Optimization of cellulase complex formulation for peashrub biomass hydrolysis
文献类型：期刊论文
| 作者 | Jing, DB ; Li, PJ ; Xiong, XZ ; Wang, LH |
| 刊名 | APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
 |
| 出版日期 | 2007 |
| 卷号 | 75期号:4页码:793-800 |
| 关键词 | simultaneous saccharification and fermentation cellulase-inoculant mixture peashrub woody biomass crude protein crude fiber Lactobacillus spp |
| ISSN号 | 0175-7598 |
| 通讯作者 | Jing, DB, Capital Normal Univ, Coll Life Sci, Beijing 100037, Peoples R China |
| 中文摘要 | To improve efficiency and reduce cost, solid state simultaneous saccharification and fermentation of peashrub woody biomass was investigated under anaerobic conditions at 50 degrees C, with a cellulase-inoculant mixture consisting of Trichoderma koningii cellulase, Aspergillus niger cellulase, and Lactobacillus. Experimental formulations were prepared according to uniform prescription design principles. By crude protein, crude fiber models constructed using multivariate regression in SPSS and solutions analysis through unconstrained mathematical optimization in Microsoft Excel, it was clearly revealed that low pH value (3.8) from lactic acid accumulation produced by Lactobacillus would ultimately limit enzymatic hydrolysis during long-term fermentation (30 days). It was shown that a cellulase complex with filter paper cellulase/carboxymethyl cellulase/cotton lyase/beta-glucosidase/pectinase of activity ratios of 0.6:1:0.3:1:2.6 could effectively break peashrub cell wall structure by biodegradation of easily digested components and, then, release cellular contents to improve crude protein content. Thus, the enzymatic hydrolysis of peashrub biomass by the optimized cellulase complex could improve crude protein content by 45.3% (from 8.45 to 12.28%), although it only biodegraded about 10.90% of the crude fiber (from 44.45 to 40.08%). |
| 学科主题 | Biotechnology & Applied Microbiology |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000248049100009 |
| 公开日期 | 2011-09-23 |
| 源URL | [http://210.72.129.5/handle/321005/54643]  |
| 专题 | 沈阳应用生态研究所_沈阳应用生态研究所_期刊论文 |
| 推荐引用方式 GB/T 7714 | Jing, DB,Li, PJ,Xiong, XZ,et al. Optimization of cellulase complex formulation for peashrub biomass hydrolysis[J]. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY,2007,75(4):793-800. |
| APA | Jing, DB,Li, PJ,Xiong, XZ,&Wang, LH.(2007).Optimization of cellulase complex formulation for peashrub biomass hydrolysis.APPLIED MICROBIOLOGY AND BIOTECHNOLOGY,75(4),793-800. |
| MLA | Jing, DB,et al."Optimization of cellulase complex formulation for peashrub biomass hydrolysis".APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 75.4(2007):793-800. |
入库方式： OAI收割
来源：沈阳应用生态研究所
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