A METHOD FOR THE QUANTIFICATION OF FRANKIA BY ESTIMATION OF HYPHAL LENGTH
文献类型：期刊论文
| 作者 | ZHANG, Z ; ZHANG, C |
| 刊名 | PROTOPLASMA
 |
| 出版日期 | 1994 |
| 卷号 | 183期号:1-4页码:182-184 |
| 关键词 | FRANKIA QUANTIFICATION HYPHAL LENGTH ACTINOMYCETE LINE INTERSECTION METHOD |
| ISSN号 | 0033-183X |
| 通讯作者 | ZHANG, Z, ACAD SINICA,INST APPL ECOL,POB 417,SHENYANG 110015,PEOPLES R CHINA |
| 中文摘要 | The cellular mass of Frankia, a filamentous actinomycete, was readily quantified by estimating hyphal length, using a modification of Tennant's method for the estimation of root length. Each sample of Frankia was stained with Coomassie Brilliant blue G 250, dispersed well, and suspended in a 0.5% agar solution. One drop of the suspension was placed in a Petroff-Hausser counting chamber with 0.05 by 0.05 mm grid squares. The number of intersections between Frankia hyphae and the grid lines in a standard area were counted under a microscope and converted to hyphal length. Using the formula: hyphal length (HL) in mm equals (11/14) times the number of intersections (n) times the grid dimension (0.05 mm). The validity of the line intersection method was tested by comparison with total protein estimates of replicate aliquots of Frankia culture. Correlations between total protein and hyphal length estimates were strong (r(2) from 0.76 to 0.95; standard errors of 3 to 9% of estimated length). These results show that line intersection counts may be a satisfactory routine method for quantifying Frankia in culture and may be especially suitable for detecting small amounts of living Frankia in less time than with other methods. |
| 学科主题 | Plant Sciences; Cell Biology |
| 收录类别 | SCI |
| 语种 | 英语 |
| 公开日期 | 2011-09-23 |
| 源URL | [http://210.72.129.5/handle/321005/55735]  |
| 专题 | 沈阳应用生态研究所_沈阳应用生态研究所_期刊论文 |
| 推荐引用方式 GB/T 7714 | ZHANG, Z,ZHANG, C. A METHOD FOR THE QUANTIFICATION OF FRANKIA BY ESTIMATION OF HYPHAL LENGTH[J]. PROTOPLASMA,1994,183(1-4):182-184. |
| APA | ZHANG, Z,&ZHANG, C.(1994).A METHOD FOR THE QUANTIFICATION OF FRANKIA BY ESTIMATION OF HYPHAL LENGTH.PROTOPLASMA,183(1-4),182-184. |
| MLA | ZHANG, Z,et al."A METHOD FOR THE QUANTIFICATION OF FRANKIA BY ESTIMATION OF HYPHAL LENGTH".PROTOPLASMA 183.1-4(1994):182-184. |
入库方式： OAI收割
来源：沈阳应用生态研究所
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