Glycerol-salt mediated stacking of nucleic acids in CZE
文献类型：期刊论文
| 作者 | Wei, HP ; Qi, C ; Xu, XM ; Zhang, ZP ; Zhou, YF ; Cui, ZQ ; Zhang, XE ; Zhang, CA |
| 刊名 | CHROMATOGRAPHIA
 |
| 出版日期 | 2008 |
| 卷号 | 67期号:5-6页码:491-494 |
| 关键词 | capillary zone electrophoresis stacking glycerol salt nucleic acids |
| ISSN号 | 0009-5893 |
| 通讯作者 | Wei, HP, Chinese Acad Sci, Wuhan Inst Virol, State Key Lab Virol, Wuhan 430071, Peoples R China |
| 中文摘要 | Nucleic acid samples with high concentrations of salt could be stacked and well separated during capillary zone electrophoresis (CZE) by adding glycerol into the samples and using a Tris-Borate-EDTA (TBE) buffer (pH 8.3) as the separation medium. The so-called glycerol-salt mediated stacking was found applicable to different types of nucleic acids. Three nucleic acids: a 16s rRNA (1,542 nt), a double stranded DNA (1.6 k bp), and a single stranded DNA (30 nt), were tested as demos in the experiments. When the sample matrix contained 50 mM KCl and 50% (w/v) glycerol, the 16s rRNA sample could be stacked as high as 30 times compared with the sample without KCl. All the nucleic acids could be stacked effectively when high concentrations of glycerol (> 50%) and salt (more than 50 mM) were present in the sample matrix, while the dsDNA could be stacked with high concentrations of glycerol (> 50%) alone. |
| 学科主题 | Biochemical Research Methods; Chemistry, Analytical |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000253624100023 |
| 公开日期 | 2011-09-23 |
| 源URL | [http://210.72.129.5/handle/321005/54507]  |
| 专题 | 沈阳应用生态研究所_沈阳应用生态研究所_期刊论文 |
| 推荐引用方式 GB/T 7714 | Wei, HP,Qi, C,Xu, XM,et al. Glycerol-salt mediated stacking of nucleic acids in CZE[J]. CHROMATOGRAPHIA,2008,67(5-6):491-494. |
| APA | Wei, HP.,Qi, C.,Xu, XM.,Zhang, ZP.,Zhou, YF.,...&Zhang, CA.(2008).Glycerol-salt mediated stacking of nucleic acids in CZE.CHROMATOGRAPHIA,67(5-6),491-494. |
| MLA | Wei, HP,et al."Glycerol-salt mediated stacking of nucleic acids in CZE".CHROMATOGRAPHIA 67.5-6(2008):491-494. |
入库方式： OAI收割
来源：沈阳应用生态研究所
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