Simultaneous determination of amino acids in soil by reversed phase high performance liquid chromatography by using 6-aminoquinoly-N-hydroxysuccinimidyl carbamate as a precolumn derivatization reagent
文献类型：期刊论文
| 作者 | Hou, SM ; Sun, J ; He, HB ; Zhang, XD ; Wang, YH |
| 刊名 | CHINESE JOURNAL OF ANALYTICAL CHEMISTRY
 |
| 出版日期 | 2006 |
| 卷号 | 34期号:10页码:1395-1400 |
| 关键词 | reversed phase high performance liquid chromatography pre-column derivatization 6-aminoquinoly-N-hydroxysuccinimidyl carbamate amino acids soil mass spectrometric |
| ISSN号 | 0253-3820 |
| 通讯作者 | Zhang, XD, Chinese Acad Sci, Inst Appl Ecol, Key Lab Terr Ecol Proc, Shenyang 110016, Peoples R China |
| 中文摘要 | A reversed phase high performance liquid chromatographic method for simultaneous determination of 17 amino acids in soil was established. The amino acid samples were mixed with 2-aminobutyric acid as the internal standard, derivatized with 6-aminoquinolyl-N-hydroxysuceinimidyl carbarnate (AQC), and then separated on a XTerra C(18)column using 1.8 mmol/L formate ammonium (pH = 3. 0) and acetonitrile solution as a mobile phase with gradient elution technique. The photodiode detector at 248 nm was utilized for amino acid quantification. All 17 amino acid derivatives were separated well when an optimized elution condition was applied. Consequently, good reproducibility, high sensitivity and high precision of the method were achieved. When the amino acid concentration was set at the range of 25 - 600 mu mol/L, the linear correlation coefficients of the amino acids were above 0.99 and the detector limit of each amino acids was lower than 0.5 pmol. Total ion current chromatograms of mass spectrum of 17 AQC derivatized amino acids were obtained. The method provids a new way for intensifying the study of amino acids in soil and environment. |
| 学科主题 | Chemistry, Analytical |
| 收录类别 | SCI |
| 语种 | 中文 |
| WOS记录号 | WOS:000243502600008 |
| 公开日期 | 2011-09-23 |
| 源URL | [http://210.72.129.5/handle/321005/54777]  |
| 专题 | 沈阳应用生态研究所_沈阳应用生态研究所_期刊论文 |
| 推荐引用方式 GB/T 7714 | Hou, SM,Sun, J,He, HB,et al. Simultaneous determination of amino acids in soil by reversed phase high performance liquid chromatography by using 6-aminoquinoly-N-hydroxysuccinimidyl carbamate as a precolumn derivatization reagent[J]. CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,2006,34(10):1395-1400. |
| APA | Hou, SM,Sun, J,He, HB,Zhang, XD,&Wang, YH.(2006).Simultaneous determination of amino acids in soil by reversed phase high performance liquid chromatography by using 6-aminoquinoly-N-hydroxysuccinimidyl carbamate as a precolumn derivatization reagent.CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,34(10),1395-1400. |
| MLA | Hou, SM,et al."Simultaneous determination of amino acids in soil by reversed phase high performance liquid chromatography by using 6-aminoquinoly-N-hydroxysuccinimidyl carbamate as a precolumn derivatization reagent".CHINESE JOURNAL OF ANALYTICAL CHEMISTRY 34.10(2006):1395-1400. |
入库方式： OAI收割
来源：沈阳应用生态研究所
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