Molecular cloning and sequence analysis of the duck enteritis virus UL5 gene
文献类型：期刊论文
| 作者 | Pana, HQ ; Cao, RB ; Liu, L ; Niu, MF ; Zhou, B ; Chen, PY ; Hua, JC |
| 刊名 | VIRUS RESEARCH
 |
| 出版日期 | 2008 |
| 卷号 | 136期号:1-2页码:152-156 |
| 关键词 | duck enteritis virus UL5 gene gene character phylogenetic analysis |
| ISSN号 | 0168-1702 |
| 通讯作者 | Cao, RB, Nanjing Agr Univ, Key Lab Anim Dis Diag & Immunol, Coll Vet Med, Nanjing 210095, Peoples R China |
| 中文摘要 | Duck enteritis virus (DEV) is a herpesvirus that causes an acute, contagious, and fatal disease. In the present article, the DEV UL5 gene was cloned and sequenced from a vaccine virus. According to the consensus sequence of herpesvirus UL5 and UL3 gene degenerate oligonucleotide primers were designed and were used in the polymerase chain reaction (PCR) to amplify DNA products with 4577 bp in size. DNA sequence analysis revealed a 2568 bp open reading frame (ORF) encoding a 855 amino acid polypeptide homologous to herpesvirus UL5 proteins. The DEV UL5 gene has a base composition of 769 adenine (29.95%), 556 cytosine (21.65%),533 guanine (20.76%) and 710 thymine (27.65%). Sequence comparison revealed that the nucleotide sequence of the DEV UL5 gene was highly similar to other alphaherpesviruses. Phylogenetic tree analysis showed that the fifteen herpesviruses viruses analyzed fell into four large groups, and the duck enteritis virus itself branched and was most closely related to meleagrid herpesvirus 1, gallid herpesvirus 2 and gallid herpesvirus 3 subtrees. (c) 2008 Elsevier B.V. All rights reserved. |
| 学科主题 | Virology |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000258009100020 |
| 公开日期 | 2011-09-23 |
| 源URL | [http://210.72.129.5/handle/321005/54407]  |
| 专题 | 沈阳应用生态研究所_沈阳应用生态研究所_期刊论文 |
| 推荐引用方式 GB/T 7714 | Pana, HQ,Cao, RB,Liu, L,et al. Molecular cloning and sequence analysis of the duck enteritis virus UL5 gene[J]. VIRUS RESEARCH,2008,136(1-2):152-156. |
| APA | Pana, HQ.,Cao, RB.,Liu, L.,Niu, MF.,Zhou, B.,...&Hua, JC.(2008).Molecular cloning and sequence analysis of the duck enteritis virus UL5 gene.VIRUS RESEARCH,136(1-2),152-156. |
| MLA | Pana, HQ,et al."Molecular cloning and sequence analysis of the duck enteritis virus UL5 gene".VIRUS RESEARCH 136.1-2(2008):152-156. |
入库方式： OAI收割
来源：沈阳应用生态研究所
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