Generation of reactive oxygen species in cyanobacteria and green algae induced by allelochemicals of submerged macrophytes
文献类型：期刊论文
| 作者 | Wang, Jing1,2; Zhu, Junying1,2; Liu, Shaoping3; Liu, Biyun1; Gao, Yunni1; Wu, Zhenbin1 |
| 刊名 | CHEMOSPHERE
 |
| 出版日期 | 2011-10-01 |
| 卷号 | 85期号:6页码:977-982 |
| 关键词 | Pyrogallic acid (+)-Catechin Reactive oxygen species Phytoplankton Redox cycling |
| ISSN号 | 0045-6535 |
| 通讯作者 | Wu, Z.(wuzb@ihb.ac.cn) |
| 中文摘要 | Inhibition of phytoplankton by allelochemicals released by submerged macrophytes is reported to be one of the mechanisms that maintain a clear-water state in shallow lakes. In order to elucidate this mechanism, the ability of six polyphenols and two long-chain fatty acids to induce the generation of reactive oxygen species (ROS) in phytoplankton was studied using the ROS sensitive probe 2',7'- dichlorodihydrofluorescein diacetate (DCFH-DA). The results showed that only (+)-catechin (CA) and pyrogallic acid (PA) could induce ROS formation in Microcystis aeruginosa and Pseudokirchneriella subcapitata. 25mgL-1 CA caused 1.2, 1.4 and 1.8 times increase of ROS levels in M. aeruginosa at 1, 2 and 4h exposure, respectively, and, correspondingly in P. subcapitata cells, these values were 3.7, 6.2 and 7.7, respectively. PA also significantly increased the levels of intracellular ROS in P. subcapitata (P<0.01); however, significant ROS generation in M. aeruginosa was observed at only 4h exposure (P<0.01). Light enhanced ROS generation in CA treated cells, but not in the cells treated with PA. CA and PA may act as redox cyclers after uptake by test organisms and produce ROS successively. These results suggest that the oxidative stress induced by the redox cycling property of allelochemicals may be one of the important causes for the inhibitory effect of some submerged macrophytes towards undesired phytoplankton in natural aquatic ecosystems. |
| 英文摘要 | Inhibition of phytoplankton by allelochemicals released by submerged macrophytes is reported to be one of the mechanisms that maintain a clear-water state in shallow lakes. In order to elucidate this mechanism, the ability of six polyphenols and two long-chain fatty acids to induce the generation of reactive oxygen species (ROS) in phytoplankton was studied using the ROS sensitive probe 2',7'- dichlorodihydrofluorescein diacetate (DCFH-DA). The results showed that only (+)-catechin (CA) and pyrogallic acid (PA) could induce ROS formation in Microcystis aeruginosa and Pseudokirchneriella subcapitata. 25 mg L(-1) CA caused 1.2, 1.4 and 1.8 times increase of ROS levels in M. aeruginosa at 1,2 and 4 h exposure, respectively, and, correspondingly in P. subcapitata cells, these values were 3.7, 6.2 and 7.7, respectively. PA also significantly increased the levels of intracellular ROS in P. subcapitata (P < 0.01); however, significant ROS generation in M. aeruginosa was observed at only 4 h exposure (P < 0.01). Light enhanced ROS generation in CA treated cells, but not in the cells treated with PA. CA and PA may act as redox cyclers after uptake by test organisms and produce ROS successively. These results suggest that the oxidative stress induced by the redox cycling property of allelochemicals may be one of the important causes for the inhibitory effect of some submerged macrophytes towards undesired phytoplankton in natural aquatic ecosystems. (C) 2011 Elsevier Ltd. All rights reserved. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Environmental Sciences |
| 研究领域[WOS] | Environmental Sciences & Ecology |
| 关键词[WOS] | MYRIOPHYLLUM-SPICATUM ; (-)-EPIGALLOCATECHIN GALLATE ; GROWTH-INHIBITION ; HYDROGEN-PEROXIDE ; MICROCYSTIS-AERUGINOSA ; PHYTOPLANKTON ; MECHANISMS ; PYROGALLOL ; POLYPHENOLS ; INVOLVEMENT |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000298223400009 |
| 公开日期 | 2012-03-20 |
| 源URL | [http://ir.ihb.ac.cn/handle/342005/16682]  |
| 专题 | 水生生物研究所_水环境工程研究中心_期刊论文 |
| 作者单位 | 1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China 3.Wuhan Univ, Med Res Ctr Zhongnan Hosp, Wuhan 430071, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Jing,Zhu, Junying,Liu, Shaoping,et al. Generation of reactive oxygen species in cyanobacteria and green algae induced by allelochemicals of submerged macrophytes[J]. CHEMOSPHERE,2011,85(6):977-982. |
| APA | Wang, Jing,Zhu, Junying,Liu, Shaoping,Liu, Biyun,Gao, Yunni,&Wu, Zhenbin.(2011).Generation of reactive oxygen species in cyanobacteria and green algae induced by allelochemicals of submerged macrophytes.CHEMOSPHERE,85(6),977-982. |
| MLA | Wang, Jing,et al."Generation of reactive oxygen species in cyanobacteria and green algae induced by allelochemicals of submerged macrophytes".CHEMOSPHERE 85.6(2011):977-982. |
入库方式： OAI收割
来源：水生生物研究所
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