in Arabidopsis seedlings.CHEMOSPHERE,73(7),1138-1144. |
| MLA | Liu, W,et al."Cadmium stress alters gene expression of DNA mismatch repair related genes in Arabidopsis seedlings".CHEMOSPHERE 73.7(2008):1138-1144. |
入库方式： OAI收割
来源：沈阳应用生态研究所
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