Serum tumor marker detection on PEGylated lipid membrane using biosensor based on total internal reflection imaging ellipsometry
文献类型：期刊论文
| 作者 | Zhang YB; Chen YY(陈艳艳) ; Jin G(靳刚)
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| 刊名 | Sensors and Actuators B-Chemical
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| 出版日期 | 2011 |
| 卷号 | 159期号:1页码:121-125 |
| 通讯作者邮箱 | yychen2006@sinano.ac.cn; gajin@imech.ac.cn |
| 关键词 | Pegylated Phospholipid Membrane Total Internal Reflection Imaging Ellipsometry Ca19-9 Resectable Pancreatic Adenocarcinoma Perioperative Ca19-9 Levels (18)Fdg-Pet Examination Predict Stage Protein Adsorption Immobilization Surfaces Antigen Cancer |
| ISSN号 | 0925-4005 |
| 产权排序 | [Zhang, Yibang; Chen, Yanyan; Jin, Gang] Chinese Acad Sci, Suzhou Inst Nanotech & Nanobion, Suzhou 215125, Jiangsu, Peoples R China; [Zhang, Yibang; Jin, Gang] Chinese Acad Sci, Inst Biophys, Beijing 100101, Peoples R China; [Zhang, Yibang] Chinese Acad Sci, Grad Univ, Suzhou 100049, Peoples R China; [Jin, Gang] Chinese Acad Sci, Inst Mech, Beijing 100190, Peoples R China |
| 通讯作者 | Chen, YY (reprint author), Chinese Acad Sci, Suzhou Inst Nanotech & Nanobion, Suzhou 215125, Jiangsu, Peoples R China |
| 合作状况 | 国内 |
| 中文摘要 | We describe highly sensitive, label-free, real time detection of the biomarker CA19-9 using microarray biosensors based on total internal reflection imaging ellipsometry (TIRIE), in which anti-CA19-9 is immobilized onto the PEGylated phospholipid membrane. Strong resistance against non-specific adsorption of PEGylated lipid membrane allows direct serum assay without the blocking agent. We find that PEG density has an influence on antibody assembly and only a proper amount of PEG is appropriate for antigen binding. Two different methods for antibody assembly are compared and finally protein A is preferred due to its higher protein binding efficiency. The estimated detection limit of CA19-9 is 18.2 U/ml, which is lower than the cut-off value for normal level (37 U/ml). Regression analysis between the results of microarray biosensor and electrochemiluminescence immunoassay (ECLIA) suggests a high correlation (R = 0.989). The capability for real-time monitoring of tumor marker with high sensitivity and selectivity in clinically relevant samples opens up substantial possibilities for early cancer diagnosis and treatment. (C) 2011 Elsevier B.V. All rights reserved. |
| 学科主题 | Chemistry; Electrochemistry; Instruments & Instrumentation |
| 分类号 | 二类/Q1 |
| 类目[WOS] | Chemistry, Analytical ; Electrochemistry ; Instruments & Instrumentation |
| 研究领域[WOS] | Chemistry ; Electrochemistry ; Instruments & Instrumentation |
| 关键词[WOS] | RESECTABLE PANCREATIC ADENOCARCINOMA ; PERIOPERATIVE CA19-9 LEVELS ; (18)FDG-PET EXAMINATION ; PREDICT STAGE ; PROTEIN ; ADSORPTION ; IMMOBILIZATION ; SURFACES ; ANTIGEN ; CANCER |
| 收录类别 | SCI ; EI |
| 资助信息 | The authors would like to gratefully acknowledge financial supports from Ministry of Science and Technology of China (2008AA02Z419, 2009CB320302), Natural Science Foundation (10804083, 20845003) of China, Chinese Academy of Sciences (KJCX2-YW-M04, KJCX2-YW-M03) and Key Laboratory of Microgravity, Institute of Mechanics, CAS. |
| 原文出处 | http://dx.doi.org/10.1016/j.snb.2011.06.059 |
| 语种 | 英语 |
| WOS记录号 | WOS:000295745900017 |
| 公开日期 | 2012-04-01 |
| 源URL | [http://dspace.imech.ac.cn/handle/311007/45066]  |
| 专题 | 力学研究所_国家微重力实验室 |
| 推荐引用方式 GB/T 7714 | Zhang YB,Chen YY,Jin G. Serum tumor marker detection on PEGylated lipid membrane using biosensor based on total internal reflection imaging ellipsometry[J]. Sensors and Actuators B-Chemical,2011,159(1):121-125. |
| APA | Zhang YB,陈艳艳,&靳刚.(2011).Serum tumor marker detection on PEGylated lipid membrane using biosensor based on total internal reflection imaging ellipsometry.Sensors and Actuators B-Chemical,159(1),121-125. |
| MLA | Zhang YB,et al."Serum tumor marker detection on PEGylated lipid membrane using biosensor based on total internal reflection imaging ellipsometry".Sensors and Actuators B-Chemical 159.1(2011):121-125. |
入库方式： OAI收割
来源：力学研究所
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