Construction and functional study of a novel anti HER2/neu-hTNF-alpha immunotoxin
文献类型：期刊论文
| 作者 | Jiang, L ; Feng, J ; Wu, WF ; Yang, DL ; Yan, XY |
| 刊名 | PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS
 |
| 出版日期 | 2005 |
| 卷号 | 32期号:9页码:850-856 |
| 关键词 | anti-HER2/neu scFv C6.5 hTNF-alpha immunotoxin |
| ISSN号 | 1000-3282 |
| 通讯作者 | Wu, WF, Chinese Acad Sci, Shenyang Inst Appl Ecol, Shenyang 110016, Peoples R China |
| 中文摘要 | HER2/neu is an attractive target for tumor therapy since its overexpression in a number of tumors. In order to enhance the cytotoxicity of anti-HER2/neu antibody, and the specificity of TNF-alpha for tumor cell, a fusion gene of antiHER2-hTNF-alpha was constructed. The recombinant fusion protein was produced in E.coli, and their refolding has done through affinity chromatography on a His-column. The ELISA assay showed that antiHER2-hTNF-alpha specifically bound to HER2/neu expressing SKOV-3 and MCF-7 cells, but it did not recognize the HER2/neu negative cells A375. In vitro study, it was found that antiHER2-hTNF-alpha inhibited the proliferation of SKOV-3 and MCF-7 cells, whereas it did not effect on the A375. These studies suggest that the recombinant antiHER2-hTNF-alpha immunotoxin has a potential application in HER2/neu expressing tumor therapy. |
| 学科主题 | Biochemistry & Molecular Biology; Biophysics |
| 收录类别 | SCI |
| 语种 | 中文 |
| WOS记录号 | WOS:000232115500008 |
| 公开日期 | 2011-09-23 |
| 源URL | [http://210.72.129.5/handle/321005/55067]  |
| 专题 | 沈阳应用生态研究所_沈阳应用生态研究所_期刊论文 |
| 推荐引用方式 GB/T 7714 | Jiang, L,Feng, J,Wu, WF,et al. Construction and functional study of a novel anti HER2/neu-hTNF-alpha immunotoxin[J]. PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS,2005,32(9):850-856. |
| APA | Jiang, L,Feng, J,Wu, WF,Yang, DL,&Yan, XY.(2005).Construction and functional study of a novel anti HER2/neu-hTNF-alpha immunotoxin.PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS,32(9),850-856. |
| MLA | Jiang, L,et al."Construction and functional study of a novel anti HER2/neu-hTNF-alpha immunotoxin".PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS 32.9(2005):850-856. |
入库方式： OAI收割
来源：沈阳应用生态研究所
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