Isolation and characterization of polycyclic aromatic hydrocarbons-degrading Sphingomonas sp strain ZL5
文献类型：期刊论文
| 作者 | Liu, YS ; Zhang, J ; Zhang, ZZ |
| 刊名 | BIODEGRADATION
 |
| 出版日期 | 2004 |
| 卷号 | 15期号:3页码:205-212 |
| 关键词 | biodegradation catechol 2 degradative plasmid overexpression polycyclic aromatic hydrocarbons (PAHs) Sphingomonas sp. 3-dioxygenase |
| ISSN号 | 0923-9820 |
| 通讯作者 | Zhang, J, Chinese Acad Agr Sci, Lanzhou Vet Res Inst, Dept Virus, Lanzhou 730046, Peoples R China |
| 中文摘要 | A bacterial strain ZL5, capable of growing on phenanthrene as a sole carbon and energy source but not naphthalene, was isolated by selective enrichment from crude-oil-contaminated soil of Liaohe Oil Field in China. The isolate was identified as a Sphingomonas sp. strain on the basis of 16S ribosomal DNA analysis. Strain ZL5 grown on phenanthrene exhibited catechol 2,3-dioxygenase (C23O) activity but no catechol 1,2-dioxygenase, gentisate 1,2-dioxygenase, protocatechuate 3,4-dioxygenase and protocatechuate 4,5-dioxygenase activities. This suggests that the mode of cleavage of phenanthrene by strain ZL5 could be meta via the intermediate catechol, which is different from the protocatechuate way of other two bacteria, Alcaligenes faecelis AFK2 and Nocardioides sp. strain KP7, also capable of growing on phenanthrene but not naphthalene. A resident plasmid (approximately 60 kb in size), designated as pZL, was detected from strain ZL5. Curing the plasmid with mitomycin C and transferring the plasmid to E. coli revealed that pZL was responsible for polycyclic aromatic hydrocarbons degradation. The C23O gene located on plasmid pZL was cloned and overexpressed in E. coli JM109(DE3). The ring-fission activity of the purified C23O from the recombinant E. coli on dihydroxylated aromatics was in order of catechol > 4-methylcatechol > 3-methylcatechol > 4-chlorocatechol much greater than 3,4-dihydroxyphenanthrene > 3-chlorocatechol. |
| 学科主题 | Biotechnology & Applied Microbiology |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000221202900006 |
| 公开日期 | 2011-09-23 |
| 源URL | [http://210.72.129.5/handle/321005/55305]  |
| 专题 | 沈阳应用生态研究所_沈阳应用生态研究所_期刊论文 |
| 推荐引用方式 GB/T 7714 | Liu, YS,Zhang, J,Zhang, ZZ. Isolation and characterization of polycyclic aromatic hydrocarbons-degrading Sphingomonas sp strain ZL5[J]. BIODEGRADATION,2004,15(3):205-212. |
| APA | Liu, YS,Zhang, J,&Zhang, ZZ.(2004).Isolation and characterization of polycyclic aromatic hydrocarbons-degrading Sphingomonas sp strain ZL5.BIODEGRADATION,15(3),205-212. |
| MLA | Liu, YS,et al."Isolation and characterization of polycyclic aromatic hydrocarbons-degrading Sphingomonas sp strain ZL5".BIODEGRADATION 15.3(2004):205-212. |
入库方式： OAI收割
来源：沈阳应用生态研究所
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