Preparation of protoplasts from Chlorella protothecoides
文献类型：期刊论文
| 作者 | Lu, Yongzhong1; Kong, Renqiu2; Hu, Liwei1 |
| 刊名 | WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY
 |
| 出版日期 | 2012-04-01 |
| 卷号 | 28期号:4页码:1827-1830 |
| 关键词 | Protoplast Cell wall Enzymatic digestion Chlorella Regeneration |
| ISSN号 | 0959-3993 |
| 通讯作者 | Lu, Y.(luyz@yahoo.com) |
| 中文摘要 | This paper describes an enzymatic method for yielding protoplasts from the microalga Chlorella protothecoides. Four kinds of commercially available enzymes were tested. The enzymatic digestion was optimal with 2% cellulase R-10 and 1% snailase prepared in 25 mM Tris buffer (pH 6.0) containing 0.6 M D-mannitol, and the protoplast density could reach the peak after treatment at 30°C for 16 h. Nearly all liberated protoplasts were green in the presence of 0.01% phenosafranin, indicating their high viability. The regeneration rate was about 70% when 0.6 M D-mannitol was used as an osmotic stabilizer in the regeneration medium. This protocol will find useful applications in genetic studies of this algal species. |
| 英文摘要 | This paper describes an enzymatic method for yielding protoplasts from the microalga Chlorella protothecoides. Four kinds of commercially available enzymes were tested. The enzymatic digestion was optimal with 2% cellulase R-10 and 1% snailase prepared in 25 mM Tris buffer (pH 6.0) containing 0.6 M D-mannitol, and the protoplast density could reach the peak after treatment at 30A degrees C for 16 h. Nearly all liberated protoplasts were green in the presence of 0.01% phenosafranin, indicating their high viability. The regeneration rate was about 70% when 0.6 M D-mannitol was used as an osmotic stabilizer in the regeneration medium. This protocol will find useful applications in genetic studies of this algal species. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Biotechnology & Applied Microbiology |
| 研究领域[WOS] | Biotechnology & Applied Microbiology |
| 关键词[WOS] | CELL-WALL ; INDUCTION ; ALGAE |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000302292500054 |
| 公开日期 | 2012-03-20 |
| 源URL | [http://ir.ihb.ac.cn/handle/342005/16686]  |
| 专题 | 水生生物研究所_藻类生物学及应用研究中心_期刊论文 |
| 作者单位 | 1.Qingdao Univ Sci & Technol, Dept Biol, Qingdao 266042, Peoples R China 2.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430071, Peoples R China |
| 推荐引用方式 GB/T 7714 | Lu, Yongzhong,Kong, Renqiu,Hu, Liwei. Preparation of protoplasts from Chlorella protothecoides[J]. WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY,2012,28(4):1827-1830. |
| APA | Lu, Yongzhong,Kong, Renqiu,&Hu, Liwei.(2012).Preparation of protoplasts from Chlorella protothecoides.WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY,28(4),1827-1830. |
| MLA | Lu, Yongzhong,et al."Preparation of protoplasts from Chlorella protothecoides".WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY 28.4(2012):1827-1830. |
入库方式： OAI收割
来源：水生生物研究所
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