Identification of Meconopsis species by a DNA barcode sequence: The nuclear internal transcribed spacer (ITS) region of ribosomal deoxyribonucleic acid (DNA)
文献类型：期刊论文
| 作者 | Li, Rong ; Dao, Zhiling
|
| 刊名 | AFRICAN JOURNAL OF BIOTECHNOLOGY
 |
| 出版日期 | 2011-11-09 |
| 卷号 | 10期号:70页码:15805-15807 |
| 关键词 | DNA barcoding species identification Meconopsis ITS region |
| ISSN号 | 1684-5315 |
| 通讯作者 | daozhl@mail.kib.ac.cn |
| 英文摘要 | Deoxyribonucleic acid (DNA) barcoding is a novel technology that uses a standard DNA sequence to facilitate species identification. Species identification is necessary for the authentication of traditional plant based medicines. Although a consensus has not been agreed regarding which DNA sequences can be used as the best plant barcode, the internal transcribed spacer (ITS) region of nuclear ribosomal DNA has been tested and applied extensively in recent years. In this study, we used the ITS region to test its possibility to serve as barcodes for Meconopsis species which are widely used in traditional Tibetan medicines that are used throughout the Himalayas. We sequenced 14 accessions representing four Meconopsis species. The result shows ITS sequences were found to be unique for each species, with the interspecies percentages of nucleotide differences averaging 5.1% and ranging from 0.2 to 9.6%. In contrast, the intraspecies percentages of nucleotide differences among the Meconopsis species studied ranged from 0 to 1.9%. Our results indicate that the ITS region can be used as a barcode to distinguish Meconopsis species. |
| 学科主题 | Biotechnology & Applied Microbiology |
| 类目[WOS] | Biotechnology & Applied Microbiology |
| 研究领域[WOS] | Biotechnology & Applied Microbiology |
| 关键词[WOS] | LAND PLANTS ; TAXONOMY |
| 收录类别 | SCI |
| 资助信息 | Chinese Academy of Sciences[2010KIBA06] |
| 语种 | 英语 |
| WOS记录号 | WOS:000298555900021 |
| 公开日期 | 2012-03-16 |
| 源URL | [http://ir.kib.ac.cn/handle/151853/3922]  |
| 专题 | 昆明植物研究所_中国科学院东亚植物多样性与生物地理学重点实验室 |
| 作者单位 | Chinese Acad Sci, Key Lab Biodivers & Biogeog, Kunming Inst Bot, Kunming 650204, Yunnan, Peoples R China |
| 推荐引用方式 GB/T 7714 | Li, Rong,Dao, Zhiling. Identification of Meconopsis species by a DNA barcode sequence: The nuclear internal transcribed spacer (ITS) region of ribosomal deoxyribonucleic acid (DNA)[J]. AFRICAN JOURNAL OF BIOTECHNOLOGY,2011,10(70):15805-15807. |
| APA | Li, Rong,&Dao, Zhiling.(2011).Identification of Meconopsis species by a DNA barcode sequence: The nuclear internal transcribed spacer (ITS) region of ribosomal deoxyribonucleic acid (DNA).AFRICAN JOURNAL OF BIOTECHNOLOGY,10(70),15805-15807. |
| MLA | Li, Rong,et al."Identification of Meconopsis species by a DNA barcode sequence: The nuclear internal transcribed spacer (ITS) region of ribosomal deoxyribonucleic acid (DNA)".AFRICAN JOURNAL OF BIOTECHNOLOGY 10.70(2011):15805-15807. |
入库方式： OAI收割
来源：昆明植物研究所
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