Modeling of Cell Aggregation Dynamics Governed by Receptor-Ligand Binding Under Shear Flow
文献类型：期刊论文
| 作者 | Fu ZL(傅长亮) ; Tong CF(佟春芳); Dong C; Long M(龙勉)
|
| 刊名 | Cellular and Molecular Bioengineering
 |
| 出版日期 | 2011 |
| 卷号 | 4期号:3页码:427-441 |
| 通讯作者邮箱 | mlong@imech.ac.cn |
| 关键词 | Two-Dimensional Kinetics Cone-Plate Viscometer Homotypic Aggregation Heterotypic Aggregation Bell Model Protein G-Igg Bond Beta(2)-Integrin And Icam-1 Bond Human Blood-Platelets Intercellular-Adhesion Molecule-1 Hydrodynamic Shear Disaggregation Kinetics Neutrophil Aggregation Sequential Binding Mediated Adhesion Stable Adhesion Melanoma-Cells Latex Spheres |
| ISSN号 | 1865-5025 |
| 产权排序 | [Fu, Changliang; Tong, Chunfang; Long, Mian] Chinese Acad Sci, Key Lab Micrograv, Inst Mech, Beijing 100190, Peoples R China; [Fu, Changliang; Tong, Chunfang; Long, Mian] Chinese Acad Sci, Natl Micrograv Lab, Inst Mech, Beijing 100190, Peoples R China; [Fu, Changliang; Tong, Chunfang; Long, Mian] Chinese Acad Sci, Ctr Biomech & Bioengn, Inst Mech, Beijing 100190, Peoples R China; [Dong, Cheng] Penn State Univ, Dept Bioengn, University Pk, PA 16802 USA |
| 通讯作者 | Long, M (reprint author), Chinese Acad Sci, Key Lab Micrograv, Inst Mech, Beijing 100190, Peoples R China |
| 合作状况 | 国际 |
| 中文摘要 | Shear-induced cell aggregation and disaggregation, governed by specific receptor-ligand binding, play important roles in many biological and biophysical processes. While a lot of studies have focused on elucidating the shear rate and shear stress dependence of cell aggregation, the majority of existing models based on population balance equation (PBE) has rarely dealt with cell aggregation dynamics upon intrinsic molecular kinetics. Here, a kinetic model was developed for further understanding cell aggregation and disaggregation in a linear shear flow. The novelty of the model is that a set of simple equations was constructed by coupling two-body collision theory with receptor-ligand binding kinetics. Two cases of study were employed to validate the model: one is for the homotypic aggregation dynamics of latex beads cross-linked by protein G-IgG binding, and the other is for the heterotypic aggregation dynamics of neutrophils-tumor cells governed by beta 2-integrin-ligand interactions. It was found that the model fits the data well and the obtained kinetic parameters are consistent with the previous predictions and experimental measurements. Moreover, the decay factor defined biophysically to account for the chemokine- and shear-induced regulation of receptor and/or ligand expression and conformation was compared at molecular and cellular levels. Our results provided a universal framework to quantify the molecular kinetics of receptor-ligand binding in shear-induced cell aggregation dynamics. |
| 学科主题 | Cell Biology; Biophysics |
| 分类号 | Q4 |
| 类目[WOS] | Cell & Tissue Engineering ; Biophysics ; Cell Biology |
| 研究领域[WOS] | Cell Biology ; Biophysics |
| 关键词[WOS] | HUMAN BLOOD-PLATELETS ; INTERCELLULAR-ADHESION MOLECULE-1 ; HYDRODYNAMIC SHEAR ; DISAGGREGATION KINETICS ; NEUTROPHIL AGGREGATION ; SEQUENTIAL BINDING ; MEDIATED ADHESION ; STABLE ADHESION ; MELANOMA-CELLS ; LATEX SPHERES |
| 收录类别 | SCI |
| 资助信息 | This work was supported by National Natural Science Foundation of China grants 30730032, 11072251, 10902117, and 10702075, Chinese Academy of Sciences grants KJCX2-YW-L08 and Y2010030, and National Key Basic Research Foundation of China grant 2011CB710904. |
| 原文出处 | http://dx.doi.org/10.1007/s12195-011-0167-x |
| 语种 | 英语 |
| WOS记录号 | WOS:000297866300011 |
| 公开日期 | 2012-04-01 |
| 源URL | [http://dspace.imech.ac.cn/handle/311007/45075]  |
| 专题 | 力学研究所_国家微重力实验室 |
| 推荐引用方式 GB/T 7714 | Fu ZL,Tong CF,Dong C,et al. Modeling of Cell Aggregation Dynamics Governed by Receptor-Ligand Binding Under Shear Flow[J]. Cellular and Molecular Bioengineering,2011,4(3):427-441. |
| APA | 傅长亮,佟春芳,Dong C,&龙勉.(2011).Modeling of Cell Aggregation Dynamics Governed by Receptor-Ligand Binding Under Shear Flow.Cellular and Molecular Bioengineering,4(3),427-441. |
| MLA | 傅长亮,et al."Modeling of Cell Aggregation Dynamics Governed by Receptor-Ligand Binding Under Shear Flow".Cellular and Molecular Bioengineering 4.3(2011):427-441. |
入库方式： OAI收割
来源：力学研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。