A fusion protein targeting the vascular endothelial growth factor receptor I (VEGFR-1)
文献类型：期刊论文
| 作者 | Bai, XY ; Ni, JF ; Lu, AG ; Wu, WF ; Niu, RF |
| 刊名 | PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS
 |
| 出版日期 | 2005 |
| 卷号 | 32期号:4页码:365-370 |
| 关键词 | diphtheria toxin vascular endothelial growth factor (VEGF) vascular endothelial growth factor receptor-1 fusion protein |
| ISSN号 | 1000-3282 |
| 通讯作者 | Lu, AG, Chinese Acad Sci, Inst Appl Ecol, Shenyang 110016, Peoples R China |
| 中文摘要 | Diphtheria toxin is an exotoxin secreted by Corynebacterium diphtheriae that has been lysogenized by P bacteriophage that carries the DT gene. It blocks protein synthesis and kills the target eukaryotic cell. The R82A, K84A, H86A mutant of vascular endothelial growth factor (VEGF) specially binds its receptor 1 (VEGFR-1) that is expressed highly on surface of tumor blood vessel. DT genomic DNA was extracted first and then the gene that coding the T domain and C domain of DT (DT391) were amplified. The R82A, K84A, H86A mutant were introduced to VEGF by site-directed mutagenesis. Then a VEGFR-I targeting fusion protein, DT391-mVEGF, was constructed by substituting the receptor binding domain of DT with the VEGF mutant which shows high affinity to a receptor expressed on tumor vascular. With DT391, a protein without the mVEGF domain of DT391-mVEGF, as a negative control in cytotoxicity assay, the hybrid protein DT391-mVEGF showed an inhibition to the growth of VEGFR-1 positive tumor cell. |
| 学科主题 | Biochemistry & Molecular Biology; Biophysics |
| 收录类别 | SCI |
| 语种 | 中文 |
| WOS记录号 | WOS:000228316600013 |
| 公开日期 | 2011-09-23 |
| 源URL | [http://210.72.129.5/handle/321005/55161]  |
| 专题 | 沈阳应用生态研究所_沈阳应用生态研究所_期刊论文 |
| 推荐引用方式 GB/T 7714 | Bai, XY,Ni, JF,Lu, AG,et al. A fusion protein targeting the vascular endothelial growth factor receptor I (VEGFR-1)[J]. PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS,2005,32(4):365-370. |
| APA | Bai, XY,Ni, JF,Lu, AG,Wu, WF,&Niu, RF.(2005).A fusion protein targeting the vascular endothelial growth factor receptor I (VEGFR-1).PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS,32(4),365-370. |
| MLA | Bai, XY,et al."A fusion protein targeting the vascular endothelial growth factor receptor I (VEGFR-1)".PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS 32.4(2005):365-370. |
入库方式： OAI收割
来源：沈阳应用生态研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。