A Modified Enrichment Method to Construct Microsatellite Libraryfrom Plateau Pika Genome (Ochotona curzoniae)
文献类型：期刊论文
| 作者 | Zhang Yanming
|
| 刊名 | genomics, proteomics & bioinformatics
 |
| 出版日期 | 2010 |
| 卷号 | 8期号:1页码:72-76 |
| 关键词 | microsatellite enriched library magnetic bead ultrasonication |
| 中文摘要 | a microsatellite-enriched library of plateau pika (ochotona curzoniae) was constructed according to the strongaffinity between biotin and streptavidin. firstly, genomic dna was fragmented by ultrasonication, which is a majorimprovement over traditional methods. linker-ligated dna fragments were hybridized with biotinylated microsatelliteprobes, and then were subjected to streptavidin-coated magnetic beads. pcr amplificationwasperformedto obtain double-stranded dna fragments containing microsatellites. ligation and transformation werecarried out by using the pgem-t vector system i and escherichia coli dh10b competent cells. sequencing resultsshowed that 80.2% of clones contained microsatellite repeatmotif. several modifications make this protocoltime-efficient and technically easier than the traditional ones; particularly,composition and relative abundance ofmicrosatellite repeats in plateau pika genome were truly represented through the optimized pcr conditions. thismethod has also been successfully applied to construct microsatellite-enriched genomic libraries of chinese hamster(cricetulus griseus) and small abalone [haliotis diversicolor (reeve)] with high rates of positive clones,demonstrating its feasibility and stability. |
| 收录类别 | CSCD |
| 公开日期 | 2011-12-09 |
| 源URL | [http://ir.nwipb.ac.cn//handle/363003/1492]  |
| 专题 | 西北高原生物研究所_中国科学院西北高原生物研究所 |
| 推荐引用方式 GB/T 7714 | Zhang Yanming. A Modified Enrichment Method to Construct Microsatellite Libraryfrom Plateau Pika Genome (Ochotona curzoniae)[J]. genomics, proteomics & bioinformatics,2010,8(1):72-76. |
| APA | Zhang Yanming.(2010).A Modified Enrichment Method to Construct Microsatellite Libraryfrom Plateau Pika Genome (Ochotona curzoniae).genomics, proteomics & bioinformatics,8(1),72-76. |
| MLA | Zhang Yanming."A Modified Enrichment Method to Construct Microsatellite Libraryfrom Plateau Pika Genome (Ochotona curzoniae)".genomics, proteomics & bioinformatics 8.1(2010):72-76. |
入库方式： OAI收割
来源：西北高原生物研究所
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