Expression of recombinant tryptophan decarboxylase in different subcellular compartments in tobacco plant
文献类型：期刊论文
| 作者 | Wang, M ; Li, QR ; Di Fiore, S ; Fischer, R |
| 刊名 | ACTA BOTANICA SINICA
 |
| 出版日期 | 2002 |
| 卷号 | 44期号:3页码:314-317 |
| 关键词 | tryptophan decarboxylase alkaloids biosynthetic pathway Nicotiana tabacum subcellular compartment |
| ISSN号 | 0577-7496 |
| 通讯作者 | Wang, M, Chinese Acad Sci, Inst Appl Ecol, Shenyang 110016, Peoples R China |
| 中文摘要 | The gene encoded for tryptophan decarboxylase (TDC), which is the key enzyme in terpenoil indole alkaloids pathway, was targeted to different subcellular compartments and stably expressed in transgenic tobacco (Nicotiana tabacum L.) plants at the levels detected by Western blot and tryptamine accumulation analysis. It was shown that the TDC was located in subcellular compartments, the chloroplasts and cytosol. The recombinant TDC targeted to chloroplasts and cytosol in tobacco plants was effectively expressed as soluble protein by Western blot analysis and enzymatic assay. The level of tryptamine accumulation in chloroplast was higher than that in cytosol and very low in vacuole and endoplasmic reticulum (ER) to be hardly detected by Western blot analysis. It was indicated that the highest amount of tryptamine was in chloroplasts, lower in endoplasmic reticula and the lowest in vacuoles as compared to those in wild type plants. The TDC targeted to different subcellular compartments of tobacco plants and its expression level were studied by different nucleotide sequences coding signal peptides at 5'-end of tdc gene in order to know the effects of the TDC in compartmentation on its functionality. |
| 学科主题 | Biochemistry & Molecular Biology; Plant Sciences |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000174617400011 |
| 公开日期 | 2011-09-23 |
| 源URL | [http://210.72.129.5/handle/321005/55507]  |
| 专题 | 沈阳应用生态研究所_沈阳应用生态研究所_期刊论文 |
| 推荐引用方式 GB/T 7714 | Wang, M,Li, QR,Di Fiore, S,et al. Expression of recombinant tryptophan decarboxylase in different subcellular compartments in tobacco plant[J]. ACTA BOTANICA SINICA,2002,44(3):314-317. |
| APA | Wang, M,Li, QR,Di Fiore, S,&Fischer, R.(2002).Expression of recombinant tryptophan decarboxylase in different subcellular compartments in tobacco plant.ACTA BOTANICA SINICA,44(3),314-317. |
| MLA | Wang, M,et al."Expression of recombinant tryptophan decarboxylase in different subcellular compartments in tobacco plant".ACTA BOTANICA SINICA 44.3(2002):314-317. |
入库方式： OAI收割
来源：沈阳应用生态研究所
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