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PCR-DGGE analysis of nematode diversity in Cu-contaminated soil

文献类型：期刊论文

作者Wang, SB ; Li, Q ; Liang, WJ ; Jiang, Y ; Jiang, SW
刊名PEDOSPHERE
出版日期2008
卷号18期号:5页码:621-627
关键词copper contamination morphological analysis nematode diversity PCR-DGGE
ISSN号1002-0160
通讯作者Liang, WJ, Chinese Acad Sci, Inst Appl Ecol, Key Lab Terr Ecol Proc, Shenyang 110016, Peoples R China
中文摘要A wheat pot experiment was conducted tinder greenhouse conditions to assess the effect of copper contamination oil soil nematode diversity by polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE) method and morphological analysis. The soil was treated with CuSO4 center dot 5H(2)O at the following concentrations: 0, 50; 100 200 400, and 800 mg kg(-1) dry soil, and the soil samples were collected at wheat jointing and ripening stages. Nematode diversity index (H ') from morphological analysis showed no difference between the control and the treated samples in either of the sampling dates. At the wheat ripening stage, nematode diversity obtained by the PCR-DGGE method decreased noticeably in the Cu800 treatment in comparison with the control. With optimization of the method of nematode DNA extraction, PCR-DGGE could give more information oil nematode genera, and the intensity of the bands could reflect the abundance of nematode genera in the assemblage. The PCR-DGGE method proved promising in distinguishing nematode diversity in heavy metal contaminated soil.
学科主题Soil Science
收录类别SCI
资助信息National Natural Science Foundation of China [30600087]; Chinese Academy of Sciences [06LYQY5001]
语种英语
WOS记录号WOS:000259219500009
公开日期2011-09-23
源URL[http://210.72.129.5/handle/321005/54385]  
专题沈阳应用生态研究所_沈阳应用生态研究所_期刊论文
推荐引用方式
GB/T 7714		 Wang, SB,Li, Q,Liang, WJ,et al. PCR-DGGE analysis of nematode diversity in Cu-contaminated soil[J]. PEDOSPHERE,2008,18(5):621-627.
APA Wang, SB,Li, Q,Liang, WJ,Jiang, Y,&Jiang, SW.(2008).PCR-DGGE analysis of nematode diversity in Cu-contaminated soil.PEDOSPHERE,18(5),621-627.
MLA Wang, SB,et al."PCR-DGGE analysis of nematode diversity in Cu-contaminated soil".PEDOSPHERE 18.5(2008):621-627.

入库方式： OAI收割

来源：沈阳应用生态研究所

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