Expression of tryptophan decarboxylase in chloroplasts of transgenic tobacco plants
文献类型：期刊论文
| 作者 | Li, QR ; Di Fiore, S ; Fischer, R ; Wang, M |
| 刊名 | BOTANICAL BULLETIN OF ACADEMIA SINICA
 |
| 出版日期 | 2003 |
| 卷号 | 44期号:3页码:193-198 |
| 关键词 | chloroplast Nicotiana tabacum terpenoid indole alkaloids tryptophan decarboxylase |
| ISSN号 | 0006-8063 |
| 通讯作者 | Wang, M, Chinese Acad Sci, Inst Appl Ecol, Shenyang 110016, Peoples R China |
| 中文摘要 | Tryptophan decarboxylase (TDC) is a key enzyme in the biosynthesis of terpenoid indole alkaloids (TIAs) from plants such as Catharanthus roseus. We transformed tobacco plants with tdc gene and expressed chloroplast-targeted versions of TDC protein. TDC was shown to be over-expressed in chloroplasts of tobacco plants by Western blot analysis and TDC enzymatic assay. The effective expression of TDC lead to accumulation of tryptamine in transgenic tobacco plants. T-1 plants showed higher levels of TDC expression than T-0 plants. In order to confirm that TDC was correctly targeted and expressed in chloroplasts, they were isolated from T-1 transgenic tobacco plants, and the different fractions of purified chloroplasts were analyzed. Transgenic plants displayed the phenotype characteristic of necrotic syndromes, which was related to the accumulation of tryptamine. |
| 学科主题 | Plant Sciences |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000184800100003 |
| 公开日期 | 2011-09-23 |
| 源URL | [http://210.72.129.5/handle/321005/55377]  |
| 专题 | 沈阳应用生态研究所_沈阳应用生态研究所_期刊论文 |
| 推荐引用方式 GB/T 7714 | Li, QR,Di Fiore, S,Fischer, R,et al. Expression of tryptophan decarboxylase in chloroplasts of transgenic tobacco plants[J]. BOTANICAL BULLETIN OF ACADEMIA SINICA,2003,44(3):193-198. |
| APA | Li, QR,Di Fiore, S,Fischer, R,&Wang, M.(2003).Expression of tryptophan decarboxylase in chloroplasts of transgenic tobacco plants.BOTANICAL BULLETIN OF ACADEMIA SINICA,44(3),193-198. |
| MLA | Li, QR,et al."Expression of tryptophan decarboxylase in chloroplasts of transgenic tobacco plants".BOTANICAL BULLETIN OF ACADEMIA SINICA 44.3(2003):193-198. |
入库方式： OAI收割
来源：沈阳应用生态研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。